JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 47-52.doi: 10.6040/j.issn.1671-7554.0.2015.019

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Establishment of rat models of rheumatoid arthritis induced by Borrelia burgdorferi

WANG Weiyan1, YANG Qingrui1, SHI Jianmin2, ZHAO Lulian2, ZHANG Yuanchao1   

  1. 1. Department of Rheumatology and Immunology; Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Parasitology and Tropical Medicine, National Defense Medical Center, Taipei 114, Taiwan, China
  • Received:2015-01-06 Online:2015-09-10 Published:2015-09-10
  • Contact: 张源潮。E-mail:qryang720@163.com E-mail:qryang720@163.com

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Abstract: Objective To establish rat models of rheumatoid arthritis by primary intradermal and secondary intraperitoneal injections of whole spirochaeta protein of Borrelia burgdorferi (B.b), and to explore the pathogenesis of the disease. Methods A total of 54 Lewis rats were randomly divided into low-dose B.b group, medium-dose B.b group, high-dose B.b group, complete Freunds adjuvant group, incomplete Freunds adjuvant group and PBS group, with 9 rats in each group. The degree of pathogenicity and pathological characteristics were assayed with arthritis index and histiopathological techniques. Serum cytokines were measured with enzyme linked immunosorbent assay (ELISA). Results The incidence of arthritis in high-dose B.b group was 66.7%. Histopathological tests showed typical arthritis pathology, including synovial proliferation, articular cartilage and bone erosion. The arthritis index, histological score and cytokine expressions of the high-dose B.b group had no statistical difference compared with those of the complete Freunds adjuvant group (P>0.05), but they were significantly higher than those of the PBS group (P<0.05). Conclusion Arthritis induced by whole spirochaeta protein of B.b mirrors human rheumatoid arthritis and is one of the ideal animal models for studying rheumatoid arthritis.

Key words: Animal, Borrelia burgdorferi, Model, Whole spirochaeta protein, Rheumatoid, Arthritis, Autoimmunity

CLC Number: 

  • R593.22
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