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山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (6): 1-5.doi: 10.6040/j.issn.1671-7554.0.2018.109

• 基础医学 •    下一篇

穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用

苏晓慧1,孙明琪1,张梅洁1,方丽1,罗丹丹1,陈国平2,于春晓1,管庆波1   

  1. 1.山东大学附属省立医院内分泌科 山东省内分泌与脂代谢重点实验室 山东省临床医学研究院内分泌代谢研究所, 山东 济南 250021;2.北京穗加软件有限公司, 北京 100086
  • 发布日期:2022-09-27
  • 通讯作者: 管庆波. E-mail:doctorguanqingbo@163.com 于春晓. E-mail:yuchx08@163.com苏晓慧和孙明琪为共同第一作者.
  • 基金资助:
    国家自然科学基金(81471078、81770860)

Application of Suiplus semen analysis automatic detection system and manual counting using hemocytometer in mouse semen analysis

SU Xiaohui1, SUN Mingqi1, ZHANG Meijie1, FANG Li1, LUO Dandan1, CHEN Guoping2, YU Chunxiao1, GUAN Qingbo1   

  1. 1. Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University;
    Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism;
    Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan 250021, Shandong, China;
    2. Beijing Suiplus Software Co. Ltd, Beijing 100086, China
  • Published:2022-09-27

摘要: 目的 比较穗加精液分析(SSA)自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的功能及效果。 方法 采用SSA自动检测系统和血细胞计数板分别对成年雄性C57BL/6小鼠的精子一般形态、浓度、运动学参数及活力进行检测,并进行统计学分析。 结果 采用SSA自动检测系统计数小鼠的精子浓度显著高于血细胞计数板人工计数法(P<0.05)。SSA自动检测系统检测小鼠精子直线速率为(25.41±6.42)μm/s,曲线速率为(74.13±5.82)μm/s,直线性为(24.63±8.43)%,前向性为(41.00±9.62)%。小鼠的精子活动率SSA自动检测系统高于血细胞计数板人工计数法(P<0.05)。 结论 SSA自动检测系统能够直接观察精子的形态、运动并进行相应的参数分析,准确率高,操作简便,为小鼠精液的分析提供了方便可靠的方法。

关键词: 精液分析, 穗加精液分析自动检测系统, 血细胞计数板人工计数, 小鼠

Abstract: Objective To compare the functions and differences of Suiplus semen analysis(SSA)automatic detection system and manual counting using hemocytometer in mouse semen analysis. Methods The general morphology, concentration, kinematic parameters and motility of sperm samples of adult male C57BL/6 mice were measured by the above two methods respectively. The data were statistically analyzed. Results The sperm concentration detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). The results of the SSA system showed that the straight line velocity was(25.41±6.42)μm/s, the elocity in curvilinear velocity was(74.13±5.82)μm/s, the linearity was(24.63±8.43)%, and the straightness was(41.00±9.62)%. The proportion of motile sperm detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). Conclusion SSA automatic 山 东 大 学 学 报 (医 学 版)56卷6期 -苏晓慧,等.穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用 \=-detection system can directly observe the morphology and movement of sperm, and analyze the corresponding parameters simply but accurately, which provides a convenient and reliable method for semen analysis.

Key words: Semen analysis, Suiplus semen analysis automatic detection system, Manual counting using hemocytometer, Mouse

中图分类号: 

  • R588.1
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