Table of Content

  

24 December 2006
Volume 44 Issue 12

Articles


Articles

Adenosine triphosphate promotes the apoptosis in cultured cerebral cortex neurons of embryonic rats

BI Ai-ling,GAO Jian-xin,LIU Ke-jing,ZHOU Yu-qin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1194-1196. 

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To explore the role of adenosine triphosphate (ATP) of different concentrations in cultured cerebral cortex neurons of embryonic rats. Methods: The inverted microscope was used to observe the nature of dead cells and to count the number of the cells. Neuronspecific enolase(NSE) and cleaved caspase3 immunohistochemistry technique was used to determine the number of NSEpositive neurons and to observe the expression of caspase3 protein. Results: Both the number of cultured cells and the NSE positive neurons decreased obviously with ATP (10?mmol/L and 100?mmol/L) treatment, but the number of the cleaved caspase3 positive neurons increased obviously. Conclusion: ATP has dosedependent cytotoxicity on cultured cerebral cortex neurons of embryonic rats and caspase3 signal is necessary for the cell death.

A new frame shift mutation, Arg856fs mutation, in cardiac myosin binding proteinC gene of Chinese populationswith hypertrophic cardiomyopathy

AN Gui-peng,LIU Xiao-man,AN Feng-shuang,ZHANG Yun,ZHANG Cheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1202-1205. 

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To explore the diseasecausing gene mutation in Chinese patients with hypertrophic cardiomyopathy (HCM), and to analyze the correlation between the genotype and the phenotype．Methods: The exons 1516, 18, 26, 28 and 34 of cardiac myosin binding proteinC gene in 76 patients with hypertrophic cardiomyopathy were amplified by PCR, and the PCR products were underwent SSCP analysis. If any abnormal band was detected on the gel, the suspicious PCR products were sequenced to detect the exact mutant point．One hundred normal persons were used as controls. Results: A frame shift mutation，Arg856fs mutation，in the exon 26 of the MYBPC3 gene was identified in a 52yearold male patient with HCM and failed to be detected in the 100 normal controls，which suggested the diseasecausing mutation． Conclusion: Cardiac myosin binding proteinC (MYBPC3) may be one of the main diseasecausing genes.

Effect of Zinc and ZincEDTA in inhibiting psoriasis

FU Jun,CUI Xi,LIU Zhen-jun,LIU Na,DING Bo,GUAN Yi-tao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1206-1208. 

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To study the effect of Zinc and ZincEDTA in inhibiting psoriasis. Methods: Kunming mice of mitotic division model of vaginal epitheliums were randomly divided into eight groups: negative control, positive control, low, middle and high dosage of ZnCl2, and low, middle and high dosage of ZnEDTA, and then they were mornitored following ten days consecutive intragastrically administration of saline, Methotrexate, ZnCl2 and ZnEDTA. And one hour after last administration, mice were administered colchicines by intraperitoneal injection and sacrificed by taking the cervical vertebrae off. The vaginal mitotic division was observed under light microscope. Results: ZnCl2 or ZnEDTA of different dosages could obviously inhibit the mitosis of vaginal epithelial cells (P＜0.05). No statistically significant was found between ZnCl2 or ZnEDTA and positive control group. Conclusion: ZnCl2 or ZnEDTA may inhibit the proliferation of epidermic cells in psoriasis animal model.

Relationship between coping style and psychological

GAO Wei,YANG Min,ZHANG Xiu-li,HAN Li-li,HUANG Ping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1213-1216. 

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To explore the relationship between coping style and psychological status in patients with cancer. Method: Sixty patients with cancer and thirty healthy controls were administered with Trait Coping Style Questionnaire (TCSQ), Symptom Checklist 90 (SCL90), selfrating depression scale(SDS) and selfrating anxiety scale(SAS). Results: The patient had higher scores on negative coping style than the control (P<0.01), higher scores on SCL90, distinctly in somatization, anxiety, depression, and psychodisposition than the control, and higher scores on SAS and SDS than the control(P<0.05??0.01).There were negative correlations between positive coping style and psychological status (P<0.05??0.005), but positive correlations between negative coping style and psychological status(P<0.05??0.005). Conclusion: The cancer patients have many psychologic problems, in which coping styles are the major factors.

Experimental study of Metoprolol on cardiac fibrosis in rabbits with acute myocardial infarction

WANG Qing,SUN Xue-lin,XING Qi-chong,YAN Su-hua,HU He-sheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1217-1220. 

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To investigate the precautionary and therapeutic action of Metoprolol on collagen remodeling after acute myocardial infarction(AMI) in New Zealand rabbits. Methods： MI was established by ligating the left ventricular branch of coronary artery in 24 New Zealand rabbits who were randomly divided into 2 groups, Metoprolol group and AMI group. And the other 8 rabbits were enrolled into sham operation group. After test, 8 rabbits survived in Metoprolol group, 10 in AMI group, and 7 in sham operation group. The serum level of IL6 was measured before operation, 1 day and 4 days after operation, and the level of hsCRP before operation, 1 day and 7 days after operation. The serum levels of PⅠCP and PⅢNP were measured before operation and at 1, 4，7, 14, 28, 56 days after operation in all New Zealand rabbits. All indexes were measured by ELISA. After the experimental animals were executed at 56 days, pieces of myocardium in farction zone and in uninfarction zone were excised, and the deposition content of collagen I and Ⅲ were measured by

Experimental study on expanded prefabricated flaps

XUE Wen-jun,WANG Hai-tao,WANG Ming-qing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1233-1237. 

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To investigate the survival of expanded prefabricated flaps and its mechanism, microcirculation and ultrastructural changes. Methods: A total of 36 New Zealand male rabbits were divided randomly into expanded prefabricated, expender lined and prefabricated groups. After the femoral vascular bundles were transplanted into the subcutaneous tissues of abdomen, expanders were implanted into the deeper dartos. For the expanded prefabricated group, the expansion was carried out at 7 days postoperatively; When the expander was fully expanded at 52 days, island flaps with the prefabricated vessels as the pedicles were formed. The flaps were measured by digital recording of survival area, laser Doppler flowmetry, immunhistochemistry, light microscope and electron microscope. Results:　When compared with other groups, the blood flow enhanced; the flap survival improved; VEGF, bFGF, VEGFR and bFGFR increased； blood capillary was stronger， and the ultrastructure significantly changed in the expanded prefabricated groups. Conclusion: Expanded prefabricated flap is good in survival and safe in transplantation; the growth of VEGF, bFGF, VEGFR and bFGFR accelerates the neoangiogenesis of expanded prefabricated flaps.

Epilepsy induced by cerebral arteriovenous malformations:angioarchitectural characteristics and embolization treatment

ZHANG Ji-qing,WANG Zhi-gang,DING Xuan,JI Yong,SHEN Xun,HAN Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1238-1240. 

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To explore the size, location and feeding artery of arteriovenous malformations(AVM) by DSA and the response of embolization of AVM for epilepsy. Methods: DSA was performed in 95 patients with cerebral AVM and endovascular treatment with NBCA or ONYX was performed in all; chisquare test was used to analyze the results. Results: There was a significant association between the seizure induced by cerebral AVM and the size and the location of AVM (all P<0.01), but no significant association between the number of feeding artery and the seizure (P>0.05); of the 43 patients (43/95,45%)　were found seizures, 20 had their symptoms disappeared after embolization, 11 had their symptoms relieved; the response rate was 72.1%. Conclusion: The bigger the AVM is and the more close the AVM is to the brain cortex, the more the patient are inclined to tempt seizure. The number of feeding artery has no relation with the epilepsy; and epilepsy has been greatly improved through endovascular embolization treatment for epileptogenic AVMs.

An animal experimental study of intraarticular injection of triamcinolone acetonide and sodium hyaluronate for the treatment of temporomandibular joint osteoarthrosis

LAI Qing-guo,SONG Dai-hui,XIE Kun,JIN Yanwu,YUAN Kuifeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1248-1252. 

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To investigate the efficacy of triamcinolone acetonide and sodium hyaluronate for treatment of temporomandibular joint (TMJ) osteoarthrosis of goats. Methods: The upper compartments of both joints of 9 goats were injected with collagenase to induce osteoarthrosis. The 9 goats were devided into 3 groups, 3 goats per group. The upper compartments of both TMJs in three goats in control group were injected with 1ml physiological saline and in the two treatment groups were injected with 1ml triamcinolone acetonide and sodium hyaluronate, respectively. The TMJs of goats were examined under light microscopy and scanning electron microscopy after being sacrificed, and were scored in a subjective manner following the standard criteria which was a modification of the method of Mankin, et al and Yoshimi, et al. Results: Under light microscopy, the treatment group with sodium hyaluronat showed a light osteoarthrotic changes and the treatment group with triamcinolone acetonide showed more severe osteoarthrotic changes when comparing with the control group. Result under scanning electron microscopy accorded with the result under microscopy. Conclusion: On the basis of 3month following up, intraarticular injection of triamcinolone acetonide will aggravate the osteoarthrotic changes while intraarticular

Association among genetic polymorphisms of HO-1, TNF-α and chronic obstructive pulmonary disease

JIANG Peng,LI Yu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1253-1257. 

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To investigate the relations among the genetic polymorphism of human Heme Oxygenase1 promoter region, tumor necrosis factorα and susceptibility of chronic obstructive pulmonary disease(COPD). Method: The genetypes of 45 severe, earlyonset COPD patients, 60 common COPD patients and 60 control subjects were tested with PCRRFLP to analyse the GT repeat in HO1 promoter region and the polymorphism of TNFα308. Results: There were no difference between severe, earlyonset COPD patients and common COPD patients about cigarette smoking (OR 3.64 vs 4.75)， while both groups had a significant difference when comparing with control subjects separately. The L type allele frequencies in HO1 promoter region and the A type allele frequencies in TNFα308 of severe, earlyonset COPD patients and common COPD patients all showed a statistical significance compared with the control subjects; The A type allele frequencies in TNFα308 of severe, earlyonset COPD patients had a higher odds ratio（11.88 vs 3.30）. Both the HO1L type allele frequencies in promoter region and the A type allele frequencies in TNFα308 of the two groups played an interactive role with cigarette smoking. Conclusions: The L type allele of HO1 promoter region and the A type allele of TNFα308 are associated with earlyonset COPD and increase the risk of developing earlyonset

Relationship between HBV infection and expression ofHLA molecules on host lymphocytes

DENG Xiao-mei,WANG Jin-feng,ZOU Xiong,YU Xiu-ping,YANG Xiao-jing,ZHENG Gui-xi,LIU Min

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1258-1260. 

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To examine the expression of HLA class Ⅰ and HLA class Ⅱ on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer and investigate the role of them on peripheral blood lymphocytes in host celluar immunity. Methods: The expression of HLA class Ⅰ and HLA class Ⅱ on peripheral blood lymphocytes were evaluated by flow cytometry (FCM) in 51 healthy persons, 58 patients with chronic hepatitis B, 58 patients with hepatic cirrhosis and 13 patients with hepatocellular cancer. Results: The mean fluoresecence intensity of HLA class Ⅰ on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer was 335.80, 309.78 and 114.04, respectively, which had a significant difference from that in the normal control(P<0.01). With the aggravation of disease, the mean fluoresecence intensity of HLA class Ⅰ on peripheral blood lymphocytes progressively decreaced in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer. The expressive percentage of HLA class Ⅱ in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer was 38.28%, 42.21% and 52.75%, respectively, and there was a significant difference in patients with hepatic cirrhosis and hepatocellular cancer from that in normal controls (P<0.01); but there was

Distribution of hepatitis B virus genotypes and subgenotypes in Shandong area

ZHOU Pei-jing,WANG Lei,GENG Da-ying,ZHUANG Hui,LI Ya-juan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1261-1264. 

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To investigate the distribution of hepatitis B virus (HBV) genotype and subgenotype in Shandong province. Method： HBV genotypes and subgenotypes were detected in 140 patients with HBV infection by using nested polymerase chain reaction based on genotype and subgenotype specific primers. Results: HBV genotype B, C and B/C were identified in 12, 109 and 8 patients, whereas genotype A, D, E and F were not found. All of the 12 HBV genotype B patients were detected as subgenotype Ba. Of the 109 genotype C patients, 74.3% (81/109) was subgenotype C2 and 25.7% (28/109) was neither C1 nor C2. Of the 8 genotype B/C patients, 5 were subgenotype Ba+C2 and 3 were Ba + neither C1 nor C2. Conclusions： Genotype B, C and B/C have been found in Shandong province, and subgenotype Ba inhabits the genotype B and subgenotype C2 predominates genotype C.

Periprosthetic bone mineral density after total knee arthroplasty

SUN Xiu-jiang,WANG Shao-jin,LIU Wen-guang,WANG Xing-shan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1265-1267. 

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To evaluate the changes of periprosthetic bone mineral density(BMD) after total knee arthroplasty. Methods: Sixteen patients treated by cemented total knee arthroplasty were included in this study. The periprosthetic bone mineral density was measured by dualenergy Xray absorptiometry 1 week, 3 months, 6 months and 12 months postoperatively. The periprosthetic bone of distal femur and proximal tibia were divided into four and three regions of interest separately, and the mean BMD was measured. Results: The mean BMD of all seven regions decreased within 12 months after operation (P<0.05) and of distal femur and proximal tibia decreased by 15.73% and 15.16%, respectively. Bone loss was the most rapid during initial 3 months. Conclusions: The periprosthetic bone mineral density significantly decreases after total knee arthroplasty, which may be measured precisely by dualenergy Xray absorptiometry.

Effects of thoracic epidural anesthesia on the intramucosal pH value

WANG Huan-liang,SUN Bao-zhu,WANG Chun-ling,ZHANG Li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1281-1283. 

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To investigate the effect of thoracic epidural anesthesia (TEA) on the intramucosal pH (pHi). Methods: Thirty ASAⅠ～Ⅱ patients who were going to undergo colectomy randomly were divided into TEA and control groups. 0.5% bupivacanine of 8?ml or isotonic sodium chloride solution was injected into the epidural space. Gastric mucosal tonometry was placed into the stomach to measure the intramucosal CO2 (PiCO2) and the pHi was calculated before and 60, 120, 180, 240?minutes after epidural injucation. Meanwhile, the concentration of corticoserone and blood glucose were titrated. Results: pHi and PiCO2 had a statistical significance between the groups of 180 and 240 minutes, and the maximal pHi significantly decreased (P<0.01) in the control group (0.16±0.04) compared with that in the TEA group (0.07±0.05). There were 10 patients in the control group and 2 in the TEA group whose pHi<7.32 at the end of the study (P<0.01). There were significant differences between groups in the concentration of corticoserone and blood glucose (P<0.05～0.01). A negative correlation was established between the pHi and the concentration of corticoserone（r=-0.58, P<0.01）. Conclusion: TEA can prevent the decrease of pHi induced by surgical stress and increase the flow of visceral perfusion.

Expression of LATS1 and LATS2 in primary glioma by SYBRGreen quantitative realtime PCR

JIANG Zheng,HU Jin,LI Xingang,LU Daru,JIANG Yuquan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(12):  1289-1293. 

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To detect the expression of LATS1 and LATS2 mRNA in human primary glioma by SYBR Green quantitative realtime PCR, and to analyse the relationship between the expression and the clinical data. Methods: The method of SYBR Green quantitative realtime PCR was established. The expression of tumor suppressor gene LATS1 and LATS2 in 30 primary glioma tissues (WHO：GradeⅡ10 cases, Grade Ⅲ10 cases and Grade Ⅳ 10 cases) and 10 normal brain tissues were quantitated by the method of standard curve. The expression differences of LATS1 and LATS2 in different grade glioma compared with the normal brain tissues were studied and the relationship of the expression and the clinical data was also analyzed by a statistical method. Results: The expressions of LATS1 and LATS2were all significantly downregulated in different grade glioma compared with normal brain tissues (P＜0.05). But there were no significant differences in the expression of LATS1 and LATS2 among the different grade glioma (P＞0.05). And there were also no significant differences in different gender and age. Conclusions: The method to detect the expression of LATS1 and LATS2 by SYBR Green quantitative realtime PCR is established successfully. The tumor suppressor genes LATS1 and LATS2 are all downregulated in human glioma compared with those in the normal brain tissues.