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Table of Content

      
    24 November 2006
    Volume 44 Issue 11
    Articles
    Role of cytokeratin in determining the lymph node micrometastasisin gastric carcinoma
    XIA Qing,CHEN Xue-liang,WANG Xiu-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1081-1084. 
    Abstract ( 1250 )   Save
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    To investigate the role of cytokeratin in determining the lymph node micrometastasis in nodenegative gastric carcinoma with anticytokeratin antibody. Methods:Two hundred and fortytwo dissected lymph nodes from 50 patients with nodenegative gastric carcinomas were examined with hematoxylin and eosin (HE), antihuman CEA (carcinoembryonic antigen) antibody, anticytokeratin 19 (CK19) antibody and anticytokeratin 20 (CK20) antibody. Of them, 28 patients were followed up for 48 to 90 months. Results: Micrometastases were identified in 11 of 50 patients (22%) and in 18 of 242 lymph nodes (7.44%) by immunohistochemical staining. Positive reactions were found in 10 lymph nodes (4.13%) stained with CEA, and in 17 (7.02%) or 16 (6.61%) stained with CK19 or CK20. No lymph node stained by HE showed positive result. A higher incidence of micrometastasis was found in the patients with recurrent disease (42.86%, 3/7) compared with result from recurrencefree patients (9.52%, 2/21). Survival analysis demonstrated that the diseasefree survival time in micrometastasis positive patients was shorter than that of the negative ones. But there was no significant difference of the cumulative survival rate. Conclusions:CK is a more sensitive marker than CEA for detection of metastases, and the specificity of CK20 is better than CK19 in this assay. Micrometastases might correlate with worse prognosis for patients with histologic node negative gastric cancer.
    Umbilical cord blood stem cells: culture and
    CHEN Zeng-hai,MA Wan-li,GAO Chun-zheng,CHEN Peng,REN Peng,JIANG Xi-feng,LI Gui-xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1085-1089. 
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    To study an experimental technology for repairing injured spine by transplantation of cord blood stem cells. Methods: (Human mesenchymal stem cells, hMSCs) were separated from cord blood by density gradient centrifuging and cultured in DMEM with 20% FBS. Amplified and taken the 5th generation to have an innoculation in the culture plate, they were put into the culture medium added inducer and cultivated at 37?℃, 5% CO2 in satured humidity. The neuron symbol NSE, NF and the astrocyte symbol GFAP were detected by immunohistochemical method, then under the microscope, 10 unwraped eyeshot was counted randomly and calculated the positive percentage of NSE, NF and GFAP denoting as ±s. Result: The hMSCs seemed like neurons and changed into taper cells with many long tuber and cytoplast shrinking to the nucleus which became denser and more refracting. Twentyfour hours after hMSCs were incubated in beforehand 3.63)% of NSE, (33.46±3.21)% of NF and (15.12±1.65)% of GFAP were found. Conclusion: Cord blood stem cells have a relatively strong ability of proliferation and a potent of differentiating into neuronlike cells at a certain condition.
    Proliferation of fibroblastlike synovial cells in patients withrheumatoid arthritis in vitro
    SHU Qiang,LI Xingfu,LIU Hua-xiang,LI Dong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1095-1099. 
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    To investigate the proliferation of fibroblastlike synovial(FLS) cells in rheumatoid arthritis (RA) patients in vitro and the immunosuppressive effect of thalidomide, total glucosides of paeony (TGP) and T614 on them. Methods: Synovial specimens were obtained from 6 patients with RA by arthroscopy and blindly needle biopsy, or by pumping synovial fluid on knees. FLS cells were identified in vitro by histopathological dyeing, immunohistochemical analysis and fluorescenceactivated cell sorting(FCS)method for cell surface molecules respectively. The synoviocyte proliferation was measured by MTT colorimetric assay and the survival fraction(SF)of FLS was evaluated in the presence or absence of thalidomide, TGP and T614. The expression of cfos mRNA was examined by semiquantitative RTPCR in FLS cells of RA patients. Results: The identified synovial cells in patients with RA were FLS(Vimentin and Fibronectin expression positive)either cultured with agents or not. The SF of all FLS interfered by thalidomide, TGP orT614 was lower than vacant contrast without agents in RA shown by MTT assay(P<0.05). Thalidomide operated the strongest, whereas T614 the weakest in the three agents(P<0.05). Thalidomide at physiological doses and TGP in high concentration demonstrated marked dosedependent inhibitory curses on FLS proliferation efficiency(P<0.05). It was detected by semiquantitative RTPCR that cfos mRNA of RAFLS expressed highly and decreased obviously when incubation with thalidomide, TGP or T614. In all the three agents, thalidomide downregulated cfos expression markedly(P<0.05). Conclusion: There are no distinct activated unlimited viability in RAFLS without being stimulated by proinflammatory cytokine in vitro. The results have confirmed our suggestion that thalidomide, TGP and T614 possess the immunosuppressive effect to synovitis by modulating the cell proliferation and cfos mRANA gene transcription directly in RAFLS.
    A simple and sensitive method for screening εPL producing strains from soils
    ZHANG Chao,ZHANG Dong-rong,HE Wei,DUAN Zuo-ying,MAO Zhong-gui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1104-1107. 
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    To develop a simple and sensitive method for screening εPL producing strains from soils. Methods: Because dye reacted with the secreted basic polymers by electrostatic interaction and formed special zoon, a basic dye, methylene blue, incorporated in the agar plate was used to detect εPL producers, and then Dragendorff reagent was used to detect alkaloid producers. Result: A over producing strain Z18 was obtained. Conclusion: Z18 is a member of Streptomyces albulus.
    Growth inhibition of BIBR1532 to cancer cells with
    ZHANG Jing,LIU Zhao-xu,YANG Jin-ling,CHEN Xin-xia,LOU Feng-lan,XU Da-wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1113-1116. 
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    To study the inhibition effect of BIBR1532 on telomerase activity and cell growth of cancer cells with p53 and pRb inactivation. Methods: The cancer cells with both p53 and pRb inactivation and the telomeraseimmortalized human BJ fibroblasts with normal p53 and pRb were treated with BIBR1532, and the telomerase activity, telomere length and cell growth status were detected. Results: The cells treated with BIBR1532 exhibited diminished telomerase activity, shortened telomeres and eventual growth arrest independent of p53 and pRb status. Conclusion: Telomere shorteningmediated cell growth arrest caused by telomerase inhibition in cancer cells does not require functional p53 and pRb.
    Association between polymorphisms of killer cell immunogloblinlikereceptor gene and ankylosing spondylitis
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1117-1120. 
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    To investigate whether the polymorphisms of killer cell immunoglublinlike receptor(KIR) gene has relation with the ankylosing spondylitis (AS). Methods: Polymerase chain reactionsequencespecific primers (PCRSSP) method was employed for genotyping KIRs from genomic DNA of 86 AS patients and 412 healthy donors. Results: Gene frequencies of KIR3DS1(P=0.044), KIR2DL2(P=0) and KIR2DL5(P=0.01)were statistically significant higher in the patient group than in the control group. Moreover, the percentage of patients carrying two or more activating KIRs was higher than that of controls. The frequencies of the other KIR loci subjected to assay in this work showed no significant difference in patients and controls. Conclusion: The data from this study suggest that the imbalance of KIR genes may have an association with a genetic predisposition to ankylosing spondylitis.
    Effects of NacetylLcysteine on the fetal ovarian tissues cultured in vitro
    WANG Fei,LI Hui,CUI Min,YANG Zhong-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1128-1131. 
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    To investigate the effects of antioxidant NacetylLcysteine(NAC) on the fetal ovarian tissues cultured in vitro. Methods: Fetal ovaries of 20 to 28 weeks were cultured for 0 to 9 days in 4 NAC groups: 25?mmol/L, 50?mmol/L, 100?mmol/L and 0?mmol/L. The concentrations of estrogen in the culture medium were measured by radioimmunoassay and the morphologic changes of the cultured ovarian tissues were observed. Results: The proportion of matured follicles of different stages in the 3 experimental groups were significantly different compared with the control group (P<0.05) and the estrogen output in both control and experimental groups were significantly increased with the time prolonged(P<0.05), however, the differences among the experimental groups were not significant (P>0.05); the estrogen output of the fetal ovarian tissues in the 3 experimental groups were significantly increased than that in the control group (P<0.05), however, the differences among the experimental groups were not significant (P>0.05). Conclusion: NacetylLcysteine can increase the estrogen output and the proportion of mature follicles in the fetal ovarian tissues cultured in vitro.
    Personality traits and parental rearing practices in adolescent with adjustment disorders
    MAO Xue-qin,HAN Jie,LIU Min-ying,WANG Meng-xin,PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1132-1134. 
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    To explore the personality and parental rearing practices in adolescents with adjustment disorders. Methods: The personality and parental rearing practices in adolescents with adjustment disorders and the control were assessed with questionaires of MMPI and EMBU. Results: Contrasting with the control, the case group had significantly higher scores of factor Hy, Pd and Pt in MMPI, and lower scores of father′s factor 1 (F1), higher scores of father′s factor 2, 3, 4, 5 (F2, F3, F4, F5, respectively) and mother′s factor 2, 3, 4, (F2, F3, F4, respectively) in EMBU. There were statistical correlations between factors of MMPI and EMBU. Conclusion: Adolescents with adjustment disorders have unhealthy personality traits. The parental rearing practices of the case group are harmful to the development of personality.
    Expression of Cyclooxygenase2 in human transitional cell bladder carcinoma
    MA Tian-ia,ZHOU Ya-in,ZHANG Huai-iang,ZHOU Chun-Wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1139-1141. 
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    To investigate the expression of Cyclooxygenase2(COX2) in human transitional cell carcinoma of bladder(BTCC). Methods: The expression of COX2 mRNA was examined by reverse transcriptasepolymerase chain reaction (RTPCR). The protein expression of COX2 was examined by immunohistochemical staining. Results: Upon RTPCR, 13 frozen specimens from bladder cancer were shown to express COX2 mRNA, while none of 7 specimens from normal bladder mucosa expressed COX2 mRNA. The difference was significant. The immunohistochemical results was analogous to the RTPCR results. COX2 protein was mainly localized at cytoplasm of malignant cells (positivity rate was 55.74%), but was not expressed in normal bladder mucosa. Conclusion: The expression of COX2 mRNA and protein is enhanced in human BTCC and is associated with the malignant degree of the tumor, indicating that COX2 may play a key role in the development and progression of BTCC.
    The contour area multifractal model on the spatial distribution of disease and its application
    TANG Fang,XUE Fu-zhong,WANG Jie-zhen,KANG Dian-min,WANG Zhi-qiang,HU Ping,ZHANG Yu-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1154-1158. 
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    To explore the application of contour area multifractal model (CAMM) in identifying the interval cutoff vales of mapping interpolated disease. Methods: Combine multifractal with statistics in the framework of AreGIS9.0 to set up the CAMM. Results: The CAMM was set up, and its stepbystep procedure was presented. To validate its applicability in mapping disease, the structures and boundaries of spatial heterogeneity of hemorrhagic fever with renal syndrome (HFRS) were identified by using CAMM. Conclusion: The structures and boundaries of spatial heterogeneity of disease identified by CAMM can display the spatial characteristics of disease accurately.
    Knowledge, attitudes and practices of Jinan ruralurban fringe population towards chronic metabolic disease: a crosssectional study
    CHANG Cai-yun,WANG Shu-mei,FENG Yue-qiu,GAO Li-jie,FANG Xue-qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1167-1170. 
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    To learn the status on knowledge, attitudes, practices of people with chronic metabolic disease in Jinan urbanrural binding region and provide evidence for chronic metabolic disease preventive policy and measure in these areas. Methods: In the crossover study, 394 persons in two communities of Jinan urbanrural binding region were interviewed face to face, and fasting plasma glucose and blood fat were tested uniformly. Results: About 37.0% of the subjects knew chronic metabolic disease, and 20.8% of them knew little about the disease. Of the 125 individuals previously diagnosed, 104 were taking medicine; however, only 57 participants were taking medicine according to how it was prescribed by their physicians. The main reason for not taking the medicine as recommended by the physicians was a failure to realize the imperilment of the chronic metabolic disease, accounting for 47.2%. Multivariable analysis showed that degree of education was a significant factor in the knowledge level of chronic metabolic disease. Conclusion: The residents′ knowledge about chronic metabolic disease and treatment status are unsatisfying, so it is imperative to promote chronic metabolic disease knowledge awareness through raising residents′ cultural level and to strengthen health education.
    Expressions and significances of EGFR and Ki67 in nasal inverted papilloma
    LI Jing,WANG Bao-hua,
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1171-1174. 
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    To detect the expressions of epidermal growth factor receptor (EGFR) and Ki67 in inverted papilloma of nasal cavities and paranasal sinuses and investigate their clinicopathological significances in occurrence and aggravation of this disease. Methods: Immunohistochemical technique (SP staining method) was used to detect the expressions of EGFR and Ki67 in 17 cases of inverted papilloma(IP) arising from nasal cavity and paranasal sinuses and 7 cases of squamous cell carcinoma aggravating from inverted papilloma (IP+SCC) and 12 cases of nasal polyp tissues(NP). Results: The labelling index (LI) of Ki67 positive cells was significantly higher in IP+SCC than in IP(F=12.87, P=0.001?6) and NP(F=17.50, P=0.000?6), and a significant difference was also found between IP and NP(F=6.45, P=0.017?2). EGFR expression rate was 11.76%(2/17), 57.14%(4/7) and 8.33%(1/12) in IP, IP+SCC and NP respectively, and significant differences were found between IP+SCC and IP(χ2=6.708, P=0.035), between IP+SCC and NP(χ2=7.937, P=0.018?9), respectively, but no significant difference was found between NP and IP(χ2=0.000?2, P=0.989?6). Conclusions: Immunohistochemical features of Ki67 indicate that increased epithelial cell proliferation seems to be involved in the development and aggravation of nasal cavity and sinuses inverted papilloma. EGFR may play an important role in arising squamous cell carcinoma from inverted papilloma of nasal cavity and paranasal sinuses, but has no relation with the occurrence of nasal inverted papilloma.
    Overexpressions of PTTG and VEGFC in nonsmall cell lung cancer and their clinical significances
    GUO Xu-feng,HU Guo-qiang,YIN Qiu-wei,ZHAO Jian,CHEN Wei-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1175-1179. 
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    To investigate the lymphatic microvessel density and expressions of pituitary tumor transforming gene and vascular endothelial growth factor C in nonsmall cell lung cancer(NSCLC),and explore their correlations to clinicopathologic features of NSCLC. Methods:Realtime fluorescence quantitative PCR and immunohistochemistry method were used to detect the expressions of PTTG, VEGFC and LMVD in NSCLC. We also investigated the effects of PTTG and VEGFC on the prognosis of patients with NSCLC by drawing KaplanMeier curves. Results: There were higher expressions of PTTG,VEGFC and LMVD in NSCLC tissues than in adjacent tissues and benign lesions(P<0.05). There were no significant differences between the expressions of PTTG, VEGFC and age, sex, smoking, and size, histological type and differentiation of tumor(P>0.05), but there were statistical relationships between their expressions and TNM stage, lymph node metastasis and prognosis(P<0.05). The KaplanMeier curves showed that patients with low or no expressions of PTTG or VEGFC survivedpositively correlated with VEGFC expression and LMVD. Conclusion: Tumor lymph angiogenesis is enhanced by the overexpression of PTTG and VEGFC; PTTG and VEGFC can be used as two useful markers to judge the biological behavior and the prognosis of lung cancer; VEGFC can be used as a new target of antilymphatic therapy for lung cancer.
    nm23 and CD44v6 protein expression and microvessel density relate to the prognosis of rectal carcinoma
    LI Ning,WANG Xu-zhou,XU Fu-yu,WANG Yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(11):  1180-1183. 
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    To investigate the expression of nm23, CD44v6 and the microvessel density (MVD) in rectal carcinoma and analyze their relationship with prognosis. Methods: The expressions of nm23 and CD44v6 and the MVD were examined using immunohistochemical technique in 70 cases of rectal carcinoma, and the prognosis was analyzed. Results: The expression of nm23 had correlation with the depth of infiltration, the UICC staging and the lymph node metastasis. Both the expression of CD44v6 and the MVD had correlation with the histological type, the depth of infiltration, the UICC staging and the lymph node metastasis. KaplanMeier survival analysis revealed that the survival rate was correlated with the UICC staging, the lymph node metastasis and the expressions of nm23 and CD44v6 and the MVD. However, Cox proportional hazards analysis showed that only UICC staging and the expression of nm23 retained independently in prospecting prognosis. Conclusion: The UICC staging and the expression of nm23 are considered as the independent parameters in predicting the prognosis of rectal carcinoma.