您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (4): 16-21.doi: 10.6040/j.issn.1671-7554.0.2014.965

• 基础医学 • 上一篇    下一篇

miR-584对人胶质母细胞瘤侵袭和迁移能力的影响

韩晓, 薛皓, 闫绍峰, 郭兴, 李彤, 郭小凡, 高校, 李刚   

  1. 山东大学齐鲁医院神经外科, 山东 济南 250012
  • 收稿日期:2014-12-17 修回日期:2015-02-23 出版日期:2015-04-10 发布日期:2015-04-10
  • 通讯作者: 李刚.E-mail:doctorligang@126.com E-mail:doctorligang@126.com
  • 基金资助:
    国家自然科学基金(81372719);国家自然科学基金青年基金(81101594)

Effects of miR-584 on the invasion and migration of human glioblastoma U251 cells

HAN Xiao, XUE Hao, YAN Shaofeng, GUO Xing, LI Tong, GUO Xiaofan, GAO Xiao, LI Gang   

  1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-17 Revised:2015-02-23 Online:2015-04-10 Published:2015-04-10

PDF (PC)

127

摘要： 目的 探讨miR-584对人胶质母细胞瘤U251细胞侵袭和迁移能力的影响.方法 将化学合成的miR-584-3p在脂质体Lipofectamine 2000的介导下,瞬时转染人胶质母细胞瘤U251,细胞分为空白对照组(无转染,其他条件相同)、mimics NC组(转染miR-584-3p mimics阴性对照序列)、miR-584-3p mimics组(转染miR-584-3p mimics)、inhibitor NC组(转染miR-584-3p inhibitor阴性对照序列)和miR-584-3p inhibitor组(转染miR-584-3p inhibitor).Real-time PCR法检测细胞中miR-584-3p的表达水平;CCK-8法检测细胞增殖能力;划痕实验和Transwell小室实验检测细胞的迁移和侵袭能力.结果 与空白对照组和mimics NC组相比,miR-584-3p mimics组的miR-584-3p表达上调约100倍(P <0.05), 细胞增殖能力无明显差异(P >0.05),细胞侵袭和迁移能力明显下降(P <0.05); 与空白对照组和inhibitor NC组相比,miR-584-3p inhibitor组的miR-584-3p表达下调至空白对照组的5%(P <0.05), 细胞增殖能力无明显差异(P >0.05),细胞侵袭和迁移能力显著增强(P <0.05).结论 miR-584-3p mimics转染抑制了U251细胞的侵袭和迁移能力; miR-584-3p inhibitor转染能增强U251细胞的侵袭和迁移能力.

关键词: 胶质母细胞瘤, miR-584, 迁移, 侵袭

Abstract: Objective To explore the effect of miR-584 expression on the invasion and migration of glioblastoma U251 cells in vitro. Methods Chemosynthetic miR-584-3p was transfected into glioblastoma cells under the induction of Lipofectamine 2000. The cells were divided into control group(no transfection), mimics NC group(transfected with negative control sequence of miR-584-3p mimics), miR-584-3p mimics group(transfected with miR-584-3p mimics), inhibitor NC group(transfected with negative control sequence of miR-584-3p inhibitor), miR-584-3p inhibitor group(transfected with miR-584-3p inhibitor).The expression of miR-584-3p was detected by Real-time PCR. Cell proliferation was detected by CCK-8 proliferation assay. The invasive and migratory effects of glioblastoma cell were measured by Wound healing and Transwell assays. Results Compared with mimics NC group and control group, the expression of miR-584-3p in miR-584-3p mimics group was upregulated by 100 times(P< 0.05). Compared with mimics NC group and control group, the cell proliferation ability of miR-584-3p mimics group showed no significant change(P >0.05), but the cellular migratory and invasive activity of miR-584-3p mimics group were down-regulated significantly(P <0.05). Compared with inhibitor NC group and control group, the expression of miR-584-3p in miR-584-3p inhibitor group was down-regulated by 20 times(P <0.05). Compared with inhibitor NC group and control group, the cell proliferation ability of miR-584-3p inhibitor group showed no significant change(P >0.05), the cellular migration and invasive activity in miR-584-3p inhibitor group were up-regulated significantly(P <0.05). Conclusion The migration and invasion of glioblastoma cells can be inhibited by the over-expression of miR-584-3p, while the transfection of miR-584-3p inhibitor can enhance the migration and invasion of glioblastoma cells.

Key words: MiR-584, Glioblastoma, Invasion, Migration

中图分类号: 

  • R739.41
[1] Wen PY, Kesari S. Malignant gliomas in adults[J]. N Engl J Med, 2008, 359(17):492-507.
[2] 尹丰, 张剑宁, 赵明明, 等.胶质瘤干细胞与神经干细胞基因表达差异的微阵列基因芯片分析[J].解放军医学杂志, 2014, 39(10):800-803. YIN Feng, ZHANG Jianning, ZHAO Mingming, et al.Identification of the difference in gene expression between glioma stem cells and neural stem cells by oligonucleotide microarray[J].Med J Chin PLA, 2014, 39(10):800-803.
[3] Feng W, Feng Y. MicroRNAs in neural cell development and brain diseases[J]. Sci China Life Sci, 2011, 54(12):1103-1112.
[4] Kefas B, Godlewski J, Comeau L, et al. MicroRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma[J]. Cancer Res, 2008, 68(10):3566-3572.
[5] Sasayama T, Nishihara M, Kondoh T, et al. MicroRNA-10b is overexpressed in malignant glioma and associated with tumor invasive factors, uPAR and RhoC[J]. Int J Cancer, 2009, 125(6):1407-1413.
[6] Xia H, Qi Y, Ng SS, et al. MicroRNA-146b inhibits glioma cell migration and invasion by targeting MMPs[J]. Brain Res, 2009, 126(9):158-165.
[7] Landgraf P, Rusu M, Sheridan R, et al.A mammalian microRNA expression atlas based on small RNA library sequencing[J]. Cell, 2007, 29(7):1401-1414.
[8] 徐丽平,李佳,芳林,等. 微小RNA-518b在乳腺浸润性导管癌中的表达及其功能[J]. 中华实验外科杂志,2012, 29(5):833-836. XU Liping, LI Jia, FANG Lin, et al.Expression and function of microRNA-518b in invasive ductal carcinoma of breast[J]. Chin J Exp Surg, 2012, 29(5):833-836.
[9] 王进,周建萍,杨巧媛,等.微小RNA-98在胃癌中表达下调及其对胃癌生长的调控作用[J].中华实验外科杂志, 2013, 30(6):1245-1247. WANG Jin, ZHOU Jianping, YANG Qiaoyuan, et al.Low expression of microRNA-98 in gastric cancer and the inhibitory effect on gastric cell growth[J].Chin J Exp Surg, 2013, 30(6):1245-1247.
[10] Mertens-Talcott SU, Chintharlapalli S, Li X, et al.The oncogenic microRNA-27a targets genes that regulate specificity protein transcription factors and the G2-M checkpoint in MDA-MB-231 breast cancer cells[J]. Cancer Res, 2007, 67(22):11001-11011.
[11] Ueno K, Hirata H, Shahryari V, et al. Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases invasion ability in human clear cell renal cell carcinoma[J]. Br J Cancer, 2011, 104(2):308-315.
[12] Nadege FA, Meiou D, Guo J, et al.MicroRNA-584 and the protein phosphatase and actin regulator 1 (PHACTR1), a new signaling route through which transforming growth factor-mediates the migration and actin dynamics of breast cancer cell[J]. J Biol Chem, 2013, 288(17):11807-11823.
[13] Hendrix D, Levine M, Shi W. miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data[J]. Genome Biol, 2010, 11(4):R39. doi:10.1186/gb-2010-11-4-r39.
[14] 夏大勇,徐善水.MMPs与EMMPRIN 在人脑胶质瘤侵袭性生长中研究的进展[J]. 临床神经外科杂志,2011,2(8):108-110. XIA Dayong, XU Shanshui.The research development of MMPs and EMMPRIN on invasive growth of human gliomas[J]. J Clin Neurosurgery, 2011,2(8):108-110.
[15] Yong VW. Metalloproteinases:mediators of pathology and regeneration in the CNS[J]. Nat Rev Neurosci, 2005, 6(12):931-944.
[16] Loftus JC, Ross JT, Paquette KM, et al.miRNA expression profiling in migrating glioblastoma cells:regulation of cell migration and invasion by miR-23b via targeting of Pyk2[J]. PLoS One, 2012, 7(6):398-418.
[1] 孙薏丰,高玉,梁永媛,高杨. CPLX2在30例肝癌组织的表达及其对体外细胞增殖与侵袭的影响[J]. 山东大学学报 (医学版), 2020, 1(9): 34-39.
[2] 王剑,周文婧,薛知易,刘晓菲. 脑胶质母细胞瘤模型研究概况及类脑模型的研发应用[J]. 山东大学学报 (医学版), 2020, 1(8): 74-80.
[3] 马青源,蒲沛东,韩飞,王超,朱洲均,王维山,史晨辉. miR-27b-3p调控SMAD1对骨肉瘤细胞增殖、迁移和侵袭作用的影响[J]. 山东大学学报 (医学版), 2020, 1(7): 32-37.
[4] 李宁,李娟,谢艳,李培龙,王允山,杜鲁涛,王传新. 长链非编码RNA AL109955.1在80例结直肠癌组织中的表达及对细胞增殖与迁移侵袭的影响[J]. 山东大学学报 (医学版), 2020, 1(7): 38-46.
[5] 王甜甜,陈洁. EFEMP2在子宫内膜癌组织及细胞中的表达及其临床意义[J]. 山东大学学报（医学版）, 2017, 55(10): 52-58.
[6] 陆衡,刘延国,李曙光,陈晓康,田琦,衣翠华,王秀问. YKL-40对卵巢癌SKOV-3细胞迁移能力的影响[J]. 山东大学学报（医学版）, 2017, 55(1): 33-38.
[7] 王伟,曹煜姗,孙达权,黄小琼,徐国强. 人TIMP-2蛋白在肝癌细胞迁移与侵袭中的作用[J]. 山东大学学报（医学版）, 2016, 54(7): 11-17.
[8] 贺武斌,苏荣健. 白头翁皂苷D联合索拉非尼对人肝癌细胞侵袭与转移的影响[J]. 山东大学学报（医学版）, 2016, 54(7): 18-22.
[9] 马雪,张斌,韩春耀,刘明媛,郝丽静,葛树卿,薛中原. 下调α-catulin基因的表达对舌鳞癌细胞株Tscca侵袭及迁移能力影响的体外研究[J]. 山东大学学报（医学版）, 2016, 54(6): 12-15.
[10] 郑荟,魏光伟. HIPK2抑制非小细胞肺癌上皮间质转化及迁移侵袭的作用及机制[J]. 山东大学学报（医学版）, 2016, 54(11): 7-12.
[11] 程翔宇, 邢锐, 邢召全, 郭兆新, 郭晓宇, 苏静, 孟力维, 刘照旭. 氯化两面针碱对前列腺癌细胞PC-3增殖与凋亡的影响[J]. 山东大学学报（医学版）, 2015, 53(9): 13-18.
[12] 吕树卿, 尉蔚, 张灿灿, 田永杰. HGF/PARP-1信号通路调控卵巢癌细胞侵袭转移的作用[J]. 山东大学学报（医学版）, 2015, 53(7): 1-7.
[13] 周静, 常晓天, 周婷, 崔莹莹, 张蓓, 荣风年. 沉默PADI4基因对卵巢癌细胞系OVCAR3的作用[J]. 山东大学学报（医学版）, 2015, 53(6): 48-53.
[14] 姚众, 陈为亮, 徐洋洋, 何影, 曲迅, 李新钢. 半乳凝素-9抑制胶质瘤细胞增殖及迁移[J]. 山东大学学报（医学版）, 2015, 53(4): 1-5.
[15] 高峰, 康白, 杨洪鸣, 赵廷坤, 曲梅花, 王金红. 皮肤T细胞淋巴瘤相关抗原5在肿瘤细胞株中的表达[J]. 山东大学学报（医学版）, 2015, 53(3): 41-45.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发