Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (12): 26-32.doi: 10.6040/j.issn.1671-7554.0.2018.786

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Correlation between peripheral Syntaxin 8 expression and chronic inflammation and glucose-lipid metabolism in type 2 diabetes mellitus patients

CHEN Pan, JIANG Chunxia, LEI Yi   

  1. Department of Endocrinology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan, China
  • Published:2022-09-27

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Abstract: Objective To investigate Syntaxin 8(STX8)expression in patients with type 2 diabetes mellitus(T2DM)and the potential correlations between STX8 and chronic inflammation and glucose/lipid metabolism. Methods A total of 144 T2DM paitents(case group)and 100 health subjects(control group)were enrolled. The difference of clinical parameters such as inflammatory indices and glucose/lipid metabolism were compared between two groups. Meanwhile, peripheral blood mononuclear cells(PBMCs)were collected and their expressions were compared in these two groups. PBMCs isolated from healthy subjects were cultured with 10 mmol/L glucose or 33.3 mmol/l glucose, respectively. The levels of STX8 expression and inflammatory cytokines were detected. Results The levels of body mass index(BMI), fasting blood glucose(FBG), fasting plasma insulin(FIN), total cholesterol(TC), trilycerides(TG), low-density lipoprotein cholesterol(LDL-C), free fatty acids(FFAs), homeostasis model assessment insulin resistence(HOMA-IR), tumor necrosis factor-α(TNF-α), interleukin 6(IL-6), STX8 in case group were significantly higher than that in control group, while HDL-C level in case group was significantly lower than that in control group(all P<0.05). The mRNA and protein expressions of STX8 in PBMCs of case group were significantly higher compared with that in control group(P<0.05). STX8 expression showed a positive correlation with FBG, FIN, TC, TG, LDL-C, FFAs, HOMA-IR, 山 东 大 学 学 报 (医 学 版)56卷12期 -陈攀,等.2型糖尿病患者外周血突触融合蛋白8表达与慢性炎症、糖脂代谢的相关性 \=-TNF-α, IL-6, while a negative correlation with HDL-C(all P<0.05). After treatment with high concentration, the levels of STX8 and TNF-α, IL-6 significantly increased, STX8 expression and TNF-α, IL-6 concentrations in 33.3 mmol/L glucose group was markedly higher than that in 10 mmol/L glucose group(all P<0.05). Conclusion STX8 level may be correlated with chronic inflammatory state and perturbed glucose and lipid metabolism in T2DM patients.

Key words: Type 2 diabetes mellitus, Syntaxin 8, Inflammation, Glucose and lipid metabolism

CLC Number: 

  • R587.1
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