皮肤T细胞淋巴瘤相关抗原5在肿瘤细胞株中的表达

doi:10.6040/j.issn.1671-7554.0.2014.618

摘要/Abstract

摘要： 目的探讨皮肤T细胞淋巴瘤相关抗原5(cTAGE5)在肿瘤细胞株中的表达及其在肿瘤发生发展中的生物学作用。方法细胞免疫荧光化学检测cTAGE5来源于人的肿瘤细胞株的表达。取阳性表达cTAGE5的人卵巢透明细胞癌ES-2细胞, 分为正常对照组、脂质体对照组、siRNA转染组、表柔比星(终浓度1×10^-6mol/L)对照组,平板克隆形成试验和MTT法观察cTAGE5对细胞生长增殖能力的影响, 细胞划痕实验检测细胞迁移能力。结果 cTAGE5在乳腺癌、肺癌、卵巢癌均有表达, 而在胃癌和肝癌肿瘤细胞中表达不明显。与正常对照组比较, siRNA转染组的肿瘤细胞增殖生长抑制(P<0.01), 细胞迁移能力降低(P<0.01), 表柔比星对照组也显示肿瘤细胞增殖抑制(P<0.01), 细胞迁移能力降低(P>0.01)。结论 cTAGE5在不同组织来源的肿瘤细胞株表达存在差异, siRNA干扰cTAGE5表达可有效抑制肿瘤细胞的增殖和迁移能力。

关键词: 迁移, 皮肤T细胞淋巴瘤相关抗原5, 肿瘤细胞, RNA干扰, 增殖

Abstract: Objective To investigate the expression of the cutaneous T-cell lymphoma-associated antigen 5(cTAGE5) in tumor cell lines and its biological role in the development of tumor preliminarily. Methods Expression of cTAGE5 in tumor cell lines was detected by immunofluorescence. Ovarian clear cell adenocarcinoma cell line ES-2, which was proved to be positive expression of cTAGE5, were divided into normal control group, liposome control group, siRNA transfection group and epirubicin control group(final concentration 1×10^-6mol/L). The Proliferation and growth ability of ES-2 were detected by plate clone formation test and MTT test respectively, and migration ability was detected by cell scratch assay. Results cTAGE5 was expressed in cell lines got from human breast cancer, lung cancer and ovarian cancer, but little in cell lines from human stomach and liver cancer. Compared with normal control group, growth and proliferation ability of tumor cells in siRNA transfection group decreased(P<0.01), migration ability reduced (P<0.01), and proliferation ability of tumor cells in epirubicin control group decreased(P<0.01), also migration ability reduced (P<0.01). Conclusion Expression of cTAGE5 in tumor cell lines from different tissue was different. Inhibition of cTAGE5 expression can depress the proliferation, growth and migration ability of tumor cells.

Key words: Migration, Cutaneous T-cell lymphoma-associated antigen 5, Tumor cells, Proliferation, RNA interfering

中图分类号:

R966

高峰, 康白, 杨洪鸣, 赵廷坤, 曲梅花, 王金红. 皮肤T细胞淋巴瘤相关抗原5在肿瘤细胞株中的表达[J]. 山东大学学报（医学版）, 2015, 53(3): 41-45.

GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong. Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 41-45.

参考文献

[1] 严月华, 徐丽, 吴剑波, 等.白藜芦醇对人皮肤T细胞淋巴瘤Hut-78细胞增殖和凋亡影响观察[J]. 中华肿瘤防治杂志, 2013,20(23):1807-1811. YAN Yuehua, XU Li, WU Jianbo, et al. Effect of resveratrol on inducing proliferation and apoptosis of T cell line Hut-78 cells[J]. Chinese Journal of Cancer Prevention and Treatment, 2013, 20(23):1807-1811.
[2] Usener D, Schadendorf D, Koch J, et al. cTAGE:a cutaneous T cell lymphoma associated antigen family with tumor-specific splicing[J]. J Invest Dermatol, 2003, 121(1):198-206.
[3] Eichmuller S, Usener D, Dummer R, et al. Serological detection of cutaneous T-cell lymphoma-associated antigens[J]. Proc Natl Acad Sci U S A, 2001, 98(2):629-634.
[4] Comtesse N, Reus K, Meese E. The MGEA6 multigene family has an active locus on14q and at least nine pseudogenes no different chromosomes[J]. Genomics, 2001, 75(1-3):43-48.
[5] 陈万青, 张思维, 曾洪梅, 等.中国2010年恶性肿瘤发病与死亡[J].中国肿瘤, 2014,23(1):1-10. CHEN Wanqing, ZHANG Siwei, ZENG Hongmei, et al. Report of Cancer Incidence and Mortality in China,2010[J]. China Cancer, 2014, 23(1):1-10.
[6] 姜云霞, 王冰, 崔韶晖, 等.阳离子脂质体基因载体的细胞转染研究[J]. 安徽农业科学, 2009,37(17):7862-7864. JANG Yunxia, WANG Bing, CUI Shaohui, at al. Study on the Cell Transfection of the Cationic Liposome Gene Carrier[J]. Journal of Anhui Agricultural Sciences, 2009, 37(17):7862-7864.
[7] 王博龙.乌索酸对人肝星状细胞凋亡及TGFβ_1 mRNA表达的影响[J].中国药理学通报, 2010,26(3):417-418. WANG Bolong. Effect of ursolic on apoptosis and expression of TGFβ_1 mRNA in human heportic stellate cells[J]. Chinese Phamacological Bulletion, 2010, 26(3):417-418.
[8] 王苗苗, 刁雪芹, 任桂杰, 等.嘌呤霉素敏感的氨肽酶对Tau^p301L诱导细胞凋亡的影响[J].山东大学学报:医学版, 2014,52(4):1-7. WANG Miaomiao, DIAO Xueqin, REN Guijie, et al. Influence of puromycin-sensitive aminopeptidase on apoptosis induced by Tau^p301L in cells[J]. Journal of Shandong University:Health Sciences, 2014, 52(4):1-7.
[9] 张艳丽, 刘新风, 王传新, 等.循环miR-128在结直肠癌患者血清中的表达及其对细胞迁移侵袭能力的影响[J].山东大学学报:医学版, 2014,52(8):57-62. ZHANG Yanli, LIU Xinfeng, WANG Chuanxin, et al. Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells[J]. Journal of Shandong University:Health Sciences, 2014, 52(8):57-62.
[10] Comtesse N, Niedermayer I, Glass B, et al. MGEA6 is tumor specific overexpressed and frequently recognized by patient serum antibodies[J]. Oncogene, 2002, 21(2):239-247.
[11] Boon T, Old LJ. Cancer tumor antigens[J]. Curr Opin. Immunol, 1997, 9(5):681-683.
[12] 鹿蓉, 姚振威.分子探针在疾病诊疗中的应用与展望[J].上海医药, 2014,35(13):16-22. LU Rong, YAO Zhenwei. Exploration and prospect of molecular probes for disease diagnosis and treatment[J]. Shanghai Medical & Pharmaceutical Journal, 2014, 35(13):16-22.
[13] Kaiser J. A detailed genetic portrait of the deadliest human cancers[J]. Science, 2008, 321(5894):1280-1281.
[14] Lemos RR, Oliveira DF, Zatz M, et al. Population and computational analysisof the MGEA6 P521A variation as a risk factor for familial idiopathic basal ganglia calcification (Fahr's disease)[J]. J Mol Neurosci, 2011, 43(3):333-336.
[15] Saliminejad K , Ashtari F, Kamali K. Analysis of the CTAGE5 P521A Variation with the Risk of Familial Idiopathic Basal Ganglia Calcification in an Iranian Popula[J]. J Mol Neurosci, 2013, 49(3):614-617.
[16] Oliveira JR, Sobrido MJ, Spiteri E, et al. Analysis of candidate genes at theIBGC1 locus associated with idiopathic basal ganglia calcification (“Fahr's disease”)[J]. J Mol Neurosci, 2007, 33(2):151-154.
[17] Brodaty H, Mitchell P, Luscombe G, et al. Familial idiopathic basal ganglia calcification (Fahr's disease) without neurological, cognitive and psychiatric symptoms is not linked to the IBGC1 locus on chromosome 14q[J]. Hum Genet, 2002,110(1):8-14.
[18] Bacarizo J, Camara-Artigas A. Atomic resolution structures of the c-Src SH3 domain in complex with two high-affinity peptides from classes I and II[J]. Acta Crystallogr D Biol Crystallogr, 2013, 69(5):756-766.
[19] Ren S, Peng Z, Mao JH, et al. RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings[J]. Cell Res, 2012, 22(5):806-821.
[20] 王丽, 刘晓丽, 李智慧, 等. ITSN1-S的SH3功能域对恶性胶质瘤细胞U87增殖能力的影响[J]. 中国肿瘤临床, 2013, 40(18):1089-1093. WANG Li, LIU Xiaoli, LI Zhihui, et al. ITSN1-S SH3 domains regulate human glioblastoma U87 cell proliferation[J]. Chinese Journal of Clinical Oncology, 2013, 40(18):1089-1093.

相关文章 15

[1]	王晓磊张海涛张辉郭成浩. 舒血宁注射液对高碘致培养血管内皮细胞损伤的保护作用[J]. 山东大学学报(医学版), 2209, 47(6): 38-.
[2]	李涵,付婷婷,张磊,延冰,孙涛,郭峰,尹晓. 过氧化物酶增殖物激活受体γ激动剂对24例肥胖症患者米色脂肪细胞分化的影响[J]. 山东大学学报 (医学版), 2020, 1(9): 8-13.
[3]	孙薏丰,高玉,梁永媛,高杨. CPLX2在30例肝癌组织的表达及其对体外细胞增殖与侵袭的影响[J]. 山东大学学报 (医学版), 2020, 1(9): 34-39.
[4]	张宝文,雷香丽,李瑾娜,罗湘俊,邹容. miR-21-5p靶向调控TIMP3抑制2型糖尿病肾病小鼠肾脏系膜细胞增殖及细胞外基质堆积[J]. 山东大学学报 (医学版), 2020, 1(7): 7-14.
[5]	付洁琦,张曼,张晓璐,李卉,陈红. Toll样受体4抑制过氧化物酶体增殖物激活受体γ加重血脂蓄积的分子机制[J]. 山东大学学报 (医学版), 2020, 1(7): 24-31.
[6]	马青源,蒲沛东,韩飞,王超,朱洲均,王维山,史晨辉. miR-27b-3p调控SMAD1对骨肉瘤细胞增殖、迁移和侵袭作用的影响[J]. 山东大学学报 (医学版), 2020, 1(7): 32-37.
[7]	李宁,李娟,谢艳,李培龙,王允山,杜鲁涛,王传新. 长链非编码RNA AL109955.1在80例结直肠癌组织中的表达及对细胞增殖与迁移侵袭的影响[J]. 山东大学学报 (医学版), 2020, 1(7): 38-46.
[8]	王凯民,谭娟娟,严志强,李志强. Sirtuin在低氧诱导人脐带间充质干细胞增殖中的作用[J]. 山东大学学报（医学版）, 2017, 55(7): 23-30.
[9]	史培堃,曾贝妮,吴伟芳,胡晓燕,马天加,周亚滨. Keap1在肾细胞癌中的表达及其作用[J]. 山东大学学报（医学版）, 2017, 55(3): 94-99.
[10]	孙泽雨,陈颖,林家香,刘娟,赵蔚明. 磷脂酰肌醇-4-磷酸酶II型对宫颈癌细胞增殖及凋亡的影响[J]. 山东大学学报（医学版）, 2017, 55(11): 1-6.
[11]	王甜甜,陈洁. EFEMP2在子宫内膜癌组织及细胞中的表达及其临床意义[J]. 山东大学学报（医学版）, 2017, 55(10): 52-58.
[12]	陆衡,刘延国,李曙光,陈晓康,田琦,衣翠华,王秀问. YKL-40对卵巢癌SKOV-3细胞迁移能力的影响[J]. 山东大学学报（医学版）, 2017, 55(1): 33-38.
[13]	赵路,矫俊,张腾,焦薪霖,崔保霞. 肝X受体在卵巢癌组织中的表达及T0901317在卵巢癌细胞系SKOV3中的作用[J]. 山东大学学报（医学版）, 2017, 55(1): 49-53.
[14]	田斯琦,刘日强,杨宁,韦薇,杨华伟. 核糖体S6蛋白激酶4变异体基因表达对乳腺癌细胞增殖的影响[J]. 山东大学学报（医学版）, 2016, 54(9): 32-36.
[15]	马雪,张斌,韩春耀,刘明媛,郝丽静,葛树卿,薛中原. 下调α-catulin基因的表达对舌鳞癌细胞株Tscca侵袭及迁移能力影响的体外研究[J]. 山东大学学报（医学版）, 2016, 54(6): 12-15.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed