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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (10): 6-10.doi: 10.6040/j.issn.1671-7554.0.2016.247

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Annexin A7在匹罗卡品癫痫小鼠模型脑组织中的表达

贾俊伟1,周雪颖2,李秋红1,于凯1,周国钰3,周盛年1   

  1. 1.山东大学齐鲁医院神经内科 山东大学脑科学研究所;2.山东大学齐鲁医院康复医学科; 3.山东大学齐鲁医院干部保健科, 山东 济南 250012
  • 收稿日期:2016-03-09 出版日期:2016-10-10 发布日期:2016-10-10
  • 通讯作者: 周盛年. E-mail:zhoushengnian126@126.com E-mail:zhoushengnian126@126.com
  • 基金资助:
    国家自然科学基金(81371439)

Expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model

JIA Junwei1, ZHOU Xueying2, LI Qiuhong1, YU Kai1, ZHOU Guoyu3, ZHOU Shengnian1   

  1. 1. Department of Neurology, Qilu Hospital of Shandong University &
    Brain Science Research Institute, Shandong University;
    2. Department of Rehabilitation Medicine, Qilu Hospital of Shandong University;
    3. Department of Cadre Health Care, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-03-09 Online:2016-10-10 Published:2016-10-10

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摘要： 目的 探讨Annexin A7在匹罗卡品癫痫小鼠模型脑组织中的表达。 方法 将30只健康成年雄性C57BL/6小鼠随机分为实验组(n=20)和对照组(n=10),实验组建立癫痫小鼠模型,观察两组行为学特点,分别运用蛋白质免疫印迹和免疫荧光标记技术检测Annexin A7在两组小鼠脑组织中的表达。 结果 实验组20只小鼠中有18只出现了癫痫持续状态,最终存活8只,其中有6只出现自发性发作,对照组均未出现癫痫持续状态和自发性发作。实验组海马和颞叶皮质中Annexin A7的表达均显著高于对照组(P<0.05);实验组海马和颞叶皮质中Annexin A7表达于神经元,而不表达于星形胶质细胞。 结论 Annexin A7可能参与癫痫的发生与发展。

关键词: 小鼠, 脑组织, Annexin A7, 癫痫, 匹罗卡品

Abstract: Objective To explore the expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model. Methods Thirty healthy adult male C57BL/6 mice were randomly divided into experimental group(n=20)and control group(n=10). Epilepsy mouse model was established in experimental group and behavioural characteristics of mice were observed in two groups. Expression of Annexin A7 was detected using Western blotting and immunofluorescence labeling techniques. Results Eighteen mice suffered from status epilepticus and only eight survived in the experimental group. Of which, six mice encountered spontaneous seizures in experimental group. Status epilepticus and spontaneous seizures did not occur in control group. Expression of Annexin A7 in the hippocampus and temporal cortex of experimental group were markedly higher than that of control group(P<0.05). In the brain tissue of epilepsy mouse model, Annexin A7 was expressed in the neurons rather than astrocytes. Conclusion Annexin A7 may be involved in the occurrence and development of epilepsy.

Key words: Epilepsy, Mouse, Brain tissue, Pilocarpine, Annexin A7

中图分类号: 

  • R742.1
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