Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (1): 35-42.doi: 10.6040/j.issn.1671-7554.0.2024.0570

• Clinical Research • Previous Articles    

Expression of circ_0000144 in breast cancer and its effect on the proliferation, migration and invasion ability of breast cancer cells

ZHANG Jie1, ZHANG Fangfang1, WANG Jingnan2, LI Zeyu1, SONG Ying1, LI Na1   

  1. 1. Department of Pathology, Xinxiang Medical University, Xinxiang 453003, Henan, China;
    2. Sanquan College of Xinxiang Medical University, Xinxiang 453003, Henan, China
  • Published:2025-02-20

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Abstract: Objective To explore the expression of circ_0000144 in breast cancer tissues and its effect on the proliferation, apoptosis, migration and invasion abilities of breast cancer cells. Methods Quantitative real-time polymerase chain reaction(RT-qPCR)experiments were performed to detect the expression level of circ_0000144 in 49 breast cancer tissues and paired paracancerous normal breast tissue specimens and to analyze the relationship between the expression level of circ_0000144 and the clinicopathological characteristics of breast cancer patients. Normal breast epithelial cells MCF-10A were used as the control, and circ_0000144 expression was detected in breast cancer cell lines(T47D, MCF-7, MDA-MB-231)by RT-qPCR. MCF-7 cells were used as the study object, and the transfected si-circ_0000144 group was set up as the experimental group(si-circ_0000144 group), the transfected si-NC group was set as the saline control group(si-NC group), and a blank control group(Control group)was set at the same time. The cells were cultured with conventional medium without any transfection operation. The abilities of cell proliferation, migration and invasion in breast cancer cells were detected by CCK-8, clone formation assay, flow cytometry, scratch assay and Transwell assay, and the protein expressions of CyclinD1, p21, Bax, Bcl-2, E-cadherin and N-cadherin were detected by Western blotting. Results The expression of circ_0000144 in breast cancer tissues was significantly elevated compared with that in normal tissues adjacent to the cancer(P<0.001), and was closely positively associated with TNM stage and lymph node metastasis(P=0.003, P=0.007). The expressions of circ_0000144 were up-regulated in breast cancer cell lines compared to normal breast epithelial cells MCF-10A(P<0.001). Compared with the Control group or si-NC group, the si-Circ_0000144 group showed reduced abilities of proliferate, migrate and invade(all P<0.001), elevated apoptosis rate(both P<0.001), decreased expression levels of CyclinD1, Bcl-2 and N-cadherin proteins(all P<0.001), and increased expression levels of p21, Bax and E-cadherin proteins(all P<0.001). Conclusion circ_0000144 is highly expressed in breast cancer tissues and cell lines. Down-regulation of circ_0000144 can hinder breast cancer cell proliferation, migration and invasion, and promote its apoptosis. circ_0000144 may become a molecular target for breast cancer treatment.

Key words: Breast cancer, circ_0000144, Proliferation, Apoptosis, Migration, Invasion

CLC Number: 

  • R730
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