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Comparison of two methods in the isolation of human placenta mesenchymal stem cells

LI Dong1，WANG Guo-yun2，JU Xiu-li1,SHI Qing1，HOU Huai-shui1   

  1. 1. Lab of Cryomedicine； 2. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2006-12-05 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
  • Contact: WANG Guo-yun

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Abstract: Objective: To investigate the protocols of isolating mesenchymal stem cells from the placenta and to evaluate their biological characters. Methods: The explant method and enzyme digestion method were used to isolate mesenchymal stem cells from the placenta. Morphology, immunotyping, growth kinetics and mutitopent differentiation of these cells were assayed. Results: FACS and differentiation experiments showed immunotyping of adherent cells isolated by the enzyme digestion method matched with the character of mesenchymal stem cells and could differentiate into osteoblasts, chondrocytes and adipocytes under an appropriate induction condition. Cells isolated by the explant method weakly expressed CD29 and CD105 and could differentiate only into adipocytes. Conclusion: The enzyme digestion method can effectively isolate mesenchymal stem cells with a high proliferative ability and multipotent differentiation ability.

Key words: Placenta, Stem cells, Mesoderm, Cell culture

CLC Number: 

  • R329
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