脑源性神经营养因子在小鼠肠黏膜屏障中的作用

doi:10.6040/j.issn.1671-7554.0.2015.1340

摘要/Abstract

摘要： 目的运用基因敲除小鼠模型探讨脑源性神经营养因子(BDNF)在小鼠肠上皮屏障的作用。方法取BDNF^+/+小鼠与BDNF^+/-小鼠回肠及结肠黏膜,用透射电镜观察肠黏膜上皮的超微结构。免疫组织化学检测紧密连接蛋白ZO-1与occludin在结肠黏膜表达的变化,免疫印迹测定紧密连接蛋白claudin-1与claudin-2在结肠黏膜表达的差异。结果与BDNF^+/+小鼠相比,BDNF^+/-小鼠电镜下回肠上皮未见明显异常,结肠上皮屏障破坏,表现为微绒毛缺失、细胞间隙增宽以及细菌入侵;BDNF^+/-小鼠结肠上皮细胞紧密连接蛋白occludin、ZO-1以及claudin-1表达下调,而claudin-2表达上调。结论 BDNF可能在小鼠结肠上皮屏障功能的发挥中起到调控作用。

关键词: 紧密连接蛋白, 脑源性神经营养因子, 肠屏障, 小鼠

Abstract: Objective To investigate the effects of brain-derived neurotrophic factor(BDNF)on intestinal barrier integrity in mice. Methods Ileal tissues and colonic tissues from BDNF^+/+ mice and BDNF^+/- mice were prepared. The intestinal epithelial structure was detected by transmission electron microscopy. Expressions and distributions of tight junction proteins(occludin and ZO-1)in the colonic mucosa were evaluated by immunohistochemistry. Expressions of tight junction proteins(claudin-1 and claudin-2)in the colonic mucosa were evaluated by Western blotting. Results Compared with BDNF^+/+ mice, BDNF^+/- mice displayed integral ileal epithelial barrier and impaired colonic mucosa integrity which was characterized by diminished microvilli, widened intercellular space and bacteria invasion in the colonic mucosa. BDNF^+/- mice also exhibited increased expression of claudin-2 and decreased expression of occludin, ZO-1 and claudin-1 in the colonic mucosa. Conclusion BDNF may play a role in regulating the colonic epithelial barrier in mice.

Key words: Brain-derived neurotrophic factor, Intestinal barrier, Mice, Tight junction proteins

中图分类号:

R574

赵栋燕,于岩波,左秀丽. 脑源性神经营养因子在小鼠肠黏膜屏障中的作用[J]. 山东大学学报（医学版）, 2016, 54(7): 1-5.

ZHAO Dongyan, YU Yanbo, ZUO Xiuli. Brain-derived neurotrophic factor modulates intestinal barrier integrity in mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 1-5.

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