MiR-125a-3p对类风湿关节炎滑膜成纤维细胞增殖的影响

doi:10.6040/j.issn.1671-7554.0.2025.0692

摘要/Abstract

摘要： 目的探讨miR-125a-3p对类风湿关节炎滑膜成纤维细胞(rheumatoid arthritis synovial fibroblasts, RA-FLS)增殖能力及炎症因子分泌水平的影响。方法选取类风湿关节炎(rheumatoid arthritis, RA)患者25例,无菌获得其关节置换术后的滑膜组织,分离出RA-FLS用于实验研究。通过细胞转染将miR-125a-3p mimics及miR-NC转染到RA-FLS中进行细胞培养,将培养细胞分为Control组、miR-NC组及miR-125a-3p mimics组,实时定量PCR(real-time quantitative PCR,RT-qPCR)法检测各组细胞中miR-125a-3p的表达,细胞计数试剂盒-8(cell-counting-kit-8, CCK-8)测定细胞增殖能力,酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)检测细胞培养上清液中肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、白细胞介素-6(interleukin-6, IL-6)和白细胞介素-1β(interleukin-1β, IL-1β)的水平。结果 miR-125a-3p mimics组RA-FLS的细胞活力以及细胞培养上清液中TNF-a、IL-1β和IL-6的水平较miR-NC组下降,差异有统计学意义(P<0.001)。结论 miR-125a-3p抑制RA-FLS增殖及炎症因子的分泌,可能成为研究RA发病机制的新靶点。

关键词: 类风湿关节炎, 滑膜成纤维细胞, 微小RNA, miR-125a-3p

Abstract: Objective To investigate the effects of miR-125a-3p on the proliferation capacity and inflammatory factor secretion levels in rheumatoid arthritis synovial fibroblasts(RA-FLS). Methods Synovial tissues were aseptically obtained from 25 patients with rheumatoid arthritis(RA)after joint replacement surgery, and RA-FLS were isolated for experimental study. MiR-125a-3p mimics and miR-NC were transfected into RA-FLS via cell transfection, and the cultured cells were divided into Control, miR-NC, and miR-125a-3p mimics groups. The expression of miR-125a-3p in each group was detected by real-time quantitative PCR(RT-qPCR). Cell counting kit-8(CCK-8)was used to determine the cell proliferation capacity, and enzyme-linked immunosorbent assay(ELISA)was employed to detect the levels of tumor necrosis factor α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β)in the cell culture supernatant. Results The cell viability of RA-FLS and the levels of TNF-α, IL-1β, and IL-6 in the cell culture supernatant in the miR-125a-3p mimics group were significantly lower than those in the miR-NC group(P<0.001). Conclusion MiR-125a-3p inhibits the proliferation of RA-FLS and the secretion of inflammatory factors, potentially serving as a new target for studying the pathogenesis of RA.

Key words: Rheumatoid arthritis, Synovial fibroblasts, MicroRNA, MiR-125a-3p

中图分类号:

R684.3

宋慧梳,孙志坚,张邱婷,弭雪,张妮,王志仑,尹哲,郝学喜,王迎雪. MiR-125a-3p对类风湿关节炎滑膜成纤维细胞增殖的影响[J]. 山东大学学报 (医学版), 2025, 63(12): 74-78.

SONG Huishu, SUN Zhijian, ZHANG Qiuting, MI Xue, ZHANG Ni, WANG Zhilun, YIN Zhe, HAO Xuexi, WANG Yingxue. Effet of miR-125a-3p on the proliferation of rheumatoid arthritis synovial fibroblasts[J]. Journal of Shandong University (Health Sciences), 2025, 63(12): 74-78.

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