JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles •     Next Articles

Survival and differentiation of GFP transgenic mice-derived neuroepithelial cells after being transplanted into rat brains

WANG Jia-zeng， BAO Li-hua， SUN Jin-hao， LI Zhen-hua， ZHANG Jing   

  1. Department of Anatomy， School of Medicine， Shandong University， Jinan 250012， Shandong, China
  • Received:2007-01-05 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
  • Contact: SUN Jin-hao

PDF (PC)

447

Abstract: Objective: To observe the survival and differentiation of cells derived from the embryonic neural tube of green fluorescent protein (GFP) transgenic mice after being transplanted into the striatum of rats. Methods: Neuroepithelial stem cells derived from the embryonic neural tube of GFP transgenic mice were cultured and depurated in free serum medium containing B27, bFGF and EGF, and then they were directionally transplanted into the striatum of rats. Animals were sacrificed at different time points after being transplanted and frozen sectioned, and staining by MAP-2, GFAP, and TH was determined by immunohistochemical techniques. Results: Neuroepithelial stem cells derived from the embryonic neural tube could expand into neurospheres in the free serum medium and express nestin. The grafted regions were clearly visible, and the transplanted cells survived well and some of them differentiated into TH-positive cells. Conclusion: Embryonic neuroepithelial stem cells from GFP transgenic mice could be generated in vitro, survive and differentiate into neurons, especially dopaminergic neurons and neuroglia cells after being transplanted into rat brains.

Key words: Mice, transgenic, Neuroepithelium, Stem cells, Proliferation, Transplantation

CLC Number: 

  • R322.81
[1] DUAN Chu-Hong, LIU Kai, YIN Hai-Yan, ZHAO Shi-Dou, LIU Feng-Tao. Effects of lectin receptors of WGA,RCA and ECL on the development of cleft palate induced by excess retinoid acid in KM mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 47-.
[2] WANG Wei, WANG Yi-Feng, ZHANG Ling-Mei, LIN Qiong-Yan, HUANG Ju. umorigenicity and invasion of side population in human ovarian epithelial cancer OVCAR3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 8-11.
[3] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[4] ZHANG Hui, SHI Qing, HOU Huai-Shui, LI Dong. Culture of human cord mesenchymal stem cells using animal serum-free medium and hepatogenic differentiation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 88-.
[5] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[6] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[7] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[8] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38-46.
[9] LI Songlin, LIU Peilai, LU Qunshan, MA Heran. Application of high tibial osteotomy combined with autologous adipose-derived mesenchymal stem cells injection in the repair of knee cartilage [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 82-88.
[10] ZHAO Shanshan, LI Xiaomei, LIANG Ting, ZHANG Chao, SONG Jing, HOU Guihua. Impact of autophagy on targeting imaging of allorejection with 125I-anti-TLR5 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 46-52.
[11] WANG Kaimin, TAN Juanjuan, YAN Zhiqiang, LI Zhiqiang. Effects of Sirtuin on hypoxia-induced proliferation of human umbilical cord-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 23-30.
[12] LI Jingyuan, SONG Ling, LIN Song, SONG Hui. Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 31-37.
[13] JIAO Fengping, WANG Yu, YU Ailian, TIAN Zhaoju, YANG Shulin. Immunogenicity of the eukaryotic expression vector pcDNA3.1(-)-P6-gBCTL-TBK-1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 75-78.
[14] SHI Peikun, ZENG Beini, WU Weifang, HU Xiaoyan, MA Tianjia, ZHOU Yabin. Expression and role of Keap1 in renal cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 94-99.
[15] SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd