山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (5): 68-72, 81.doi: 10.6040/j.issn.1671-7554.0.2021.0265
赵怀龙,吕燕,赵红,赵宝添,韩莹,潘晶,丁小满,王春荣,韩秀云,刘岚铮*(
)
Huailong ZHAO,Yan LYU,Hong ZHAO,Baotian ZHAO,Ying HAN,Jing PAN,Xiaoman DING,Chunrong WANG,Xiuyun HAN,Lanzheng LIU*()
摘要：
目的: 对济南市2020年常规监测鉴定的人鼻病毒(HRV)进行基因组测序并分析其特征及变异情况。方法: 荧光定量RT-PCR方法对流感监测点采集的流感样病例样本进行筛查，进而通过宏基因组测序方法获得基因组序列，利用Lasergene和MEGA等软件进行分子变异及系统进化分析。结果: HRV JN909株基因组序列全长7219 nt，为鼻病毒B83型。根据VP4/VP2基因序列建立的系统发育树及主要结构蛋白基因与氨基酸同源性比对，JN909株与2019年美国西雅图来源的HRV SC0340株(GenBank登录号：MN306025)亲缘关系较近，其开读框(ORF)核苷酸与氨基酸序列的同源性分别为98.5%与99.7%，二者仅有6个氨基酸残基位点发生变异，其中VP1蛋白发生A23T和P275L变异。结论: HRV JN909株与美国SC0340株具有极高的同源性，共发生6个氨基酸位点变异。
中图分类号:
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