JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (1): 37-41.doi: 10.6040/j.issn.1671-7554.0.2013.587

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Effects of bone marrow mesenchymal stem cells transplantation on the expression of  Toll-like receptor 4 in injured spinal cord of rats

MOU Le-ming1， SUN Zhan-sheng1， WANG Bo-min1， GAO Ping2， CHU-Xiang-quan3   

  1. 1. Department of Traumatic Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
    2. Department of Joint Surgery, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    3. Department of Traumatic Orthopedics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
  • Received:2013-09-29 Online:2014-01-10 Published:2014-01-10

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Abstract:

ObjectiveTo investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of Toll-like receptor 4 (TLR4) in injured spinal cord of rats, and to explore the mechanism by which BMSCs transplantation treats spinal cord injury. Methods  A 4-week old mouse was utilized to separate BMSCs, which were cultured into third generation and then identified by immunohistochemical Methods  . A total of 90 8-week old male Wistar rats were evenly divided into three groups: group of surgical control, group of spinal cord injury and group of cell transplantation. Rats in group of spinal cord injury was used to establish Allen′s SCI model, rats in group of cell transplantation was injected with the BMSCs suspention after SCI. Then the rats were killed respectively at 24 hours, 72 hours, 7 days and 1 cm of the spinal cord blocks in the center of contusion site was removed. The expression of TLR4 in the injured area was detected with immunohistochemical method and real-time quantitative PCR (RT-qPCR). Results  The expression of TLR4 in group of spinal cord injury increased compared with that of group of surgical control (P<0.01). The expression of TLR4 in group of cell transplantation decreased compared with that of group of spinal cord injury (P<0.01). Conclusion  BMSCs transplantation may inhibit inflammation in spinal cord injury by decreasing the expression of TLR4.

Key words: BMSCs, Cell transplantation, Spinal cord injury, Toll-like receptor 4, Rats

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