您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 11-14.doi: 10.6040/j.issn.1671-7554.0.2014.097

• 基础医学 • 上一篇    下一篇

大麻素受体1与FAK在血吸虫肝纤维化小鼠肝组织中的表达

欧阳兵1, 彭忠田2, 王培3   

  1. 1. 南昌市第九医院感染一科, 江西 南昌 330002;
    2. 南华大学附属第一医院感染科, 湖南 衡阳 421001;
    3. 怀化市第一人民医院感染科, 湖南 怀化 418000
  • 收稿日期:2014-02-26 修回日期:2014-09-04 出版日期:2014-09-10 发布日期:2014-09-10

Expression and significance of cannabinoid receptor 1 and FAK in hepatic tissues of schistosomal hepatic fibrosis mice

OUYANG Bing1, PENG Zhongtian2, WANG Pei3   

  1. 1. Department of Infectious Diseases, Ninth Hospital of Nanchang, Nanchang 330002, Jiangxi, China;
    2. Department of Infectious Diseases, First Affiliated Hospital of University of South China, Hengyang 421001, Hu'nan, China;
    3. Department of Infectious Diseases, First People's Hospital of Huaihua City, Huaihua 418000, Hu'nan, China
  • Received:2014-02-26 Revised:2014-09-04 Online:2014-09-10 Published:2014-09-10
  • Contact: 彭忠田。E-mail:hncspzt@yahoo.com.cn E-mail:hncspzt@yahoo.com.cn

PDF (PC)

99

摘要： 目的 探讨大麻素受体1（CB1）与黏着斑激酶（FAK）在血吸虫肝纤维化小鼠肝组织中的表达。方法 将32只昆明小鼠分为正常组（n=10）和模型组（n=22），两组动物均普通喂养，8周后处死取肝组织。模型组小鼠采用腹壁贴附法建立血吸虫肝纤维化小鼠模型。根据纤维化程度将模型组分为Ⅰ级肝纤维化组（n=4）、Ⅱ级肝纤维化组（n=8）和Ⅲ级肝纤维化组（n=10）。HE染色观察病理变化，Masson染色观察肝纤维化程度，免疫组织化学法检测不同纤维化组CB1的表达，RT-PCR法检测各组CB1 mRNA及FAK mRNA的表达。结果 模型组可见明显虫卵结节及纤维化改变；不同纤维化组均可见CB1、CB1 mRNA及FAK mRNA表达，且随着肝脏纤维化程度的增加，CB1、CB1 mRNA及FAK mRNA的表达增加（P<0.05）。结论 CB1和FAK参与了血吸虫肝纤维化的发生与发展。

关键词: 大麻素受体1, 肝纤维化, 小鼠, 黏着斑激酶

Abstract: Objective To investigate the expression and significance of cannabinoid receptor 1 (CB1) and focal adhesion kinase (FAK) in hepatic tissues of schistosomal hepatic fibrosis mice. Methods A total of 32 Kunming mice were randomly divided into two groups: normal control group (n=10) and model group (n=22). The schistosome-induced liver fibrosis models were established by attaching cercaria to the skin on the ventral side of the mice and allowing infection to occur via direct penetration. All mice were raised normally, and at week 8 all mice were sacrificed to gain hepatic tissue samples. Mice in the model group were divided into 3 subgroups according to the severity of hepatic fibrosis. The pathological changes were determined by HE staining, the degrees of fibrosis were examined by Masson staining, the expression of CB1 was detected by immunohistochemical method, and the expressions of FAK mRNA and CB1 mRNA were tested by reverse transcription polymerase chain reaction (RT-PCR). Results In the model group, significant schistosome egg granuloma and hepatic fibrosis were observed, and CB1, CB1 mRNA and FAK mRNA were detected in all subgroups. Besides, with the aggravation of fibrosis, the expressions of CB1, CB1 mRNA and FAK mRNA increased. Conclusion CB1 and FAK play an important role in the genesis and progression of schistosomal hepatic fibrosis.

Key words: Focal adhesion kinase, Schistosomal hepatic fibrosis, Cannabinoid receptor-1, Mice

中图分类号: 

  • R381
[1] 陈峰,蔡卫民,陈智,等.吡喹酮与γ-干扰素对肝纤维化胶原代谢影响的实验研究[J].中华传染病杂志,2001,19(6):355-358. CHEN Feng, CAI Weimin, CHEN Zhi, et al. Effect of praziquntel and antifibrotic treatment on metabolism of collagen in liver fibrosis[J]. Chinese Journal of Infectious Diseases, 2001, 19(6):355-358.
[2] Starowicz K, Nigam S, Di Marzo V. Biochemistry andharm acology of endovan illoids[J]. Pharmacol Ther, 2007, 114(1): 13-33.
[3] Mechoulam R, Ben-Shabat S, Hanus L, et al. Identification of an endogenous 2-monoglyceride,present in canine gut, that binds to cannabinoid receptors[J]. Biochem Pharmacol, 1995, 50(1): 83-90.
[4] Munro S, Thomas K L, Abu-Shaar M. Molecular characterization of a peripheral receptor for cannabinoids[J]. Nature, 1993, 365(6441): 61-65.
[5] Mechoulam R, Fride E, Marxo V D. Endocannabinoids[J]. Eur J Phamacol, 1998, 359(1): 1-18.
[6] Berry E M, Mechoulam R. Tetrahydrocannabinol and endocannabinlids in feeding and appetite[J]. Pharmacol Ther, 2002, 95(11): 185-190.
[7] Calignano A, LaRana G, Giuffida A, et al. Control of pain initiation by endogenous cannabinoinds[J]. Nature, 1998, 394(15): 277-281.
[8] Caraceni P, Domen icali M, Giannone F, et al. The role of the endocannabinoid system in liver diseases[J]. Best Pract Res Clin Endocrinol Metab, 2009, 23(1): 65- 77.
[9] Mallat A, Lotersztajn S. Endocannabinoids and Liver Disease. I. Endocannabinoids and their receptors in the liver[J]. Am J Physiol Gastrointest Liver Physiol, 2008, 294(1): 9-12.
[10] Teixeira-Clerc F, Jalien B, Grenard P, et al. CB1 cannabinoid receptor antagonism: a new strategy for the treatment of liver fibrosis[J]. Nat Med, 2006, 12(2): 671-676.
[11] Schaller M D. Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions[J]. J Cell Sci, 2010, 123(7): 1007-1013.
[12] Brown S G, Thomas A, Dekker L V, et al. PKC-sensitizes Kir3. 1/3.2 channels to changes in membrane phospholipid levels after M3 receptor activation in HEK-293 cells[J]. Am J Physiol Cell Physiol, 2005, 2893(12): 543-556.
[13] 郝阳, 吴晓华, 郑浩, 等. 2007年全国血吸虫病疫情通报[J]. 中国血吸虫病防治杂志, 2008, 20(8): 401-405. HE Yang, WU Xiaohua, ZHENG Hao, et al. Schistosomiasis situation in People's Republic of China in 2007[J]. Chin J Schisto Control, 2008, 20(8): 401-405.
[14] 彭忠田, 李佳, 王培,等. 异甘草酸镁对纤维化大鼠肝脏TGF-β1及Smad蛋白表达的影响[J]. 现代生物医学进展, 2011, 11(2): 240-243. PENG Zhongtian, LI Jia, WANG Pei, et al.Effects of magnesium isoglycyrrhizinate on TGF-β/Smad signal expression in hepatic stellate cell in rats[J]. Progress in Modern Biomedicine, 2011, 11(2): 240-243.
[15] 王培,彭忠田,欧阳兵,等. 扶正化瘀胶囊对鼠血吸虫肝纤维化的作用及机制探讨[J]. 现代生物医学进展, 2012, 12(8): 1443-1448. WANG Pei, PENG Zhongtian, OUYANG Bing, et al. Therapeutical effects and mechanisms of fuzhenghuayu capsule in murine schistosomiasis liver fibrosis[J]. Progress in Modern Biomedicine, 2012, 12(8): 1443-1448.
[16] 依沙兰, 陈红, 邓永东, 等. 肿瘤坏死因子基因β多态性与乙肝后肝纤维化的相关性研究[J]. 第四军医大学学报, 2008, 29(1):36-39. Yi S L, Chen H, Deng Y D, et al. Correlation between tumor necrosis factor-β gene polymorphism and HBV-induced liver fibrosis[J]. Journal of the Fourth Military Medical University, 2008, 29(1):36-39.
[17] 高啸, 郭燕, 刘红艳, 等. 血吸虫性肝纤维化小鼠肝组织中内源性大麻素1受体的表达[J].中华肝脏病杂志, 2007, 15(5):389-390. Gao X, Guo Y, Liu H Y, et al. The expression of cannabinoid receptor 1 in fibrotic liver tissues of mice infested with Schistosoma mamsoni[J]. Chin J Hepatol, 2007,15(5):389-390.
[18] 杨莉, 赵召霞, 侯军良, 等. 大麻素受体1、FAK mRNA有小鼠肝纤维化形成过程中肝组织中的表达及相互关系[J].临床肝胆病杂志, 2011, 27(8):824-826. Yang L, Zhao Z X, Hou J L, et al. Hepatic expression of CB1 in rats with fibrosis and the relationship with FAK[J]. Journal of Clinical Hepatology, 2011, 27(8):824-826.
[19] 叶立红, 王羽巾奎, 刘云燕, 等. 大麻素受体1在慢性乙型肝炎肝纤维化发生和发展中的作用[J]. 中华肝脏病杂志, 2010, 18(9):707-708. Ye L H, Wang J K, Liu Y Y. The expression of cannabinoid receptor 1 in liver tissue of chronic hepatitis B patients involved in liver fibrosis[J]. Chin J Hepatol, 2010, 18(9):707-708.
[1] 杨晓倩 季静 刘娜 郭冬梅 崔癉. 三氯化铁及络合铁的抗银屑病作用研究[J]. 山东大学学报(医学版), 2209, 47(6): 114-117.
[2] 段淑红 刘凯 尹海燕 赵世斗 刘丰韬. 凝集素受体WGA、RCA和ECL在过量维甲酸致昆明小鼠腭裂发生中的作用[J]. 山东大学学报(医学版), 2209, 47(6): 47-.
[3] 索东阳,申飞,郭皓,刘力畅,杨惠敏,杨向东. Tim-3在药物性急性肾损伤动物模型中的表达及作用机制[J]. 山东大学学报 (医学版), 2020, 1(7): 1-6.
[4] 龙婷婷,谢明,周璐,朱俊德. Noggin蛋白对小鼠脑缺血再灌注损伤后学习和记忆能力与齿状回结构的影响[J]. 山东大学学报 (医学版), 2020, 1(7): 15-23.
[5] 焦凤萍,王玉,于爱莲,田兆菊,杨树林. 重组质粒pcDNA3.1(-)-P6-gBCTL-TBK-1的免疫效果评价[J]. 山东大学学报（医学版）, 2017, 55(3): 75-78.
[6] 李洪志,申永超,刘洁婷,赵孝金,初彦辉,袁晓环. 11β-HSD1抑制剂改善db/db小鼠胰岛素敏感性的实验研究[J]. 山东大学学报（医学版）, 2017, 55(10): 59-64.
[7] 展凤霞,丁娟,马蓓蕾,王谦. 雌二醇对Th1细胞极化与肝纤维化形成的影响[J]. 山东大学学报（医学版）, 2016, 54(8): 64-68.
[8] 郑倩倩,刘倜,谢克勤,林艺,李忠,王显军,毕振强,温红玲,宋艳艳,赵丽. 大蒜油在小鼠体内抗流感病毒作用[J]. 山东大学学报（医学版）, 2016, 54(7): 23-27.
[9] 赵栋燕,于岩波,左秀丽. 脑源性神经营养因子在小鼠肠黏膜屏障中的作用[J]. 山东大学学报（医学版）, 2016, 54(7): 1-5.
[10] 李懋禹,唐诗,杜怡峰. TRB3在APP/PS1转基因小鼠脑内的表达及其意义[J]. 山东大学学报（医学版）, 2016, 54(10): 1-5.
[11] 贾俊伟,周雪颖,李秋红,于凯,周国钰,周盛年. Annexin A7在匹罗卡品癫痫小鼠模型脑组织中的表达[J]. 山东大学学报（医学版）, 2016, 54(10): 6-10.
[12] 杨倩倩,孙高英,曹智新,尹海燕,王海波,李建峰. NLRX1在C57BL/6小鼠耳蜗毛细胞内的表达[J]. 山东大学学报（医学版）, 2016, 54(1): 11-16.
[13] 翟红运, 张登禄, 王光杰, 孔峰, 程广辉, 赵升田. 小鼠胚胎干细胞分泌因子对前列腺癌细胞作用的体外研究[J]. 山东大学学报（医学版）, 2015, 53(9): 8-12.
[14] 王镜涵, 李霄飞, 张志兵, 王海波, 李建峰. Prestin在Spag6基因敲除小鼠外毛细胞中的表达[J]. 山东大学学报（医学版）, 2015, 53(8): 16-22.
[15] 梁宇, 辛涛, 刘倩, 郑向荣, 杨依航, 庞琦. 茶氨酸对神经干细胞增殖和分化的影响[J]. 山东大学学报（医学版）, 2015, 53(4): 49-54.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发