Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (11): 20-26.doi: 10.6040/j.issn.1671-7554.0.2023.0900

• Academic Frontiers • Previous Articles     Next Articles

Progress in the establishment and application of organoids of bladder cancer

Andong GUO1,Sentai DING1,2,*()   

  1. 1. Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250012, Shandong, China
    2. Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2023-10-13 Online:2023-11-10 Published:2023-12-12
  • Contact: Sentai DING E-mail:dingsentai@126.com

RichHTML

15

PDF (PC)

192

Abstract:

Bladder cancer is a common disease of the urinary system. Cisplatin-based chemotherapy is the first-line treatment for inoperable and metastatic myometrial invasive bladder cancer. However, due to resistance, a large number of patients fail in chemotherapy, which leads to tumor recurrence and progression. In recent years, organoid models have become a hot spot in the research of pathogenesis, metastasis and drug sensitivity. The successful establishment of bladder cancer organoids is a breakthrough in the clinical treatment of bladder cancer, because organoids and primary tissues have a high degree of genetic and phenotypic consistency, which can help us better understand the genomic changes of bladder cancer, and detect drug sensitivity and resistance. This article aims to review and analyze the construction process, characteristics, advantages and applications of bladder cancer organoids as preclinical models.

Key words: Organoids, Bladder cancer, Drug screening, Tumor pathogenesis, Precision medicine

CLC Number: 

  • R737.14

Fig.1

Culture and application of bladder cancer organoids (created with BioRender.com)"

1 王凯剑, 戴利和, 许传亮. 膀胱癌分子分型的研究进展[J]. 第二军医大学学报, 2018, 39 (1): 81- 85.
doi: 10.16781/j.0258-879x.2018.01.0081
WANG Kaijian , DAI Lihe , XU Chuanliang . Molecular typing of bladder cancer: an update[J]. Academic Journal of Second Military Medical University, 2018, 39 (1): 81- 85.
doi: 10.16781/j.0258-879x.2018.01.0081
2 Jubber I , Ong S , Bukavina L , et al. Epidemiology of bladder cancer in 2023: a systematic review of risk factors[J]. Eur Urol, 2023, 84 (2): 176- 190.
doi: 10.1016/j.eururo.2023.03.029
3 Witjes JA , Bruins HM , Cathomas R , et al. European association of urology guidelines on muscle-invasive and metastatic bladder cancer: summary of the 2020 guidelines[J]. Eur Urol, 2021, 79 (1): 82- 104.
doi: 10.1016/j.eururo.2020.03.055
4 Compérat E , Amin MB , Cathomas R , et al. Current best practice for bladder cancer: a narrative review of diagnostics and treatments[J]. Lancet, 2022, 400 (10364): 1712- 1721.
doi: 10.1016/S0140-6736(22)01188-6
5 刘润泽, 张勇. 患者源性膀胱癌类器官模型的应用及研究进展[J]. 中华肿瘤防治杂志, 2023, 30 (15): 948- 952.
doi: 10.16073/j.cnki.cjcpt.2023.15.09
LIU Runze , ZHANG Yong . Application and research progress of patient-derived organoid model in bladder cancer[J]. Chinese Journal of Cancer Prevention and Treatment, 2023, 30 (15): 948- 952.
doi: 10.16073/j.cnki.cjcpt.2023.15.09
6 Clevers H . Modeling development and disease with organoids[J]. Cell, 2016, 165 (7): 1586- 1597.
doi: 10.1016/j.cell.2016.05.082
7 Huang L , Holtzinger A , Jagan I , et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell and patient-derived tumor organoids[J]. Nat Med, 2015, 21 (11): 1364- 1371.
doi: 10.1038/nm.3973
8 Homan KA , Gupta N , Kroll KT , et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro[J]. Nat Methods, 2019, 16 (3): 255- 262.
doi: 10.1038/s41592-019-0325-y
9 Roelofs C , Hollande F , Redvers R , et al. Breast tumour organoids: promising models for the genomic and functional characterisation of breast cancer[J]. Biochem Soc Trans, 2019, 47 (1): 109- 117.
doi: 10.1042/BST20180375
10 Lhmussaar K , Kopper O , Korving J , et al. Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids[J]. Nat Commun, 2020, 11 (1): 2660.
doi: 10.1038/s41467-020-16432-0
11 Lancaster MA , Knoblich JA . Generation of cerebral organoids from human pluripotent stem cells[J]. Nat Protoc, 2014, 9 (10): 2329- 2340.
doi: 10.1038/nprot.2014.158
12 Barker N , Huch M , Kujala P , et al. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro[J]. Cell Stem Cell, 2010, 6 (1): 25- 36.
doi: 10.1016/j.stem.2009.11.013
13 孙晓宇, 张志宏, 张昌文. 膀胱癌类器官模型的研究进展[J]. 天津医科大学学报, 2023, 29 (5): 564- 567.
14 Zhao X , Jiang Y , Liu C , et al. Organoid technology and clinical applications in digestive system cancer[J]. Engineering, 2022, 9 (2): 123- 130.
15 Medle B , Sj dahl G , Eriksson P , et al. Patient-derived bladder cancer organoid models in tumor biology and drug testing: a systematic review[J]. Cancers (Basel), 2022, 14 (9): 2062.
doi: 10.3390/cancers14092062
16 Bentivegna A , Conconi D , Panzeri E , et al. Biological heterogeneity of putative bladder cancer stem-like cell populations from human bladder transitional cell carcinoma samples[J]. Cancer Sci, 2010, 101 (2): 416- 424.
doi: 10.1111/j.1349-7006.2009.01414.x
17 Kim E , Choi S , Kang B , et al. Creation of bladder assembloids mimicking tissue regeneration and cancer[J]. Nature, 2020, 588 (7839): 664- 669.
doi: 10.1038/s41586-020-3034-x
18 Yu L , Li Z , Mei H , et al. Patient-derived organoids of bladder cancer recapitulate antigen expression profiles and serve as a personal evaluation model for CAR-T cells in vitro[J]. Clin Transl Immunology, 2021, 10 (2): e1248.
doi: 10.1002/cti2.1248
19 Walz S , Pollehne P , Geng R , et al. A protocol for organoids from the urine of bladder cancer patients[J]. Cells, 2023, 12 (17): 2188.
doi: 10.3390/cells12172188
20 Hofner T , Macher-Goeppinger S , Klein C , et al. Development and characteristics of preclinical experimental models for the research of rare neuroendocrine bladder cancer[J]. J Urol, 2013, 190 (6): 2263- 2270.
doi: 10.1016/j.juro.2013.06.053
21 Lee SH , Hu W , Matulay JT , et al. Tumor evolution and drug response in patient-derived organoid models of bladder cancer[J]. Cell, 2018, 173 (2): 515- 528.
doi: 10.1016/j.cell.2018.03.017
22 Shen L , Zhang J , Zheng Z , et al. PHGDH inhibits ferroptosis and promotes malignant progression by upregulating SLC7A11 in bladder cancer[J]. Int J Biol Sci, 2022, 18 (14): 5459- 5474.
doi: 10.7150/ijbs.74546
23 Wang M , Chen X , Tan P , et al. Acquired semi-squamatization during chemotherapy suggests differentiation as a therapeutic strategy for bladder cancer[J]. Cancer Cell, 2022, 40 (9): 1044- 1059.
doi: 10.1016/j.ccell.2022.08.010
24 Mullenders J , de Jongh E , Brousali A , et al. Mouse and human urothelial cancer organ-oids: a tool for bladder cancer research[J]. Proc Natl Acad Sci U S A, 2019, 116 (10): 4567- 4574.
doi: 10.1073/pnas.1803595116
25 Wang N , Chen RX , Deng MH , et al. M5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization[J]. Cell Death Dis, 2023, 14 (2): 139.
doi: 10.1038/s41419-023-05661-y
26 Geng R , Harland N , Montes-Mojarro IA , et al. CD24: a marker for an extended expansion potential of urothelial cancer cell organoids in vitro[J]. Int J Mol Sci, 2022, 23 (10): 5453.
doi: 10.3390/ijms23105453
27 Xiao K , Peng S , Lu J , et al. UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer[J]. Cell Death Dis, 2023, 14 (7): 408.
doi: 10.1038/s41419-023-05938-2
28 Vlaar JM , Borgman A , Kalkhoven E , et al. Recurrent exon-deleting activating mutations in AHR act as drivers of urinary tract cancer[J]. Sci Rep, 2022, 12 (1): 10081.
doi: 10.1038/s41598-022-14256-0
29 Kong J , Lee H , Kim D , et al. Network-based machine learning in colorectal and bladder organoid models predicts anti-cancer drug efficacy in patients[J]. Nat Commun, 2020, 11 (1): 5485.
doi: 10.1038/s41467-020-19313-8
30 Burgués JP , Gómez L , Pontones JL , et al. A chemosensitivity test for superficial bladder cancer based on three-dimensional culture of tumour spheroids[J]. Eur Urol, 2007, 51 (4): 962- 970.
doi: 10.1016/j.eururo.2006.10.034
31 Neal JT , Li X , Zhu J , et al. Organoid modeling of the tumor immune microenvironment[J]. Cell, 2018, 175 (7): 1972- 1988.
doi: 10.1016/j.cell.2018.11.021
32 Elbadawy M , Sato Y , Mori T , et al. Anti-tumor effect of trametinib in bladder cancer organoid and the underlying mechanism[J]. Cancer Biol Ther, 2021, 22 (5-6): 357- 371.
doi: 10.1080/15384047.2021.1919004
33 Abugomaa A , Elbadawy M , Ishihara Y , et al. Anti-cancer activity of Chaga mushroom (Inonotus obliquus) against dog bladder cancer organoids[J]. Front Pharmacol, 2023, 14, 1159516.
doi: 10.3389/fphar.2023.1159516
34 Gelbrich N , Miebach L , Berner J , et al. Medical gas plasma augments bladder cancer cell toxicity in preclinical models and patient-derived tumor tissues[J]. J Adv Res, 2023, 47, 209- 223.
doi: 10.1016/j.jare.2022.07.012
35 Seiler R , Egger M , De Menna M , et al. Guidance of adjuvant instillation in intermediate-risk non-muscle invasive bladder cancer by drug screens in patient derived organoids: a single center, open-label, phase Ⅱ trial[J]. BMC Urol, 2023, 23 (1): 89.
doi: 10.1186/s12894-023-01262-1
36 Becker L , Fischer F , Fleck JL , et al. Data-driven identification of biomarkers for in situ monitoring of drug treatment in bladder cancer organoids[J]. Int J Mol Sci, 2022, 23 (13): 6956.
doi: 10.3390/ijms23136956
37 Gong Z , Huang L , Tang X , et al. Acoustic droplet printing tumor organoids for modeling bladder tumor immune microenvironment within a week[J]. Adv Healthc Mater, 2021, 10 (22): e2101312.
doi: 10.1002/adhm.202101312
38 Yoon WH , Lee HR , Kim S , et al. Use of inkjet-printed single cells to quantify intratumoral heterogeneity[J]. Biofabrication, 2020, 12 (3): 035030.
doi: 10.1088/1758-5090/ab9491
39 Gheibi P , Zeng S , Son KJ , et al. Microchamber cultures of bladder cancer: a platform for characterizing drug responsiveness and resistance in PDX and primary cancer cells[J]. Sci Rep, 2017, 7 (1): 12277.
doi: 10.1038/s41598-017-12543-9
40 Lee LM , Seftor EA , Bonde G , et al. The fate of human malignant melanoma cells transplanted into zebrafish embryos: assessment of migration and cell division in the absence of tumor formation[J]. Dev Dyn, 2005, 233 (4): 1560- 1570.
doi: 10.1002/dvdy.20471
41 Marques IJ , Weiss FU , Vlecken DH , et al. Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model[J]. BMC Cancer, 2009, 9, 128.
doi: 10.1186/1471-2407-9-128
42 Ali Z , Vildevall M , Rodriguez GV , et al. Zebrafish patient-derived xenograft models predict lymph node involvement and treatment outcome in non-small cell lung cancer[J]. J Exp Clin Cancer Res, 2022, 41 (1): 58.
doi: 10.1186/s13046-022-02280-x
43 Wu ZS , Wu S . The era of personalized treatments: updates on immunotherapy within urothelial of bladder cancer[J]. Curr Urol, 2022, 16 (3): 117- 120.
doi: 10.1097/CU9.0000000000000133
[1] WANG Linlin, SUN Yuping. From the perspective of clinicians: the application and reflection of artificial intelligence in cancer precision diagnosis and treatment [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 89-96.
[2] YANG Jun. Medico-engineering cooperation: a strategic plateau of neurosurgery innovation and development in the era of precision medicine [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 83-88.
[3] WANG Chuanxin. Liquid biopsy of tumor [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 64-71.
[4] MI Qi, SHI Shuang, LI Juan, LI Peilong, DU Lutao, WANG Chuanxin. Construction of circRNA-mediated ceRNA network and prognostic assessment model for bladder cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 94-102.
[5] GAO Jinmei, ZHANG Xianglian, LIU Tieju. Correlation between plasma D-dimer and metastasis in 109 cases of bladder cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 98-102.
[6] LIANG Li, SUN Bin, ZHANG Hongzhan, YE Fangzhi, FENG Xianguang, ZHANG Kai. Analysis of clinical characteristics of 48 cases of gallbladder adenoma based on different pathological grades [J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 59-63.
[7] ZHANG Zhaopeng, XING Naidong, ZHANG Xiang, YAN Lei, XU Zhonghua. Influencing factors of lymphatic leakage after laparoscopic radical cystectomy: an analysis of 160 cases [J]. Journal of Shandong University (Health Sciences), 2020, 58(1): 67-72.
[8] WANG Jian, LI Jian, WANG Yong, ZHU Yaofeng. Baicalein induces apoptosis of bladder cancer cells via inhibition of the PI3K/AKT/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2019, 57(9): 74-82.
[9] WANG Haitao. Development of precision medicine in the treatment of castration-resistant prostate cancer [J]. Journal of Shandong University (Health Sciences), 2019, 57(1): 30-35.
[10] LIU Jie, CUI Wei, CHE Zi, CUI Zhiqiang, WANG Cezheng, WANG Tong, LI Ming, LIU Ling, YANG Quancheng, SUN Bin, GAO Dianjun, Nie Qingsheng. Efficacy of transurethral resection of bladder tumor in the treatment of muscle-invasive bladder cancer [J]. Journal of Shandong University (Health Sciences), 2018, 56(7): 81-85.
[11] LIU Yaxiao, SHI Benkang. Effects of capsaicin on the invasion and metastasis of bladder cancer [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 34-40.
[12] HAO Kuiyuan, ZHAO Sheng, ZHANG Yu, CUI Di, JING Yifeng, XIA Shujie, HAN Bangmin. Effect of androgen blockade on autophagy and apoptosis in bladder cancer UM-UC-3 cells [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 41-47.
[13] WANG Hongyang. Development of tumor biomarkers in the age of precision medicine [J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 1-2.
[14] YIN Lei, YIN Rui, LI Wenjia, LIU Shuai, L(¨overU)Jiaju. CYLD improves bladder cancer gemcitabine chemosensitivity via suppressing autophagy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 1-6.
[15] LIU Yimin, DU Lutao, WANG Lili, JIANG Xiumei, LI Juan, QU Ailin, WANG Haiyan, ZHENG Guixi, ZHANG Xin,YANG Yongmei, WANG Chuanxin. Identification and validation of suitable reference genes for the investigation of serum microRNA in bladder cancer patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 86-91.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15 -23 .
[2] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38 -46 .
[3] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7 -14 .
[4] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24 -31 .
[5] SHI Shuang, LI Juan, MI Qi, WANG Yunshan, DU Lutao, WANG Chuanxin. Construction and application of a miRNAs prognostic risk assessment model of gastric cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 47 -52 .
[6] XIAO Juan, XIAO Qiang, CONG Wei, LI Ting, DING Shouluan, ZHANG Yuan, SHAO Chunchun, WU Mei, LIU Jianing, JIA Hongying. Comparison of diagnostic efficacy of two kinds of thyroid imagine reporting and data systems[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 53 -59 .
[7] LI Songlin, LIU Peilai, LU Qunshan, MA Heran. Application of high tibial osteotomy combined with autologous adipose-derived mesenchymal stem cells injection in the repair of knee cartilage[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 82 -88 .
[8] XU Jixi, CHEN Weijian. Diffuse midline glioma with H3 K27M mutation in the spinal cord: a case report[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 96 -101 .
[9] Tao JIANG. The application of brain-like intelligence in the frontiers of brain science[J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 10 -13 .
[10] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1 -6 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd