Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (7): 7-14.doi: 10.6040/j.issn.1671-7554.0.2017.929

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Effects of Fusobacterium nucleatum gavage on the intestinal microbiota of rats

SONG Lijin, GU Xiang, LI Lixiang, LI Ming, ZUO Xiuli, LI Yanqing   

  1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of Fusobacterium nucleatum(F. nucleatum)gavage on the intestinal microbiota of rats. Methods A total of 16 male SD rats were randomly divided into 2 groups: intervention group(n=8)and control group(n=8). The intervention group was treated with F. nucleatum when the rats were 4, 5, 6, 7 and 8 weeks old. The control group was treated with the same dosage of normal saline at the same time. Feces was collected at the end of week 3, 8 and 12 to extract DNA, and then Illumina Miseq platform was used for 16S rRNA gene sequencing. Specific IgA against F. nucleatum in stool was detected with Western blotting. Results There were no significant differences between the two groups in terms of fecal microbial diversity and relative abundance of main phyla in week 3. The microbial diversity of the intervention group was significantly lower than that of the control group in week 8(P=0.009). The relative abundances of Firmicutes and Proteobacteriodes in the intervention group were significantly less than those in the control group(P<0.05), while the relative abundance of Bacteriodes in the intervention group was significantly higher than that in the control group in week 8(P<0.05). In week 12, the intervention group showed higher diversity(P=0.021), and there was no significant difference in the relative abundance of main phyla. Moreover, the treatment of F. nucleatum stimulated the production of specific IgA. Conclusion The F. nucleatum can interfere with intestinal microbiota independent on colonization, and this effect can last for some time. The production of specific IgA induced by F. nucleatum may explain why this bacterium does not colonize in the intestine.

Key words: Fusobacterium nucleatum, Intestinal microbiota, High-throughput sequencing, Rats

CLC Number: 

  • R574
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