JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

The MAGE-3-sensitized dendritic cell vaccine kills bladder cancer cells in vitro

MA Ming 1,2, LI Xiu-zhen3, WANG Chao1, GUO Hong-min2, MA Xin1, ZHANG Xu1   

  1. 1. Department of Urology, Tongji Hospital,Huazhong University of Science and Technology, Wuhan 470030, China;2. Department of Urology, Jining First People′s Hospital, Jining 272011, China;3. Department of Microbiology, Jining Medical College, Jining 272011, Shandong, China
  • Received:2008-01-17 Revised:1900-01-01 Online:2008-05-16 Published:2008-05-16
  • Contact: ZHANG Xu

PDF (PC)

418

Abstract: To explore the cytotoxic activity of cytotoxicity T lymphocyte (CTL) induced by MAGE-3-sensitized dendritic cells (DCs) on the bladder cancer cell line BIU-87. MethodsProliferation of T cells and cytotoxic activity of CTL were determined by the MTT method. CTL was induced by MAGE-3-sensitized DC cells, and ovalbumin (OVA) peptide and un-sensitized DC served as controls. ResultsThe MAGE-3-sensitized DC could promote proliferation of T cells and cytotoxity activity of the CTL cells on bladder cancer cells as compared with OVA peptide or unsensitized DC. ConclusionDC sensitized by the MAGE-3 antigen peptide can promote multiplication of T cells and can develop a cell immunity function, it also inhibits the growth of tumor and accelerates tumor decline. It can be used as a novel vaccine on bladder cancer therapy.

Key words: Bladder neoplasms, Dendritic cells, Antigen, Peptides, MAGE-3 Gens

CLC Number: 

  • R737.14
[1] WANG Yao, CUI Guoying, WANG Fei, LI Changzhong. Expression and significance of human leucocyte antigen-E in cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 59-63.
[2] GONG Yanjie, WANG Long, DONG Laidong, LI Dong, SONG Guangmin, JU Xiuli. Effects of different cryoprotectants and cooling methods on the histology and immunogenicity of heart valves [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 44-49.
[3] ZHENG Min, ZHANG Lan. Current situation and development trend of CAR-T in anti-tumor research [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 1-6.
[4] YANG Hui, DONG Rong, XUE Hui, HU Zuquan, ZENG Zhu. Gene microarray analysis of mature dendritic cells cultured with hepatocellular carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 1-6.
[5] WANG Jinghan, LI Xiaofei, ZHANG Zhibing, WANG Haibo, LI Jianfeng. Prestin expression in outer hair cells of Spag6 gene knockout mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 16-22.
[6] GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong. Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 41-45.
[7] JIANG Xuewen, CHEN Jun, ZHANG Zhaocun, ZHU Yaofeng, MENG Hui, SHI Benkang. Clinical characteristics of prostatic cystadenoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 81-86.
[8] DING Juan, ZHENG Guixi, DU Lutao, YIN Zuohua, ZHANG Jian, WANG Chuanxin. Evaluation of combined detection of sHLA-G, PGR and GPR in the diagnosis of gastric cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(11): 59-63.
[9] ZHANG Lu, YAN Lei, ZHANG Hui, ZHANG Xiaohui, LI Mingjiang, ZHAO Xingbo. Expression and clinical significance of sperm-associated antigen 9 in patients with cervical cancer and cervical intraepithelial neoplasia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 67-71.
[10] ZHAO Hua1, XU Yangyang2, LU Xiangdong3， XU Tongjiang1. Correlation among Ki-67, PTTG, VEGF and pituitary apoplexy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 82-85.
[11] FANG Min1, BI Ke-hong2, JIANG Guo-sheng3, ZHU Chuan-sheng2. Changes of the LMP/TAP gene expressions and its significance in leukemia cells HL-60 after induced differentiation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(2): 1-5.
[12] ZHUANG Yong, LI Dong, FU Jinqiu, SHI Qing, JU Xiuli. Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 60-64.
[13] HAN Jie1, LIU Yi-qing1, REN Yan-yan2, LUAN De-heng1, GAO Kun1, XIE Feng-yang1. Changes of substance P and betaEndorphin in the brain of  rats with trigeminal neuralgia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 45-48.
[14] FU Xiao-li， WANG Lei， LIU Feng. HBsAg quantitative value in different stages of hepatitis B virus infection and its correlations with serum HBV DNA and age [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 94-98.
[15] ZHAO Ying， YAN Lei， ZHANG Hui， YU Peng， LI Ming-jiang， ZHAO Xing-bo. Expression of SPAG9 in patients with serous epithelial ovarian tumors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 98-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd