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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (5): 68-72, 81.doi: 10.6040/j.issn.1671-7554.0.2021.0265

• 医学病毒的基础与临床研究进展专题 • 上一篇    下一篇

济南市人鼻病毒B83型基因组特征及分子变异分析

赵怀龙,吕燕,赵红,赵宝添,韩莹,潘晶,丁小满,王春荣,韩秀云,刘岚铮*()   

  1. 济南市疾病预防控制中心, 山东 济南 250021
  • 收稿日期:2021-03-12 出版日期:2021-05-10 发布日期:2021-06-01
  • 通讯作者: 刘岚铮 E-mail:jncdcllz@jn.shandong.cn
  • 基金资助:
    济南市卫生健康委科技计划(2020-4-118)

Genetic characteristics and molecular variation of human rhinovirus genetype B83 in Jinan

Huailong ZHAO,Yan LYU,Hong ZHAO,Baotian ZHAO,Ying HAN,Jing PAN,Xiaoman DING,Chunrong WANG,Xiuyun HAN,Lanzheng LIU*()   

  1. Jinan Center for Disease Control and Prevention, Jinan 250021, Shandong, China
  • Received:2021-03-12 Online:2021-05-10 Published:2021-06-01
  • Contact: Lanzheng LIU E-mail:jncdcllz@jn.shandong.cn

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摘要：

目的: 对济南市2020年常规监测鉴定的人鼻病毒(HRV)进行基因组测序并分析其特征及变异情况。方法: 荧光定量RT-PCR方法对流感监测点采集的流感样病例样本进行筛查，进而通过宏基因组测序方法获得基因组序列，利用Lasergene和MEGA等软件进行分子变异及系统进化分析。结果: HRV JN909株基因组序列全长7219 nt，为鼻病毒B83型。根据VP4/VP2基因序列建立的系统发育树及主要结构蛋白基因与氨基酸同源性比对，JN909株与2019年美国西雅图来源的HRV SC0340株(GenBank登录号：MN306025)亲缘关系较近，其开读框(ORF)核苷酸与氨基酸序列的同源性分别为98.5%与99.7%，二者仅有6个氨基酸残基位点发生变异，其中VP1蛋白发生A23T和P275L变异。结论: HRV JN909株与美国SC0340株具有极高的同源性，共发生6个氨基酸位点变异。

关键词: 人鼻病毒, 系统进化树, 基因组, 突变

Abstract:

Objective: To analyze the genetic characteristics and molecular variation of human rhinovirus (HRV) identified in Jinan, 2020. Methods: The samples collected form influenza-like illness were tested by realtime RT-PCR and the HRV nucleic acid positive sample was sequenced with metagenome sequencing technology. Molecular variation and phylogenetic trees of VP4/VP2 coding region were analyzed using the softwares Lasergene 7.1 and MEGA 5.2. Results: The 7 219 nt complete genome sequence of JN909 was obtained. Phylogenetic analysis of VP4/VP2 coding region on HRV positive strain agreed with the serotype HRV B83. The JN909 was genetically closed to the SC0340 strain found in Seattle City of USA (GenBank accession number: MN306025), with a nucleotide similarity of 98.5% and deduced amino acid similarity of 99.7% of ORF, and there were 6 sites mutation between stains, in which substitutions A23T and P275L sites were located in VP1. Conclusion: There is a high homology between JN909 and SC0340 strains, and only 6 sites amino acid mutations occurs.

Key words: Human rhinovirus, Phylogenetic analysis, Genome, Mutation

中图分类号: 

  • R183.3

图1

2020年济南市人鼻病毒VP4/VP2基因系统进化树 ●济南样本株"

表1

JN909与人鼻病毒B83型核苷酸及氨基酸序列一致性分析"

GenBank登录号 时间 国别/地区 血清型序列一致性(nt/aa)%
ORF 5’UTR VP4 VP2 VP3 VP1
MN306025 2019 美国 B83 98.5/99.7 99.7 99.0/100.0 99.0/100.0 98.3/100.0 97.5/99.3
MF160982 2014 美国 B83 — — 99.0/100.0 98.2/100.0 — —
FJ445161 2008 美国 B83 91.4/98.2 97.1 94.7/98.6 92.0/98.1 90.8/99.6 90.5/95.5
JN990701 2007 美国 B83 96.4/99.3 99.4 98.1/100.0 97.5/99.6 95.9/100.0 96.7/99.7
JX863847 2010 中国/上海 B83 — — 96.1/98.6 93.9/98.1 — —
MH648108 2010 新加坡 B83 — 87.1 84.1/97.1 83.4/92.5 — —
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