JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 39-43.doi: 10.6040/j.issn.1671-7554.0.2016.200

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Regulatory role of KIF4A in the expression of angiogenesis- associated factors of macrophages

CHEN Yikun1, WANG Ketao2, WANG Huayang3, SHAO Qianqian3, TAN Wanye2, SONG Xiaobin2, QU Xun3, WEI Fengcai2   

  1. 1. Department of Burn &
    Plastic Surgery;
    2. Department of Oral and Maxillofacial Surgery;
    3. Institute of Basic Medical Sciences, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-02-29 Online:2017-01-10 Published:2017-01-10

Abstract: Objective To explore the effect of KIF4A on expressions of angiogenesis-associated factors in M0, M1 and M2 cells. Methods THP-1 cells were differentiated into M0, M1 and M2 cells under the treatment of PMA and different recombination human cytokines in vitro. The expressions of several angiogenesis-associated factors in these three macrophage subpopulations were analyzed by ELISA. KIF4A siRNA was transfected into M0, M1 and M2 cells, and the levels of angiogenesis-associated factors after the transfection were analyzed by qRT-PCR. Results LIF, VEGF, sVEGFR1 and TIMP1 were detected from supernatant of M0 cells, whereas the expressions of sVEGFR2, Flt-3, Leptin, LeptinR and CD40L were not detected. The expressions of LIF, VEGF and TIMP1 did not significantly change during the differentiation from M0 to M1 or M2 cells(P>0.05). The expression of sVEGFR1 was also constant in M0 and M1 cells(P>0.05), but its expression was significantly up-regulated in M2 cells (P<0.01). After the transfection of KIF4A siRNA, the levels of LIF, sVEGFR1, VEGF and TIMP1 did not significantly change in M0 and M1 cells, and LIF, VEGF and TIMP1 in M2 cells(P>0.05). However, sVEGFR1 expression in M2 cells was significantly decreased after KIF4A siRNA transfection(P<0.05). Conclusion M2 cells exhibite higher sVEGFR1 expression and KIF4A is involved in sVEGFR1 expression in M2 cells.

Key words: Angiogenesis-associated factors, Macrophages, Transfection, KIF4A

CLC Number: 

  • R392
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