JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (2): 19-21.doi: 10.6040/j.issn.1671-7554.0.2014.533

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Effect of Glucagon-like peptide-1 on ovarian circadian clock rhythm of rats fed with high fat diet

YAO Zhina, HAN Ting, FAN Xiuling, ZHANG Yousheng, SONG Jialun   

  1. Department of Gynecology, Center for Reproductive Medicine, Shandong University, Jinan 250021, Shandong, China
  • Received:2014-08-13 Online:2015-02-10 Published:2015-02-10

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Abstract: Objective To explore the therapeutic effect of glucagon-like peptide-1(GLP-1) on impaired circadian gene of ovary in rats fed with high-fat diet. Methods Wistar rats were randomly divided into control group, high-fat diet group and GLP-1 intervention group. Eight weeks later, the levels of fasting glucose, insulin, cholesterol, triglyceride, E2, FSH and LH were tested. The expression of ovary related gene and circadian gene in ovary tissues were detected with Real-time PCR. Results GLP-1 had certain effect on serum biochemical indexes in rats fed with high fat. Real-time PCR results showed that, compared with control group, the levels of primordial follicle recruitment factor anti-mullerian homone (AMH) and growth differentiation factor (GDF-9) were significantly decreased in rats fed with high-fat diet (P<0.05). However, the expression of AMH and GDF-9 mRNA in high-fat diet rats treated with GLP-1 were markedly elevated. Besides, compared with control group, the expression of ovary circadian clock related genes was affected in rats fed with high-fat diet, and GLP-1 could improve the expression of ovary circadian gene. Conclusion GLP-1 could ameliorate the expression of ovary circadian gene in rats fed with high-fat diet.

Key words: Circadian clock, High-fat feeding, Glucagon-like peptide-1, Ovary, Rats

CLC Number: 

  • R711.6
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