JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effect of 2-methoxyestradiol on proliferation and apoptosis of neurons and C6 glioma cells in rats

ZHANG Hong-ri1，LI Gang1，ZHAO Yong-gang2，CHENG Xiao-bing2，PENG Jing-hua3   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University; 2. Department of Neurosurgery， First Affiliated Hospital; 3. Department of Neurology， First Affiliated Hospital, Henan University of Science and Technology
  • Received:2007-04-02 Revised:1900-01-01 Online:2007-06-24 Published:2007-06-24
  • Contact: LI Gang

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Abstract: Objective： To investigate the effect of 2-methoxyestradiol on growth inhibition and apoptosis of C6 glioma cells and neurons in vitro. Methods： C6 glioma cells and neurons were divided into the control group and four 2-methoxyestradiol treatment groups. C6 glioma cells and neurons were treated by different doses of 2-methoxyestradiol for different times and observed by microscopy and electromicroscopy. The viability was determined by MTT assay and the cellcycle and apoptosis were determined by flow cytometry. Results： 2-methoxyestradiol caused a dose and timedependent inhibition on the C6 glioma cells viability(P<0.05). The population of C6 glioma cells in the G1 and S phase was decreased while in the G2 phase it wasincreased by 2-methoxyestradiol in a dose and time-dependent manner(P<0.05); However, 2-methoxyestradiol had no effect on neurons. Conclusion： 2-methoxyesradiol can inhibit proliferation and induce apoptosis of C6 glioma cells, but has no effect on neurons.

Key words: 2-methoxyestradiol, Apoptosis, C6 glioma cell, Neurons

CLC Number: 

  • R739.4
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