Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (4): 44-50.doi: 10.6040/j.issn.1671-7554.0.2024.0686

• Nutrition, Gut Microbiota, and Associated Diseases • Previous Articles    

Two-sample Mendelian randomization of the relationship between gut microbiota and the risk of extrahepatic cholangiocarcinoma

WANG Xiaolei1, FANG Jun1, WANG An1, ZHU Wuhui1, SHI Guangjun2   

  1. 1. Department of Hepatobiliary Surgery, Qingdao Third Peoples Hospital, Qingdao 266041, Shandong, China;
    2. Department of Hepatobiliary Surgery, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China
  • Published:2025-04-08

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Abstract: Objective To evaluate the causal relationship between gut microbiota and extrahepatic cholangiocarcinoma(ECC)by using two-sample Mendelian randomization(MR)analysis. Methods Genome-wide association study(GWAS)data of human gut microbiota were obtained from the official website of MiBioGen Alliance as exposure factors, and GWAS data of ECC were obtained from the official website of GWAS Catalog as the outcome. Five methods, including random effect inverse variance weighting, weighted median, MR-Egger regression, simple model and weighted model, were used to conduct two-sample MR to explore the causal relationship between gut microbiota and the risk of ECC. Sensitivity analysis, heterogeneity test and pleiotropy test were also conducted to evaluate the reliability and accuracy of the results. Results Finally, 55 instrumental variables related to gut microbiota were selected. MR results showed that the Oscillospira(OR=6.705, 95%CI: 1.612-27.890, P=0.009), Prevotella 7(OR=2.565, 95%CI: 1.108-5.940, P=0.028) and Family_XIII_AD3011_group(OR=5.513, 95%CI: 1.186-25.620, P=0.029)were positively correlated with ECC. There was a negative causal relationship between Acidaminococcaceae(OR=0.113, 95%CI: 0.026-0.500, P=0.004), Lactobacillaceae(OR=0.349, 95%CI: 0.132-0.924, P=0.034), Butyrivibrio(OR=0.493, 95%CI: 0.252-0.963, P=0.039)and ECC. The direction of MR Egger, weighted median method, simple model and weighted model was consistent with inverse variance weighted method. Sensitivity analysis showed no horizontal pleiotropy and heterogeneity, and MR results were reliable. Conclusion There is a potential causal relationship between Oscillospira, Prevotella 7, Family_XIII_AD3011_group, Acidaminococcaceae, Lactobacillaceae, Butyrivibrio and ECC.

Key words: Gut microbiota, Extrahepatic cholangiocarcinoma, Mendelian randomization, Genome-wide association study, Causal relationship

CLC Number: 

  • R735.7
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