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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (2): 68-73.doi: 10.6040/j.issn.1671-7554.0.2015.486

• 基础医学 • 上一篇    下一篇

芳姜黄酮衍生物对WM35细胞增殖及凋亡的影响

涂云华1,陈利远1,王喜1,周英2,康颖倩3,薛月萃4,荣冬芸4,叶振源4,曹煜4   

  1. 1. 贵阳市第二人民医院皮肤科, 贵州 贵阳 550081;2.贵州大学生命科学学院贵州省中药民族药创制工程中心, 贵州 贵阳 550025;3. 贵州医科大学微生物学教研室, 贵州 贵阳 550004;4. 贵州医科大学附属医院皮肤性病科, 贵州 贵阳 550004
  • 收稿日期:2015-05-14 出版日期:2017-02-10 发布日期:2017-02-10
  • 通讯作者: 曹煜. E-mail:382541077@qq.com陈利远. E-mail:pfkcLy@163.com E-mail:382541077@qq.com
  • 基金资助:
    贵州省中药现代化专项项目［黔科合中药字(2012)5018号］;贵阳市科技局科技创新平台项目［筑科合同(2012303)号］;贵州省中药民族药创制工程中心专项项目［黔教合字(2012)018号］

Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells

TU Yunhua1, CHEN Liyuan1, WANG Xi1, ZHOU Ying2, KANG Yingqian3, XUE Yuecui4, RONG Dongyun4, YE Zhenyuan4, CAO Yu4   

  1. 1. Department of Dermatology, the Second Peoples Hospital of Guiyang City, Guiyang 550081, Guizhou, China;
    2. Guizhou Province Chinese Medicine and Ethnic Medicine Creation Engineering Center, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China;
    3. Department of Microbiology, Guizhou Medical University, Guiyang 550004, Guizhou, China;
    4. Department of Dermatology and Venereal Disease, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2015-05-14 Online:2017-02-10 Published:2017-02-10

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摘要： 目的 研究芳姜黄酮衍生物(ATD)对人皮肤黑色素瘤WM35细胞增殖及凋亡的影响,并探讨其作用机制。 方法 不同浓度(5~80 μmol/L)ATD体外作用WM35细胞。CCK-8法检测增殖抑制率;AO/EB染色、倒置显微镜观察细胞凋亡形态;DNA片段化检测细胞凋亡;比色法检测Caspase-3酶活性;流式细胞术检测细胞凋亡;Western blotting检测Bax及Bcl-2蛋白表达。 结果 ATD对WM35细胞有增殖抑制作用,呈时间-剂量依赖性(P<0.05)。ATD诱导WM35细胞凋亡,呈剂量依赖性(P<0.05),Caspase-3酶活性随药物浓度增加而增强(P<0.05)。随药物浓度增加,Bax/Bcl-2比值逐渐升高。 结论 ATD对WM35细胞有抑制增殖及促凋亡作用,其机制是使凋亡相关蛋白Bax表达上调、Bcl-2表达下调,激活细胞凋亡途径关键酶Caspase-3,进而抑制肿瘤细胞分化与增殖。

关键词: 芳姜黄酮衍生物, WM35细胞, 凋亡

Abstract: Objective To investigate the effect of ar-turmerone derivatives(ATD)on proliferation and apoptosis in human melanoma WM35 cells, and to explore its mechanism. Methods WM35 cells were incubated with different concentrations of ATD(5-80 μmol/L)in vitro. Cell proliferation was measured by cell counting kits(CCK-8)assay. The cell morphology of WM35 was observed by inverted microscope after AO/EB staining. Apoptosis was detected by DNA fragmentation. Caspase-3 cellular activities were measured by a colorimetric method. The apoptosis rate was analyzed by flow cytometry. The expression of apoptosis associated protein, Bcl-2 and Bax, in WM35 cells were examined by 山 东 大 学 学 报 (医 学 版)55卷2期 -涂云华,等.芳姜黄酮衍生物对WM35细胞增殖及凋亡的影响 \=-Western blotting. Results ATD exhibited obvious proliferation inhibition effect on the growth of WM35 cells in a time-and-dose dependent manner(P<0.05). Meanwhile, ATD exhibited an apoptosis-inducing effect on WM35 cells in a drug-and-dose dependent manner(P<0.05). Expression of Bax increased while Bcl-2 decreased with the increase of the concentration of ATD(P<0.05). Conclusion ATD exhibites marked effect of proliferation inhibition and apoptosis-inducing on WM35 cells. ATD activates the apoptosis pathway of key enzyme. The apoptosis associated protein Bax is up-regulated while that of Bcl-2 is down-regulated, which may be the mechanism of ATD in affecting the proliferation and differentiation of tumors.

Key words: WM35 cells, Apoptosis, Ar-turmerone derivatives

中图分类号: 

  • R739.5
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