JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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SiRNA mediated silencing of the epidermal growth factor receptor induces the apoptosis of HepG2

MOU Zhuang-bo1，ZHAO You-an1，WANG Yan-jun2   

  1. 1． Department of Gasteroenterology， Qilu Hospital of Shandong University；2． Central Laboratory， Shandong Provincial Hospital
  • Received:2006-11-06 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
  • Contact: MOU Zhuang-bo

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Abstract: Objective: To evaluate the RNAi efficiency of siRNA plasmid on the EGFR gene in a HepG2 cell line. Methods: By using a plasmid vector containing duplex of oligonucleotides, in which 19nt specific sequence of EGFR expressing cassette to induce RNAi, the expression of endogenous EGFR in HepG2 human hepatocarcinoma cells was suppressed. The HepG2 cells were transfected with pSIREN-hE consisting of a RNAi-ready pSIREN-shuttle vector and duplex of oligonucleotides, which were built up of 69nt base pair, into a 19nt specific sequence of EGFR expressing cassette that can be transcripted by promoter U6. Results: The mRNA of EGFR in HepG2 human hepatocarcinoma cells was decreased and cell proliferation was reduced due to the induction of apoptosis. Conclusion: siRNA-mediated inhibition of EGFR gene may be a useful approach in the treatment of human hepatocarcinoma.

Key words: Epidermal growth factor receptor, mRNA, RNA interference, Apoptosis

CLC Number: 

  • R735.7
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