Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (11): 1-7.doi: 10.6040/j.issn.1671-7554.0.2025.0529

• Preclinical Medicine •    

Active peptide SK-14 derived from snail mucus promotes fibroblast proliferation and migration

LI Guanqiang1, SHI Yucheng1, ZHU Kehan2, HU Bo1, HUANG Xianchen1, SUN Yuan1, LI Duxin2, ZHANG Xicheng1   

  1. 1. Department of Vascular Surgery and Intervention, The Fourth Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu, China;
    2. College of Pharmaceutical Sciences, Soochow University, Suzhou 215000, Jiangsu, China
  • Published:2025-11-28

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Abstract: Objective To screen and synthesize the active peptide sequence, and to evaluate its effects on the proliferation and migration of fibroblasts based on the isolation, enzymatic digestion and sequencing of snail mucus. Methods Snail mucus protein was extracted and digested with trypsin for 4 h. The peptide sequence with proliferation promotion was screened by liquid chromatography-mass spectrometry and bioinformatics analysis, and then prepared by solid phase synthesis. Human adventitial fibroblasts(HAFs)were cultured and treated with different concentrations of active peptides to evaluate their effects on cell proliferation and migration. Results The synthesized peptide sequence was SK-14(N-terminal sequence: SSTIFPPTSGGAAK), with a molecular weight of 1,268 Da. Compared with the control group, SK-14 could significantly promote fibroblast proliferation at different concentrations(P<0.001). The cell proliferation ability increased in a concentration-dependent manner with the increase of SK-14 concentration. Within the test concentration range, the proliferation ability significantly increased with the increase of drug SK-14 concentration, and reached the plateau at 500 μg/mL. SK-14 can also significantly promote the migration ability of HAFs(P<0.001), and its effect increased with the increase of SK-14 concentration, with the most obvious effect at 1,000 μg/mL in the test concentration range. Conclusion The active peptide SK-14 derived from snail mucus can promote the proliferation and migration of HAFs, which may be one of the active components of snail mucus in promoting wound healing.

Key words: Snail mucus, Active peptides, Fibroblasts, Proliferation, Migration

CLC Number: 

  • R654.4
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