JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (3): 37-39.doi: 10.6040/j.issn.1671-7554.0.2013.495

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Effect of hepatitis C virus core protein on the apoptosis of human hepatocytes

CAO Tong1, WANG Yiguo2   

  1. 1. Shandong Academy of Medical Science, School of Medicine and Life Sciences,
    Jinan University, Jinan 250062, Shandong, China; 2. Department of Gastroenterology,
    Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2013-08-12 Online:2014-03-10 Published:2014-03-10

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Abstract:

Objective  To investigate the effects of hepatitis C virus (HCV) core protein on the apoptosis of human hepatocytes. Methods  The eukaryotic expressing vector for HCV core protein was constructed, and transfected with QSG7701 cells. The expressions of core protein and NF-κB were detected by Western blotting. The apoptosis rate of QSG7701 cells was measured with flow cytometry. Results  The apoptosis of QSG7701/core was (1.34±0.07)%, lower than (2.35±0.11)% of QSG7701 and (2.58±0.13)% of QSG7701/pcDNA-3.1, with statistical significant difference (P<0.05). Besides, the transcription factor NF-κB was activated.  Conclusion  HCV core protein suppresses apoptosis of QSG7701 possibly by activating the transcription factor NF-κB.

Key words: Hepatocytes,  Hepatitis C virus, NF-κB, Apoptosis, Transfection

CLC Number: 

  • R512.6
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