Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 56-62.doi: 10.6040/j.issn.1671-7554.0.2020.1475

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Effects of domestic white light-emitting diodes exposure on rat retina

NAN Li1, YANG Kaizhuan2, ZHANG Yifan1   

  1. 1. Tianjin Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China;
    2. Department of Ophthalmology, Zhengzhou Second Hospital, Zhengzhou 450006, Henan, China
  • Published:2021-04-30

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Abstract: Objective To investigate the effects of different illumination levels of white light-emitting diodes(LED)light on the retinal structure and function of SD rats, in order to seek a relative safe illumination threshold. Methods A total of 40 rats aged 8-10 weeks were selected and randomly divided into control group and experimental groups(4 subgroups)with 8 animals in each group. After dark adaptation, the SD rats were exposed to 200, 300, 500 and 1 000 lux white LED light respectively in 12 hrs cycle for 7 days. After dark adaptation for 24 hours, the retinal function was evaluated with flash electroretinogram(F-ERG), and the histopathological changes were assessed with hematoxylin-eosin(HE)staining and transmission electron microscopy. Apoptotic cell death was evaluated with reverse transcription-polymerase chain reaction(RT-PCR)via the relative mRNA expressions of Bax, Bcl-2 and Caspase-3. Results Scotopic ERG showed that 500 and 1 000 lux white LED exposure resulted in a decrease in Rod-Rb, Max-Ra, Max-Rb and Ops. In the 1 000 lux group, HE staining revealed massive retinal damage. In the 500 and 1 000 lux groups, transmission electron microscopy showed cell membrane structure damage. RT-PCR showed the relative mRNA expressions of Bax and Caspase-3 increased and the mRNA expression of Bcl-2 decreased after 500 and 1 000 lux irradiation. Conclusion Under daily indoor lighting intensity, 7-day cycle of white LED irradiation has no significant effects on the retinal structure and function of SD rats. Light intensity up to 500 lux can cause retinal photodamage in SD rats in a dose-dependent manner. The apoptosis-related genes Bax and Caspase-3 promote the occurrence and progression of retinal light damage.

Key words: White light, Light-emitting diodes, Retina, Apoptosis, SD rat

CLC Number: 

  • R774.1+3
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