Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 68-74.doi: 10.6040/j.issn.1671-7554.0.2018.1443

Previous Articles    

Effect of ar-turmerone on poliferation, migration and invasion of cervical squamous cancer cell line SiHa

LUO Mengying1, WU Bei2, WANG Ye1, LUO Heng3,4, CAO Yu1   

  1. 1. Guizhou Medical University, Guiyang 550004, Guizhou, China;
    2. Department of Gynecology and Obstetrics, the 44 Hospital of Peoples Liberation Army, Guiyang 550009, Guizhou, China;
    3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang 550014, Guizhou, China;
    4. Key Laboratory of Chemistry for Natural Products of Guizhou Province, Chinese Academy of Sciences, Guiyang 550014, Guizhou, China
  • Published:2022-09-27

PDF (PC)

5

Abstract: Objective To investigate the effect of ar-turmerone on the proliferation, migration and invasion of cervical cancer SiHa cells in vitro, and to preliminarily explore its mechanism. Methods SiHa cells were incubated with different concentrations of ar-turmerone(0, 20, 40, 80 mg/L), and cell proliferation was measured by CCK8. The apoptosis was observed by giemsa staining. The effects of ar-turmerone on migration and invasion capacity of SiHa cells were detected by cell scratch assay and transwell. The apoptosis was analyzed by flow cytometry. The expressions of MMP2, MMP9 and p53 protein in SiHa cells were detected by Western blotting. Results Ar-turmerone dose-dependently inhibited SiHa cells proliferation(Fconcentration=162.419, P<0.001; Ftime=99.442, P<0.001; Fconcentration×time=2.111, P=0.095), migration(Fconcentration=432.158, P<0.001; Ftime=528.845, P<0.001)and invasion(Fconcentration=434.362, P<0.001). 山 东 大 学 学 报 (医 学 版)57卷6期 -罗梦颖,等.芳姜黄酮对SiHa细胞增殖、迁移及侵袭的影响 \=-Flow cytometry analysis showed that ar-turmerone could induce the apoptosis of SiHa cells in a dose-dependent manner(Fconcentration=482.690, P<0.001). Western blotting results showed that ar-turmerone treatment significantly up-regulated the expression of apoptosis-related proteins p53(Fconcentration=2.086, P=0.045)and down-regulated the expressions of invasion and metastasis related proteins MMP2 and MMP9(Fconcentration=18.906, P=0.001; Fconcentration=9.847, P=0.005). Conclusion Ar-turmerone has the ability to inhibit the proliferation and induce apoptosis of SiHa cells in vitro, and the mechanism is related to the regulation of apoptosis related protein p53 expression. In addition, ar-turmerone can inhibit the migration and invasion of SiHa cells, possibly through the regulation of the expressions of MMP2 and MMP9 protein.

Key words: Ar-turmerone, SiHa cell, Apoptosis, Migration, Invasion

CLC Number: 

  • R285.5
[1] Peralta-Zaragoza O, Deas J, Meneses-Acosta A, et al. Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells[J]. BMC Cancer, 2016, 16: 215. doi:10.1186/s12885-016-2231-3.
[2] Zheng W, Liu Z, Zhang W, et al. miR-31 functions as an oncogene in cervical cancer[J]. Arch Gynecol Obstet, 2015, 292(5): 1083-1089.
[3] Chen Y, Wang H, Lin W, et al. ADAR1 overexpression is associated with cervical cancer progression and angiogenesis[J]. Diagn Pathol, 2017, 12(1): 12. doi:10.1186/s13000-017-0600-0.
[4] Barra F, Lorusso D, Leone Roberti Maggiore U, et al. Investigational drugs for the treatment of cervical cancer[J]. Expert Opin Investig Drugs, 2017, 26(4): 389-402.
[5] Brucker SY, Ulrich UA. Surgical treatment of early-stage cervical cancer[J]. Oncol Res Treat, 2016, 39(9): 508-514.
[6] Liu C, Liao JZ, Li PY. Traditional Chinese herbal extracts inducing autophagy as a novel approach in therapy of nonalcoholic fatty liver disease[J]. World J Gastroenterol, 2017, 23(11): 1964-1973.
[7] Reis FS, Lima RT, Morales P, et al. Methanolic extract of Ganoderma lucidum induces autophagy of AGS human gastric tumor cells[J]. Molecules, 2015, 20(10): 17872-17882.
[8] Yu N, Xiong Y, Wang C. Bu-Zhong-Yi-Qi decoction, the water extract of Chinese traditional herbal medicine, enhances cisplatin cytotoxicity in A549/DDP cells through induction of apoptosis and autophagy[J]. Biomed Res Int, 2017, 2017: e3692797. doi:10.1155/2017/3692797.
[9] Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014[J]. J Nat Prod, 2016, 79(3): 629-661.
[10] 庞邦斌, 银胜高, 黄云兰, 等. 不同产地姜黄药材的质量研究[J]. 华西药学杂志, 2014, 29(3): 308-310. PANG Bangbin, YIN Shenggao, HUANG Yunlan, et al. Quality assessment on Curcuma longa from the different producing areas[J]. West China Journal of Pharmaceutical Sciences, 2014, 29(3): 308-310.
[11] Gounder DK, Lingamallu J. Comparison of chemical composition and antioxidant potential of volatile oil from fresh, dried and cured turmeric(Curcuma longa)rhizomes[J]. Industrial Crops and Products, 2012, 38: 124-131. doi:10.1016/j.indcrop.2012.01.014.
[12] Prakash P, Misra A, Surin WR, et al. Anti-platelet effects of Curcuma oil in experimental models of myocardial ischemia-reperfusion and thrombosis[J]. Thromb Res, 2011, 127(2): 111-118.
[13] Kim D, Suh Y, Lee H, et al. Immune activation and antitumor response of ar-turmerone on P388D1 lymphoblast cell implanted tumors[J]. Int J Mol Med, 2013, 31(2): 386-392.
[14] Tundis R, Xiao J, Loizzo MR. Annona species(Annonaceae): a rich source of potential antitumor agents?[J]. Ann N Y Acad Sci, 2017, 1398(1): 30-36.
[15] Zhang X, Wang R, Chen G, et al. The effects of curcumin-based compounds on proliferation and cell death in cervical cancer cells[J]. Anticancer Res, 2015, 35(10): 5293-5298.
[16] Shang HS, Chang CH, Chou YR, et al. Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells[J]. Oncol Rep, 2016, 36(4): 2207-2215.
[17] Joshi JV, Jagtap SS, Paradkar PH, et al. Cytologic follow up of Low-grade Squamous Intraepithelial Lesions in Pap smears after integrated treatment with antimicrobials followed by oral turmeric oil extract[J]. J Ayurveda Integr Med, 2016, 7(2): 109-112.
[18] Forbes-Hernández TY, Giampieri F, Gasparrini M, et al. The effects of bioactive compounds from plant foods on mitochondrial function: a focus on apoptotic mechanisms[J]. Food Chem Toxicol, 2014, 68: 154-182. doi:10.1016/j.fct.2014.03.017.
[19] Tuzlak S, Kaufmann T, Villunger A. Interrogating the relevance of mitochondrial apoptosis for vertebrate development and postnatal tissue homeostasis[J]. Genes Dev, 2016, 30(19): 2133-2151.
[20] Wu CC, Bratton SB. Regulation of the intrinsic apoptosis pathway by reactive oxygen species[J]. Antioxid Redox Signal, 2013, 19(6): 546-558.
[21] Thornborrow EC, Patel S, Mastropietro AE, et al. A conserved intronic response element mediates direct p53-dependent transcriptional activation of both the human and murine bax genes[J]. Oncogene, 2002, 21(7): 990-999.
[22] Wu Y, Mehew JW, Heckman CA, et al. Negative regulation of Bcl-2 expression by p53 in hematopoietic cells[J]. Oncogene, 2001, 20(2): 240-251.
[23] Chen YR, Tan TH. Lack of correlation in JNK activation and p53-dependent Fas expression induced by apoptotic stimuli[J]. Biochem Biophys Res Commun, 1999, 256(3): 595-599.
[24] Gough MJ, Killeen N, Weinberg AD. Targeting macrophages in the tumour environment to enhance the efficacy of αOX40 therapy[J]. Immunology, 2012, 136(4): 437-447.
[25] Chen K, Zhang S, Ji Y, et al. Baicalein inhibits the invasion and metastatic capabilities of hepatocellular carcinoma cells via down-regulation of the ERK pathway[J]. PLoS One, 2013, 8(9): e72927. doi:10.1371/journal.pone.0072927.
[26] Shuman Moss LA, Jensen-Taubman S, Stetler-Stevenson WG. Matrix metalloproteinases: changing roles in tumor progression and metastasis[J]. Am J Pathol, 2012, 181(6): 1895-1899.
[27] Littlepage LE, Sternlicht MD, Rougier N, et al. Matrix metalloproteinases contribute distinct roles in neuroendocrine prostate carcinogenesis, metastasis, and angiogenesis progression[J]. Cancer Res, 2010, 70(6): 2224-2234.
[1] WANG Wei, WANG Yi-Feng, ZHANG Ling-Mei, LIN Qiong-Yan, HUANG Ju. umorigenicity and invasion of side population in human ovarian epithelial cancer OVCAR3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 8-11.
[2] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[3] ZHAO Ge, ZOU Cunhua, SONG Dongdong, ZHAO Shuping. Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 53-58.
[4] ZHANG Zhenwei, LI Jia, CHEN Keming. IGF2BP2/m6A/ITGA5 signal axis regulates the proliferation and migration of renal clear cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 74-84.
[5] SUN Lina, DU Xiaoxiao, ZHANG Hongjuan, MENG Jinlai. Human leukocyte antigen-G promotes trophoblast invasion by regulating decidual natural killer cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(6): 41-45.
[6] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
[7] CHEN Zhaobo, FANG Min, XUE Haoran, LIU Chunyan. Deubiquitinase USP35 promotes the cells migration and invasion of non-small cell lung cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 30-37.
[8] ZHONG Lili, SHENG Ying, GUO Jianghong, YANG Shuangjian, HE Yijing. LncRNA-UCA1 effects invasion and metastasis of trophoblast cells by targeting miR-182-5p [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 76-82.
[9] ZUO Liping, JIANG Fengyang, ZHOU Binbin, FAN Jinlei, LIANG Yongfeng, DENG Zhanhao, YU Dexin. Value of preoperative multiphase MRI for predicting microvascular invasion and early recurrence of 169 hepatocellular carcinoma [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 89-95.
[10] LI Hui, JIANG Chaoyang, LIU Yan, ZHANG Man. Effects and mechanism of histone deacetylase SIRT1 controlled macrophage apoptosis induced by oxidized low density lipoprotein [J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 6-12.
[11] LU You, QIE Di, WU Jinhui, YANG Fan. Effects of the intervention of Sonic Hedgehog signaling pathway on learning and memory ability in rats with intrauterine growth restriction [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 82-89.
[12] NAN Li, YANG Kaizhuan, ZHANG Yifan. Effects of domestic white light-emitting diodes exposure on rat retina [J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 56-62.
[13] KONG Xue, LI Juan, DUAN Weili, SHI Shuang, LI Peilong, DU Lutao, MAO Haiting, WANG Chuanxin. Effects of lncRNA AC012073.1 on the migration and invasion of human breast cancer cells and its clinical significance [J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 70-78.
[14] LIU Shudan, ZHANG Feiyan, GUO Songlin, LIANG Xueyun, CHEN Dongmei. Oxymatrine ameliorates oxidative stress injury of HaCaT cells induced by hypoxia ischemia [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 26-34.
[15] XUE Yuan, LIN Xueyan, XU Ge, TIAN Yongjie. Expression of hypoxia-inducible factor-1α in the serum of patients with endometriosis and its effect on epithelial mesenchymal transition of eutopic endometrial stromal cells [J]. Journal of Shandong University (Health Sciences), 2021, 59(2): 41-47.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd