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山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (3): 72-78.doi: 10.6040/j.issn.1671-7554.0.2017.1191

• • 上一篇    

脐带和脂肪源性间充质干细胞生物学特性比较

黄平1,张坤1,2,李芳1,2,黄国宝3,延冰4,肖东杰1,2,汪运山1,2,刘华1,2   

  1. 1. 山东大学附属济南市中心医院医学实验诊断中心, 山东 济南 250013;2. 山东省移植与组织工程技术研究中心, 山东 济南 250013; 3. 山东大学附属济南市中心医院烧伤整复外科, 山东 济南 250013;4. 山东大学附属济南市中心医院胃肠外科, 山东 济南 250013
  • 收稿日期:2017-12-01 发布日期:2022-09-27
  • 通讯作者: 刘华. E-mail:liuhuagreen@126.com
  • 基金资助:
    国家自然科学基金青年科学基金(81200950);山东省科技发展计划(2014GSF118173);中国博士后科学基金面上项目(2016M590637);山东省医药卫生科技发展计划(2016WS0139,2016WS0134)

Comparative study on biological characteristics of mesenchymal stem cells from human umbilical cord and adipose

HUANG Ping1, ZHANG Kun1,2, LI Fang1,2, HUANG Guobao3, YAN Bing4,XIAO Dongjie1,2, WANG Yunshan1,2, LIU Hua1,2   

  1. 1. Medical Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Shandong Research Center of Transplantation and Tissue, Jinan 250013, Shandong, China;
    3. Department of Burn and Plastic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    4. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2017-12-01 Published:2022-09-27

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摘要： 目的 比较脐带间充质干细胞(UC-MSCs)与脂肪源性间充质干细胞(AD-MSCs)的体外生物学特性。 方法 分离培养UC-MSCs与AD-MSCs,CCK-8法检测UC-MSCs与AD-MSCs的增殖能力,流式细胞术检测细胞表面标志物表达。油红O,茜红素及Von Kossa 染色比较UC-MSCs与AD-MSCs成脂成骨诱导分化能力。MSCs与外周血单个核细胞(PBMC)共培养,在植物血凝素(PHA)刺激作用下,流式细胞术分析MSCs对PBMC增殖抑制作用。 结果 生长曲线结果显示,UC-MSCs细胞增殖优于AD-MSCs,UC-MSCs与AD-MSCs稳定表达CD13、CD44、CD73及CD90分子,不表达CD34和CD45。AD-MSCs成脂能力优于UC-MSCs,但成骨能力没有明显差异。UC-MSCs抑制PBMC增殖能力优于AD-MSCs。 结论 UC-MSCs在免疫调节方面是一种更佳的种子细胞选择。

关键词: 脐带间充质干细胞, 脂肪源性间充质干细胞, 细胞增殖, 细胞分化, 免疫抑制

Abstract: Objective To study the biological characteristics of mesenchymal stem cells(MSCs)derived from human umbilical cord and adipose in vitro. Methods Umbilical cord-derived mesenchymal stem cells(UC-MSCs)and adipose-derived mesenchymal stem cells(AD-MSCs)were isolated. Cell growth curve was detected using CCK-8 method, and cell surface antigens were measured using flow cytometry. The adipogenic and osteogenic differentiation assays were analyzed by Oil Red O, Alizarin Red S and Von Kossa staining. The immunomodulatory properties of UC-MSCs and AD-MSCs on lymphocytes were evaluated through the co-culture assay with PHA activated adult peripheral blood monocuclear cells(PBMCs). Results Cell growth curve showed that UC-MSCs proliferated more rapidly than AD-MSCs. Flow cytometry analysis showed that UC-MSCs and AD-MSCs were positive for CD13、CD44、CD73 and CD90, and 山 东 大 学 学 报 (医 学 版)56卷3期 -黄平,等.脐带和脂肪源性间充质干细胞生物学特性比较 \=-negative for CD34 and CD45. AD-MSCs had the stronger adipogenetic ability than UC-MSCs, but there were no difference on osteogenetic ability between these two cells. UC-MSCs has the stronger suppression effects on PBMC proliferation than AD-MSCs. Conclusion UC-MSCs are the more attractive cell population for usage in immune cellular therapy.

Key words: Umbilical cord-derived mesenchymal stem cells, Adipose-derived mesenchymal stem cell, Cell proliferation, Cell differentiation, Immunosuppression

中图分类号: 

  • R329.2
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