EFEMP2在子宫内膜癌组织及细胞中的表达及其临床意义

doi:10.6040/j.issn.1671-7554.0.2017.049

摘要/Abstract

摘要： 目的探讨包含表皮生长因子的fibulin类细胞外基质2(EFEMP2)在子宫内膜癌组织以及子宫内膜癌细胞中的表达及其临床意义。方法用免疫组织化学法检测200例子宫内膜癌组织以及40例正常子宫内膜组织中EFEMP2的表达;采用免疫细胞化学法、qRT-PCR、Western blotting检测EFEMP2在4种子宫内膜癌细胞(KLE、HEC-1A、HEC-1B、Ishikawa)中的表达水平;运用生长曲线及软琼脂克隆形成实验检测子宫内膜癌细胞的增殖能力;运用Transwell体外侵袭及转移实验检测子宫内膜癌细胞的侵袭及转移能力。结果 EFEMP2在子宫内膜癌组织、细胞中的表达明显低于正常子宫内膜组织及细胞(P<0.001),且与子宫内膜癌晚期、分化程度低、淋巴结转移密切相关(P<0.05)。KLE和HEC-1A的增殖、侵袭、转移能力高于HEC-1B和Ishikawa(P<0.001)。结论 EFEMP2 的表达与子宫内膜癌临床分期、组织分化程度、有无淋巴结转移和侵袭生长等恶性表型密切相关,并可能参与抑制子宫内膜癌侵袭、转移过程。

关键词: EFEMP2, 增殖, 转移, 子宫内膜癌, 侵袭

Abstract: Objective To investigate expression and the clinical significance of EGF containing fibulin-like extracellular matrix protein 2(EFEMP2)in endometrial cancer tissues and cell lines. Methods Immunohistochemical staining was performed to determine EFEMP2 expression in 200 endometrial carcinoma specimens and 40 normal endometrial tissue specimens. qRT-PCR, Western blotting and immunocytochemistry were performed to investigate EFEMP2 expression in normal endometrial cells compared with four kinds of endometrial carcinoma cell lines including KLE、HEC-1A、HEC-1B and Ishikawa. Growth curve assay and soft agar colony formation assay were used to investigate the proliferation of endometrial cancer cell lines. Transwell assays were used to analyze the invasion and migration of endometrial cancer cell lines. Results EFEMP2 was down-regulated in endometrial cancer tissues and cells compared with normal endometrial tissues and cells(P<0.001), respectively. EFEMP2 low expression was significantly related with high stage, low differentiation and positive lymph node status of endometrial cancer(P<0.05). KLE and HEC-1A cells had higher proliferation, invasion and metastasis abilities compared to HEC-1B and Ishikawa cells(P<0.001). Conclusion EFEMP2 expression was significantly related with tumor stage, tissue differentiation and lymph node status of endometrial cancer. It is possible that EFEMP2 inhibits endometrial cancer cell invasion and migration.

Key words: Proliferation, Invasion, EFEMP2, Endometrial cancer, Migration

中图分类号:

R574

王甜甜,陈洁. EFEMP2在子宫内膜癌组织及细胞中的表达及其临床意义[J]. 山东大学学报（医学版）, 2017, 55(10): 52-58.

WANG Tiantian, CHEN Jie. Expression and clinical significance of EFEMP2 in endometrial cancer tissues and cell[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 52-58.

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