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Effect of tamoxifen on the proliferation and expression of ER and Smad4 in human hepatocellular carcinoma cells HepG2

BAI Wen-kun1，WANG Wen-qi2，SHI Chang-wen2，WU Hong-wen3   

  1. 1.Department of Gastroenterology, Qianfo Mountain Hospital of Shandong Province, Shandong University；2. Department of Gastroenterology, Qianfo Mountain Hospital of Shandong Province；3.Department of Gastroenterology
  • Received:2006-07-04 Revised:1900-01-01 Online:2007-06-24 Published:2007-06-24
  • Contact: WANG Wen-qi

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Abstract: Objective: To study the effect of tamoxifen on the proliferation and expression of the estrogen receptor(ER) and Smad4 in human hepatocellular carcinoma cells HepG2. Methods: Three experimental groups were subjected to 7.5μmol/L,　15μmol/L,　 and 30μmol/L tamoxifen. The control group was not subjected to tamoxifen. Each group contained six wells, and there were 0.1ml containing 1×105/ml cells in each well. After being observed for 24h, 48h, and 72h, the inhibiting rate of HepG2 cells was determined by MTT. The expression of ER and Smand4 in HepG2 cells was determined by immunohistochemistry. Results: Tamoxifen inhibited the proliferation of human hepatocellular carcinoma cells in a time and dose dependent manner. It also suppressed the ER expression but raised the Smad4 expression in a time and dose dependent manner. Conclusion: Tamoxifen can suppress human hepatocellular carcinoma cell proliferation by affecting the expression of ER and Smad4 in HepG2 cells.

Key words: Carcinoma, hepatocellular, Tamoxifen, Receptors, estrogen, Smad4

CLC Number: 

  • R735.7
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