不同剂量阿托伐他汀对扩张性心肌病大鼠心肌TGF-β1及心脏结构的影响

doi:10.6040/j.issn.1671-7554.0.2014.218

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 6-10.doi: 10.6040/j.issn.1671-7554.0.2014.218

不同剂量阿托伐他汀对扩张性心肌病大鼠心肌TGF-β1及心脏结构的影响

田艳¹, 侯善荣², 刘建立², 孙兴盛², 黄丽红¹

1. 潍坊医学院内科学教研室, 山东潍坊 261053;
2. 潍坊医学院附属医院心内科, 山东潍坊 261053

收稿日期:2014-04-14 修回日期:2014-09-04 出版日期:2014-09-10 发布日期:2014-09-10

Effects of different doses of atorvastatin on the content of TGF-β1 and cardiac structure of dilated cardiomyopathy rats

TIAN Yan¹, HOU Shanrong², LIU Jianli², SUN Xingsheng², HUANG Lihong¹

1. Department of Internal Medicine, Weifang Medical University, Weifang 261053, Shandong, China;
2. Department of Cardiology, Affiliated Hospital of Weifang Medical University, Weifang Medical University, Weifang 261053, Shandong, China

Received:2014-04-14 Revised:2014-09-04 Online:2014-09-10 Published:2014-09-10
Contact: 侯善荣。E-mail:hhs3191@126.com E-mail:hhs3191@126.com

摘要/Abstract

摘要： 目的研究不同剂量阿托伐他汀对阿霉素所致扩张性心肌病大鼠心肌转化生长因子β1（TGF-β1）含量及心脏结构的影响。方法 SD雄性成年大鼠60只随机分为正常对照组、扩心模型组、低剂量给药组、高剂量给药组，每组15只。扩心模型组和低、高剂量给药组给予阿霉素[2mg/（kg·w）]连续腹腔注射6周建造扩张性心肌病模型，低、高剂量给药组给予阿托伐他汀[5mg/（kg·d），20 mg/（kg·d）]灌胃，正常对照组给予等剂量的生理盐水腹腔注射及灌胃治疗。6周后行心脏彩超检查，并取组织行病理切片检查，ELISA法检测大鼠血清TGF-β1含量，免疫组化法检测大鼠左心室心肌TGF-β1的表达情况。结果低、高剂量给药组心肌病理学显示心肌细胞形态、纤维排列、炎症细胞浸润与扩心模型组相比有所改善；心脏彩超显示高剂量给药组左室扩张、心功能降低较扩心模型组明显改善；与扩心模型组相比，低、高剂量给药组血清TGF-β1含量显著降低（P<0.05），心肌TGF-β1蛋白表达明显降低，以高剂量给药组差异最为显著。结论阿托伐他汀能改善扩张心肌病心脏结构改变，抑制心肌纤维化的形成过程，与低剂量相比，高剂量阿托伐他汀作用更显著。

关键词: 转化生长因子-β1, 扩张性心肌病, 阿托伐他汀, 心脏结构

Abstract: Objective To investigate effects of different doses of atorvastatin on the content of TGF-β1 and cardiac structure of dilated cardiomyopathy (DCM) rats induced by adriamycin. Methods A total of 60 senior male SD rats were randomized into control group, model group, low-dose atorvastatin group, and high-dose atorvastatin group, with 15 animals in each group. DCM models were induced by intraperitoneal injection of adriamycin[2mg/(kg·w)] for 6 weeks. The low- and high-dose atorvastatin groups received different dosages of atorvastatin injection[5 mg/(kg·d), 20 mg/(kg·d)]. The control group were given the same dose of normal saline and and lavage. After 6 weeks, echocardiography was performed to observe the pathological changes in the myocardium. The level of TGF-β1 in serum was detected with ELISA, and TGF-β1 protein expression was measured with immunhistochemistry. Results Compared with the control group, the myocardial cell morphology, fiber arrangement, inflammatory cell infiltration of the low- and high-dose atorvastatin groups were improved. The echocardiograph showed that the enlargement of left ventricular and heart function were improved. Serum TGF-β1 levels were lower in low- and high-dose atorvastatin groups (P<0.05). The expression of TGF-β1 protein in myocardium was lower in atorvastatin groups, and the decrease in the high-dose atorvastatin group was more significant. Conclusion Atorvastatin can improve cardiac structure of DCM and restrain myocardial fibrosis. Compared with small dose of atorvastatin, high dose is more effective.

Key words: Cardiac structure, Dilated cardiomyopathy, Atorvastatin, Transforming growth factor-β1

中图分类号:

R574

田艳, 侯善荣, 刘建立, 孙兴盛, 黄丽红. 不同剂量阿托伐他汀对扩张性心肌病大鼠心肌TGF-β1及心脏结构的影响[J]. 山东大学学报（医学版）, 2014, 52(9): 6-10.

TIAN Yan, HOU Shanrong, LIU Jianli, SUN Xingsheng, HUANG Lihong. Effects of different doses of atorvastatin on the content of TGF-β1 and cardiac structure of dilated cardiomyopathy rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 6-10.

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不同剂量阿托伐他汀对扩张性心肌病大鼠心肌TGF-β1及心脏结构的影响

Effects of different doses of atorvastatin on the content of TGF-β1 and cardiac structure of dilated cardiomyopathy rats

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