Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (5): 1-10.doi: 10.6040/j.issn.1671-7554.0.2023.0001

• 基础医学 •    

Effects of Xiaozheng Yiai decoction on the proliferation and migration of ovarian cancer SKOV3 cells

HE Jing1, YAN Rugen2, WU Zhihong3, LI Changzhong4,5   

  1. 1. The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China;
    2. School of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China;
    3. Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China;
    5. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China
  • Published:2023-05-15

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Abstract: Objective To investigate the effects of Xiaozheng Yiai decoction on the proliferation and migration of ovarian cancer SKOV3 cells and to explore the underlying mechanism. Methods The key active components and core targets of the decoction against ovarian cancer were analyzed with network pharmacology, and the related biological processes and pathways were screened with gene ontology(GO)and Kyoto Encyclopedia of genes and genomes(KEGG)enrichment analysis. Serum containing Xiaozheng Yiai decoction was prepared and SKOV3 cells were treated with it. Its effects on the proliferation of SKOV3 cells was detected with CCK-8 and EdU staining; its effects on the migration of SKOV3 cells was determined with wound healing assay and Transwell migration assay; its effects on the autophagy protein(P62), autophagy-related protein 3(ATG3), microtubule-associated protein 1 light chain 3 Beta(LC3B)and adenosine 5-monophosphate-activated protein kinase(AMPK)/ protein kinase B(AKT)/ mammalian target of rapamycin(mTOR)pathway related proteins in SKOV3 cells were assessed with Western blotting. Results Network pharmacology studies showed that the key active components of Xiaozheng Yiai decoction against ovarian cancer were quercetin, kaempferol, luteolin, wogonin, naringenin, and the core targets were MAPK3, AKT1, SRC, MAPK1, TP53, and so on. KEGG enrichment analysis identified 171 pathways(P<0.01). The pathways with the highest Degree ranking and associated with ovarian cancer mainly involved the cancer pathway, PI3K-AKT pathway, MAPK pathway, AMPK pathway, autophagy, and mTOR pathway. The results of in vitro experiments showed that compared with the control group, the 10% and 20% drug-containing serum groups had decreased cell viability, EdU positive cell rate, cell migration, and number of migrating cells(all P<0.001). The results of Western blotting showed that the protein expression levels of P62, ATG3 and LC3B-II were up-regulated after treatment with 10% and 20% drug-containing serum(all P<0.05), the p-AMPK/AMPK ratio was up-regulated(P<0.001), and the expression levels of p-AKT/AKT and p-mTOR/mTOR decreased (P<0.05 or P<0.01). Conclusion Xiaozheng Yiai decoction can inhibit the proliferation and migration of ovarian cancer SKOV3 cells, possibly via the regulation of AMPK/AKT/mTOR pathway and the blocking of autophagic flux.

Key words: Xiaozheng Yiai decoction, Ovarian cancer, Proliferation, Migration, Autophagy, Adenosine 5-monophosphate-activated protein kinase / protein kinase B / mammalian target of rapamycin

CLC Number: 

  • R737.31
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