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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (1): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.359

• 基础医学 • 上一篇    下一篇

miRNA-486-5p对结肠癌细胞株SW620生物学行为的影响

刘成霞1, 李明2, 连海峰1, 史宁1   

  1. 1. 滨州医学院附属医院消化内科, 山东 滨州 256603;
    2. 滨州医学院烟台附属医院消化内科, 山东 烟台 264100
  • 收稿日期:2014-06-03 修回日期:2014-12-17 发布日期:2015-01-10
  • 通讯作者: 连海峰。E-mail:lianhaifeng01@163.com E-mail:lianhaifeng01@163.com
  • 基金资助:
    山东省自然科学基金(2010ZRB14177)

Impact of miR-486-5p on the biological behavior of colorectal carcinoma SW620 cells

LIU Chengxia1, LI Ming2, LIAN Haifeng1, SHI Ning1   

  1. 1. Department of Gastroenterology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong, China;
    2. Department of Gastroenterology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China
  • Received:2014-06-03 Revised:2014-12-17 Published:2015-01-10

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摘要： 目的 探讨微小RNA-486-5p(miRNA-486-5p)对结肠癌细胞SW620增殖、凋亡及迁移能力的影响。方法 构建miRNA-486-5p过表达质粒,采用脂质体法瞬时转染结肠癌细胞株SW620,实时定量PCR(qRT-PCR)检测转染细胞后miRNA-486-5p的表达丰度,MTT法及流式细胞仪检测细胞的增殖及凋亡情况,Transwell小室迁移实验检测细胞的迁移能力。结果 SW620转染miRNA-486-5p过表达质粒后,miRNA-486-5p表达明显上调(8.72±0.52 vs 1.02±0.06),细胞增殖能力降低(0.92±0.02 vs 1.40±0.03)、凋亡率增高[(15.30±2.60)% vs (4.70±1.30)%],迁移能力降低(75.20±10.60 vs 142.50±11.30),差异均有统计学意义(P<0.05)。结论 miRNA-486-5p可抑制结肠癌细胞SW620的增殖和迁移能力,促进其凋亡,有望成为结肠癌治疗的新靶点。

关键词: 结直肠癌, miRNA-486-5p, 凋亡, 增殖, 迁移, 转染

Abstract: Objective To investigate the effect of microRNA-486-5p (miR-486-5p) on the proliferation, apoptosis and migration of human colorectal cancer cell line SW620. Methods The miR-486-5p over-expressing plasmid was constructed and transfected into human colorectal carcinoma cell line SW620 using LipofectamineTM2000. The expression of miR-486-5p of the transfected cells was measured with quantitative real-time PCR (qRT-PCR), the proliferation and apoptosis of SW620 cells were assessed with MTT and flow cytometry, and the in vitro migration abilities of SW620 cells was detected with Transwell test. Results The expression of miR-486-5p in SW620 cells was markedly up-regulated (8.72±0.52 vs 1.02±0.06). The proliferation (0.92±0.02 vs 1.40±0.03) and migration abilities of SW620 cells (75.20±10.60 vs 142.50±11.30)were inhibited significantly, and apoptosis rate[(15.30%±2.60)% vs (4.70±1.30)%] was increased (P<0.05). Conclusion MiR-486-5p can effectively suppress the proliferation and in vitro migration abilities of SW620 cells, and increase the apoptosis rate, indicating that miR-486-5p might be used as a target for molecular therapy of colorectal cancer.

Key words: Colorectal carcinoma, Apoptosis, MiR-486-5p, Transfection, Proliferation, Migration

中图分类号: 

  • R574
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