筛选类风湿关节炎中糖代谢关键基因的探讨

doi:10.6040/j.issn.1671-7554.0.2015.842

摘要/Abstract

摘要： 目的探讨糖代谢关键酶在类风湿关节炎(RA)中的表达,进一步探讨糖代谢与RA的相关性。方法取牛Ⅱ型胶原诱导大鼠(CIA组)和正常大鼠(NCR组)膝关节滑膜组织,提取总RNA,采用Rat Glucose Metabolism RT² Profiler^TM PCR Array筛选表达不一致基因;采用RT-PCR法对CIA组和NCR组进行mRNA水平的检测,采用RT-PCR法和Western blotting法检测RA组和骨关节炎组(OA组)滑膜组织中基因mRNA水平和蛋白水平的表达。收集RA血清(RAB组)和正常献血者血清(NCB组),采用ELISA法检测血清中的表达水平。结果 PCR Array检测显示,CIA组烯醇化酶(ENO1)、己糖激酶2(HK2)、磷酸甘油酸激酶-1(PGK1)较NCR组表达上调(P<0.05),磷酸烯醇式丙酮酸羧激酶-1(PCK1)和丙酮酸脱氢酶激酶-4(PDK4)表达下调(P<0.05); RT-PCR法和Western blotting法检测显示,RA组与OA组相比, ENO1和PGK1表达上调(P<0.05),ELISA法检测结果显示,RAB组与NCB组相比,PGK1表达升高(P<0.05)。结论牛Ⅱ型胶原诱导大鼠和RA滑膜组织中糖代谢关键酶ENO1和PGK1表达增高,RA血液中PGK1表达增加,均提示RA中糖代谢活动增强,导致相关基因的表达异常。

关键词: 类风湿关节炎, 磷酸甘油酸酯激1, 葡萄糖代谢, 胶原诱导型关节炎, 烯醇化酶α

Abstract: Objective To determine the crucial enzymes of glucose metabolism in rheumatoid arthritis(RA). Methods The glucose metabolism in synovial tissues of collagen-induced arthritis(CIA)was analyzed with Rat Glucose Metabolism RT² Profiler^TM PCR Array. The result was verified using real time PCR, Western blotting and ELISA in synovial tissues and blood from patients with RA. Results The PCR array detected up-regulation of ENO1, HK2 and PGK1, and down-regulation of PCK1 and PDK4 in synovial tissues of CIA rats. Real time PCR and Western blotting detected increased expressions of ENO1 and PGK1 in synovial tissues of RA. ELISA detected a high level of PGK1 in blood of RA patients. The result identified the increased expression of ENO1 and PGK1 in CIA and RA synovial tissues, and elevated level of PGK1 in blood of the patients with RA. Conclusion Glycolytic activity is increased in RA, which may result in the abnormal expression of genes involved.

Key words: Rheumatoid arthritis, Collagen-inducing arthritis, Enolase1(alpha), Glucosemetabolism, Phosphoglycerate kinase 1

中图分类号:

R593.22

赵琰,郑亚冰,闫新峰,张虎,常晓天. 筛选类风湿关节炎中糖代谢关键基因的探讨[J]. 山东大学学报（医学版）, 2016, 54(3): 30-35.

ZHAO Yan, ZHENG Yabing, YAN Xinfeng, ZHANG Hu, CHANG Xiaotian. Screening crucial genes for glucose metabolism in rheumatoid arthritis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 30-35.

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