JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effects of the survivin　antisense oligonucleotide on survivin mRNA expression and chemosensitivity to Paclitaxel in human breast cancer cells MCF-7

HAN Fang, LI Li   

  1. Cancer Center， Qilu Hospital　of　Shandong University, Jinan 250012， China
  • Received:2007-08-13 Revised:1900-01-01 Online:2008-01-16 Published:2008-01-16
  • Contact: LI Li

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Abstract: To investigate the effects of the survivin antisense oligonucleotide(ASODN) on cell proliferation，apoptosis and chemosensitivity to paclitaxel in human breast cancer cells MCF-7. MethodsSurvivinASODN was transfected into MCF-7cells mediated by Lip reagent. The expression of survivin mRNA was determined by RT-PCR, cell proliferation and chemosensitivity to paclitaxel in MCF-7 were determined by MTT assay, and apoptosis and cell cycle were determined by flow cytometry. Results The antisense compound efficiently downregulated survivin mRNA expression in a timedependent manner. The growth inhibition rate of MCF-7 was decreased in a dose and timeindependent manner. The cell cycle was arrested at G2/M phase; apoptosis was obviously found. Paclitaxel could enhance the expression of survivin at an early time, and this effect might play a critical role in the escape to apoptosis induced by paclitaxel, which may promote the resistance of paclitaxel. The antisense compound efficiently downregulated this induction by paclitaxel, and enhanced the chemosensitivity to paclitaxel in MCF7. ConclusionSurvivin antisense oligonucleotide effectively suppresses the expression of survivin mRNA, increases apoptosis and enhances the chemosensitivity to paclitaxel in MCF-7.

Key words: Breast neoplasms, Survivin oligoribonucleotides, antisense, Cell proliferation, Apoptosis, Chemosensitivity

CLC Number: 

  • R737.9
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