JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

Effect of striatal extracts on inducing the NSC differentiate into dopaminergic neurons: an experimental study

WANG Xin-yu1，LI Xin-gang1，ZHOU Lu1，BAO Xiu-feng1，XIN Hua2   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, 2. Institute of Cytology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2007-05-22 Revised:1900-01-01 Online:2007-11-24 Published:2007-11-24
  • Contact: LI Xin-gang

PDF (PC)

640

Abstract: ObjectiveTo culture human fetal neural stem cells in vitro, and then induce them to differentiate into dopaminergic neurons. Methods Human fetal neural stem cells derived from the hippocampus were purified and induced by human fetal striatal extracts. The induced differentiational rate of dopaminergic neurons was detected by immunocytoflurescence and the expression of TH- mRNA was determined by RT-PCR. Results The rate of TH+ cells of the experimental group was higher than that of the control group, and the expression of TH-mRNA was not obvious in the control group but was strong in the induced group. There were significant differences between the two groups. Conclusion Human fetal striatal extracts can effectively induce human fetal NSCs to differentiate into dopaminergic neurons in vitro.

Key words: Neural stem cells, Human embryo, Dopaminergic neuron, Differentiation, Parkinson′s disease

CLC Number: 

  • Q813.1
[1] LI Jingyuan, SONG Ling, LIN Song, SONG Hui. Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 31-37.
[2] LI Shanshan, YANG Jing, ZHANG Jin. Post-transcriptional regulation mechanism of sclerostin in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 43-48.
[3] FAN Fengjing, ZHANG Guangying, CAO Shujuan, DING Hongyu, LIU Dequan. Diagnostic value of virtual touch tissue quantification in the differential diagnosis of breast tumors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 48-52.
[4] CAO Kai, WANG Chaoqun, ZHENG Maojin, WU Yongping. Correlation between TLR4, MyD88 and ERK1/2 expressions and EBV infected colorectal cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 75-79.
[5] YANG Bo, LI Ping, MENG Liping, ZHOU Changzuan, PAN Sunlei, CHI Jufang, GUO Hangyuan. Enalapril inhibits homocysteine-induced smooth muscle cell dedifferentiation and its signal pathway [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 21-26.
[6] LIU Lianke, SHAO Mingwen, MA Lan, SUN Jing, GUAN Dan, SHU Yongqian. Clinico-pathological characteristics and diagnostic biomarkers in patients with esophageal carcinoma accompanied by neuroendocrine cell differentiation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 87-91.
[7] LIANG Yu, XIN Tao, LIU Qian, ZHENG Xiangrong, YANG Yihang, PANG Qi. Effects of theanine on the proliferation and differentiation of neural stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 49-54.
[8] FU Haiyan, HU Zhansheng, DU Hongyang, LI Chao, BAO Cuifen. Effect of rehmannia glutinosa polysaccharide on the differentiation and proliferation of over-expressed Notch1(NICD) gene in rat bone marrow mesenchymal stem cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 34-40.
[9] LI Guanhong1, WANG Wei1, LI Jiye1, A Sileng2, PAN Runhua1, LIAO Caixian1. H3K27 dimethylation changes during BMDLSCs differentiation into hepatocytes from rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 28-32.
[10] ZHU Yuhua1, YUAN Honghua2, WU Lianlian2, HU Ankang1, CHEN Renjin1, ZHU Xiaorong1. Insulin-like growth factor-1 induces neural stem cells to differentiate into neurons via PI3K/Akt signaling pathway [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 11-15.
[11] MENG Xiangji, PANG Qi, DING Feng, XIN Tao, YANG Hongan. Establishment of PD rat model by injecting Taclo sterically and directionally into striatum [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 16-18.
[12] FANG Min1, BI Ke-hong2, JIANG Guo-sheng3, ZHU Chuan-sheng2. Changes of the LMP/TAP gene expressions and its significance in leukemia cells HL-60 after induced differentiation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(2): 1-5.
[13] JIANG Lili, MA Zhe, MA Chuyun, HE Yuanliu, TAO Guowei, LIU Cun, GENG Qun, TANG Tingting, WANG Yin. Value of acoustic radiation force impulse imaging technology for differentiating benign from malignant thyroid nodules [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 72-76.
[14] HA Lingxia, LI Xianghong. Expression and significance of GDF9 and BMP15 in follicular fluid of women with polycysticovary syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 77-80,95.
[15] BAO Dan-dan, LI Ping, CHU Hong-shang, LIU Hui-juan. Effects of bone morphogenetic protein-2 on the proliferation and differentiation of neural stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(1): 10-14.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd