JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

Progesterone combined with octreotide inhibits the growth of human non-small cell lung cancer A549 cells

SUN Mei-li, WANG Xiu-wen, WEI Jun-min   

  1. Center for Tumor Treatment and Prevention, Qilu Hospital of Shandong University, Jinan250012, Shandong, China
  • Received:2006-09-04 Revised:1900-01-01 Online:2007-01-24 Published:2007-01-24
  • Contact: WANG Xiu-wen

PDF (PC)

697

Abstract: Objective: To investigate the regulation of somatostatin recepter (SSTR) subtype mRNAs expression of non-small cell lung cancer cell line A549 by progesterone and the antiproliferation of progesterone combined with octreotide on A549 cells. Methods: The progesterone receptor was detected by the immunohistochemical method, and SSTR subtype mRNAs were determined by the reverse transcription polymerase chain reaction in cells pretreated or non-pretreated by progesterone. The cell viability was evaluated by MTT assay. Results: Progesterone receptor and SSTR1, SSTR2, SSTR4 and SSTR5 were revealed in A549 cells, but SSTR3 was not. Following 24-hour treatment by 10nmol/L progesterone, SSTR3 was detected in A549 cells. Up to 48 hours, SSTR3 was still up-regulated(P＝0.012). Progesterone combined with octreotide was superior to progesterone or octreotide alone in inhibiting the growth of A549 cells(P<0.05). Conclusion: Progesterone up-regulates the expression of mRNA for SSTR subtypes in A549 cells. Progesterone enhances the ability of octreotide to inhibit the growth of A549 cells.

Key words: Carcinoma, non-small cell lung, Somatostatin receptor, Progesterone

CLC Number: 

  • R737.33
[1] YU De-Xin, CAEI Ti-Gong, MA Xiang-Xing, ZHANG Xiao-Ming, LI Chuan-Fu. Angiogenesis and maturation of hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 51-54.
[2] WANG Shao-Qing, LIU Yi-Hui, FU Ting-Ting, CHENG Jin-Yong, LIU Qiang. Classification of 31P MRS data for hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 42-46.
[3] LV Yan-Feng, DING Ke, WANG Jian-Xin, YU Hua-Long, BEI Shao-Sheng. Canceration of chronic anal fistula [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 55-57.
[4] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[5] LI Yinglin, SONG Daoqing, XU Zhonghua. Identification of FKBP11 expression in clear cell renal cell carcinoma using bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 45-51.
[6] MENG Zhaotun, LI Qin, SUN Hui, WU Xinfang, SUN Wenkai, GAO Wei. Correlation between interleukin-18 single nucleotide polymorphism and susceptibility of laryngeal squamous cell carcinoma: a report of 73 cases [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 58-63.
[7] LUO Xin, HE Bing, NIE Qingsheng, HOU Zhenbo, DONG Jun, LI Yuhua, ZENG Xiangqin, LIU Wei, KONG Demin, CAO Jinfeng. Comparison of the value of mono-exponential mode and diffusion kurtosis imaging mode in grading clear cell renal cell carcinoma using magnetic resonance diffusion-weighted imaging [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 89-95.
[8] ZHAO Zuohui, LI Cuiling, WANG Daoguang, WANG Fengqin, QU Hongyi, DING Sentai, GONG Jing, LÜ Jiaju, YANG Jinghua. Effect of MnSOD acetylation on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 31-35.
[9] LI Xingkai, LIU Zhanye, JIANG Yunfeng, LI Jun. Clinicopathological factors and prognosis between primary central and peripheral lung squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 73-78.
[10] WANG Ningning, QU Linli, CAO Fangli, LIANG Lei. Application of computed tomography sagittal reconstruction for microwave ablation of hepatic carcinoma under the diaphragm [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 71-75.
[11] TANG Xi, HU Ya, XU Yanhua, WANG Chunlin, QIU Ping, WANG Xianghui. MiR- 498 inhibits A549 cells EMT by targeting FOXM1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(4): 39-43.
[12] SUN Qijing, CHEN Fangfang, LI Chunxiao, ZHANG Caiqing. Clinical value of PNI and HGB in evaluating the prognosis of the middle to late stage non-small cell lung cancer patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(4): 55-59.
[13] ZHANG Zhihui, WANG Lili, GAO Hua, ZHANG Jian, LI Juan, LI Yuan, WU Chunxiao, LU Zhiming. Role of hypoxia-inducible factor-1α in the regulation of programmed death ligand 1 expression in lung adenocarcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(4): 65-70.
[14] WANG Yongqiang, ZHANG Weiquan, SUN Qifeng, DONG Xiaopeng, PENG Chuanliang, ZHANG Yuan, ZHAO Xiaogang. Expression and significance of CK14 in non-small cell lung cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 83-87.
[15] SHI Peikun, ZENG Beini, WU Weifang, HU Xiaoyan, MA Tianjia, ZHOU Yabin. Expression and role of Keap1 in renal cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 94-99.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd