RhoA/ROCK信号通路与原发性高血压患者血压变异性及颈动脉内膜中层厚度的相关性

doi:10.6040/j.issn.1671-7554.0.2014.768

摘要/Abstract

摘要： 目的探讨原发性高血压患者血清RhoA激酶(ROCK)水平与血压变异性(BPV)及颈动脉内膜中层厚度(IMT)的关系.方法选取2013年9月至2014年8月山东大学附属济南市中心医院心内科原发性高血压患者73例,根据血清ROCK1水平分为高表达组(n=37)和低表达组(n=36).选取同期体检血压正常者40例为正常对照组.采用Human Rock1 Elisa kit及酶标仪检测血清ROCK1水平;监测24 h血压,记录24 h、白昼、夜间血压均值以及相应的血压标准差;采用彩色多普勒超声仪测量颈动脉IMT.结果高表达组和低表达组血清ROCK1水平明显高于正常对照组(P<0.01);高表达组24 h收缩压标准差(24hSSD)、日间收缩压标准差(dSSD)、夜间收缩压标准差(nSSD)均明显高于低表达组(P<0.05);高表达组和低表达组颈动脉IMT比正常对照组明显增厚,高表达组颈动脉IMT比低表达组明显增厚(P<0.05);血清ROCK1水平与24hSSD、dSSD、nSSD及颈动脉IMT呈显著正相关性(P<0.05).结论高血压患者血压变异性增高与RhoA/ROCK1信号通路高表达有关,RhoA/ROCK1信号通路过度激活可导致颈动脉IMT增厚及颈动脉粥样硬化斑块(AS)形成.

关键词: 动态血压监测, 动脉粥样硬化, 高血压, RhoA/ROCK, 血压变异性, 内膜中层厚度

Abstract: Objective To investigate the association of the blood pressure variability (BPV) and carotid intima-media thickness (IMT) with the Rho/ROCK signaling pathway in essential hypertensive patients. Methods A total of 73 hypertensive patients were divided into two groups based on serum level of ROCK1: group with high level of ROCK1 (n=37) and group with low level of ROCK1 (n=36), and 40 healthy subjects were selected as the control group. The serum level of ROCK1 was measured with Human Rock1 Elisa kit and microplate reader. The mean blood pressures (systolic and diastolic) and the standard deviations of 24 h, daytime and nighttime (24hSSD, dSSD, nSSD, 24hDSD, dDSD and nDSD) were monitored. The carotid artery IMT was determined with color Doppler ultrasound monitor. Results The serum level of ROCK1 in hypertensive group was higher than that of the control group (P<0.05). Compared with the group with low ROCK1 level, the 24hSSD, dSSD and nSSD were significantly increased in the group with high ROCK1 level (P<0.05). The carotid artery IMT in hypertensive patients was thicker than that of control group (P<0.05). The carotid artery IMT of the group with high ROCK1 level was thicker than that of the group with low ROCK1 level (P<0.05). The serum level of ROCK1 showed significant positive correlations with 24hSSD, dSSD, nSSD and carotid artery IMT in hypertensive patients (P<0.05). Conclusion The BPV is closely correlated with expression of Rho/ROCK signaling pathway in hypertensive patients. Overexpression of Rho/ROCK signalingpathway plays a key role in the progression of carotid artery IMT and atherosclerosis.

Key words: RhoA/ROCK, Intima-media thickness, Hypertension, Blood pressure variability, Atherosclerosis, Ambulatory blood pressure monitoring

中图分类号:

R544.1

徐忠阳, 王立启, 徐振兴, 赵乾, 朱世明. RhoA/ROCK信号通路与原发性高血压患者血压变异性及颈动脉内膜中层厚度的相关性[J]. 山东大学学报（医学版）, 2015, 53(2): 48-51.

XU Zhongyang, WANG Liqi, XU Zhenxing, ZHAO Qian, ZHU Shiming. Correlations among RhoA/ROCK signaling pathway, blood pressure variability and carotid artery intima-media thickness in essential hypertensive patients[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 48-51.

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