Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 61-67.doi: 10.6040/j.issn.1671-7554.0.2019.426

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Expressions of apelin, nucleobindin 2 and retinoic acid receptor responder protein 2 in glioblastoma based on network databases

KONG Juan, ZHANG Zhen, GUO Wei, HAN Ping   

  1. Intensive Care Unit Of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the expressions and significances of adipokines apelin(APLN), nucleobindin 2(NUCB2)and retinoic acid receptor responder protein 2(RARRES2)in glioblastoma multiforme(GBM). Methods The mRNA expressions of APLN, NUCB2 and RARRES2 in GBM tissues and control tissues, and the correlations between them and vascular endothelial growth factor A(VEGFA), CD34 and matrix metalloproteinase 14(MMP14)were determined with GEPIA. The relationship between APLN, NUCB2 and RARRES2 expressions and GBM prognosis was analyzed with GEPIA and Survexpress. The mRNA expressions of APLN and its receptor, NUCB2, RARRES2 and its receptors in 4 glioma cell lines were analyzed with cancer cell line encyclopedia(CCLE). Results The mRNA expressions of APLN, NUCB2 and RARRES2 were significantly up-regulated in GBM patients (P<0.05); APLN was associated with VEGFA (R=0.52, P<0.05) and CD34 (R=0.39, P<0.05); NUCB2 was associated with MMP14 (R=0.43, P<0.05); The expression of RARRES2 was negatively correlated with the prognosis of GBM(GEPIA: Log rank P=0.027; Survexpress: Log rank P=0.007). NUCB2 was highly expressed in all 4 cell lines. The APLN receptor was expressed the highest in A172 cells. The RARRES2 receptor was expressed the highest in U87MG cells. Conclusion APLN, NUCB2 and RARRES2 are highly expressed in GBM tissues. APLN is closely related to GBM angiogenesis. NUCB2 may link to GBM invasion and RARRES2 is associated with poor prognosis.

Key words: Glioblastoma multiforme, Apelin, Nucleobindin 2, Retinoic acid receptor responder protein 2, Data analysis

CLC Number: 

  • R739.4
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