Abstract: To investigate the relation between the single nucleotide polymorphisms(SNPs) of tumor necrosis factorα(TNFα) or interleukin6(IL6) promoter genes and susceptibility to the patients with gastric adenocarcinoma or the patients with gastric adenocarcinoma with helicobacter pylori(HP) infection. Methods：The SNPs of the TNFα(238G/A and 308G/A) and IL6(597G/A,174G/C and 572G/C) were determined by gene chip in 65 patients with gastric adenocarcinoma and 71 healthy controls. The sera concentrations of IgG, IgM and IgA of HP antibodies were measured by ELISA. Results：H. pylori infection was detected in 69.2% of 65 patients and 46.5% of 71 controls (P=0.007, odds ratio ［OR］=2.53，95% confidence interval ［95%CI］=1.28-5.24). Frequencies of TNFα238GA genotype and A allele in patients with gastric adenocarcinoma were significantly higher than those in healthy controls(P=0.024， OR=2.44，95%CI=1.11-5.37 and P=0.039，OR=2.13，95%CI=1.03-4.41, respectively). Frequencies of TNFα238GA genotype and A allele in patients with gastric adenocarcinoma with HP infection were significantly higher than those in HPnegtive patients with gastric adenocarcinoma(P<0.05，OR=4.53,　95%CI=1.16-17.68 and P<0.05，OR=3.52,　95%CI=0.9812.64,respectively). The proportion of individuals carrying the IL6572CC genotype was significantly lower in patients with gastric adenocarcinoma than in the controls(P=0.014，　OR=0.17， 95%CI=0.04-0.81). No association was found between any of the other polymorphisms and patients with gastric adenocarcinoma or patients with gastric adenocarcinoma with HP infection. Conclusion：There are significant correlation between TNFα238GA genotype and A allele and gastric adenocarcinoma or gastric adenocarcinoma with HP infection. IL6572CC genotype is a protective function against cancers.

Key words: Stomach neoplasms, Tumor necrosis factorα, Interleukin6, Polymorphism, single nucleotide, Helicobacter pylori, Olignucleotide array sequence analysis