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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (9): 8-12.doi: 10.6040/j.issn.1671-7554.0.2015.219

• 基础医学 • 上一篇    下一篇

小鼠胚胎干细胞分泌因子对前列腺癌细胞作用的体外研究

翟红运1, 张登禄1, 王光杰1, 孔峰2, 程广辉2, 赵升田1   

  1. 1. 山东大学第二医院 泌尿外科, 山东 济南 250033;
    2. 山东大学第二医院 中心实验室, 山东 济南 250033
  • 收稿日期:2015-02-28 出版日期:2015-09-10 发布日期:2015-09-10
  • 通讯作者: 赵升田。E-mail:zhaoshengtian@sdu.edu.cn E-mail:zhaoshengtian@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81272815);泰山学者特聘教授科研基金

Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro

ZHAI Hongyun1, ZHANG Denglu1, WANG Guangjie1, KONG Feng2, CHENG Guanghui2, ZHAO Shengtian1   

  1. 1. Department of Urology, Jinan 250033, Shandong, China;
    2. Central Laboratory, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-02-28 Online:2015-09-10 Published:2015-09-10
  • Contact: 赵升田。E-mail:zhaoshengtian@sdu.edu.cn E-mail:zhaoshengtian@sdu.edu.cn

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摘要： 目的 研究小鼠胚胎干细胞(ESC)分泌因子对前列腺癌细胞RM-1生物学特性的影响,并探讨其作用机制。方法 采用Transwell小室在体外建立ESC联合小鼠胚胎成纤维细胞(MEF)与RM-1细胞的非接触式共培养体系作为实验干预(实验组),同时建立MEF与RM-1细胞的共培养体系作为阴性对照(对照组)。共培养72 h后,检测实验组与对照组RM-1细胞的增殖水平,比较两组细胞的形态学、细胞周期、凋亡水平、迁移与侵袭能力的差异。结果 与对照组比较,实验组RM-1细胞的形态表现为排布稀疏,分裂减少,死亡增多,部分细胞呈现凋亡迹象;实验组RM-1细胞增殖减慢(P<0.01),细胞周期存在G1→S期阻滞且凋亡增加,以晚期凋亡为主(P均<0.01);实验组RM-1细胞的迁移和侵袭能力减弱(P均<0.05)。结论 ESC分泌因子可通过阻滞RM-1细胞周期、促进其凋亡使RM-1细胞的增殖减慢,同时减弱其迁移与侵袭能力而发挥抗肿瘤作用。

关键词: 胚胎干细胞, 分泌因子, 前列腺癌细胞RM-1, 肿瘤, 细胞环境, 小鼠

Abstract: Objective To study the effects of factors secreted by mouse embryonic stem cells (ESC) on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts (MEF) and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability between the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group proliferated more slowly (P<0.01), with cell cycle arrest between phase G1 and S in addition with increased apoptosis, especially late stage apoptosis (both P<0.01). The RM-1 cells in experimental group showed weaker migration and invasion ability (both P<0.05). Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM-1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability.

Key words: Secreted factors, Prostate cancer cell RM-1, Cellular environment, Embryonic stem cell, Mouse, Neoplasia

中图分类号: 

  • R737.25
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