Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (9): 47-55.doi: 10.6040/j.issn.1671-7554.0.2023.0524

• Preclinical Medicine • Previous Articles     Next Articles

PM2.5 induces inflammatory response in nasal epithelial cells through TLR4/NF-κB pathway

YANG Xiaozhe1,2, ZHAO Yan1,2, QING Hui3, WANG Xiangdong1,2,3, ZHANG Luo1,2,3   

  1. 1. Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University), Ministry of Education, Beijing 100730, China;
    2. Beijing Institute of Otolaryngology, Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission, Beijing Key Laboratory of Nasal Diseases, Beijing 100005, China;
    3. Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
  • Received:2023-06-17 Published:2023-10-10

PDF (PC)

35

Abstract: Objective To investigate the mechanism of TLR4/NF-κB signaling pathway in the inflammatory response of nasal mucosal epithelial cells induced by fine particulate matter(PM2.5). Methods After healthy human nasal epithelial cells(HNEpCs)were exposed to PM2.5, the RNA was extracted for transcriptome analysis by Illumina sequencer. The expressions of TLR4, NFKB1 and NFKB1A were detected with quantitative real-time PCR(qRT-PCR). Results PM2.5 caused cell toxicity and abnormal regulation of transcriptome of HNEpCs, and abnormal expressions of TLR4, NFKBIA and IL1B. KEGG pathway analysis indicated that TLR4/NF-κB pathway was significantly enriched. The qRT-PCR validation experiment revealed that after PM2.5 exposure, TLR4 was up-regulated while NFKB1 was down-regulated, and NFKBIA,which inhibited NF-κB, was significantly down-regulated, thus leading to abnormal regulation of the TLR4/NF-κB pathway. Conclusion The abnormal regulation of TLR4/NF-κB in HNEpCs may play a crucial role in the nasal mucosa inflammatory response induced by PM2.5 exposure.

Key words: Nasal epithelial cell line, Fine particulate matter, Exposure model, Abnormal regulation of gene, Quantitative red-time, Transcriptome analysis, Nasal mucosa inflammation

CLC Number: 

  • R765.25
[1] Zhou MG, Wang HD, Zeng XY, et al. Mortality, morbidity, and risk factors in China and its provinces, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017 [J]. Lancet, 2019, 394(10204): 1145-1158. doi: 10.1016/S0140-6736(19)30427-1.
[2] Liang FC, Xiao QY, Huang KY, et al. The 17-y spatiotemporal trend of PM2.5 and its mortality burden in China [J]. Proc Natl Acad Sci USA, 2020, 117(41): 25601-25608. doi: 10.1073/pnas.1919641117.
[3] 王得翔, 季秀丽, 马德东, 等. 济南地区支气管哮喘患者经济负担及其影响因素分析[J]. 山东大学学报(医学版), 2012, 50(5): 124-128. WANG Dexiang, JI Xiuli, MA Dedong, et al. The economic burden of bronchial asthma and its related factors in Jinan [J]. Journal of Shandong University(Health Sciences), 2012, 50(5): 124-128.
[4] Mady LJ, Schwarzbach HL, Moore JA, et al. Air pollutants may be environmental risk factors in chronic rhinosinusitis disease progression [J]. Int Forum Allergy Rhinol, 2018, 8(3): 377-384.
[5] Park EJ, Roh J, Kang MS, et al. Biological responses to diesel exhaust particles(DEPs)depend on the physicochemical properties of the DEPs [J]. PLoS One, 2011, 6(10): e26749. doi: 10.1371/journal.pone.0026749.
[6] 杨似玉, 闫晓娜, 彭靖, 等. 郑州市两城区大气PM2.5中金属、类金属污染特征及健康风险评估[J]. 山东大学学报(医学版), 2021, 59(12): 70-77. doi: 10.6040/j.issn.1671-7554.0.2021.1079. YANG Siyu, YAN Xiaona, PENG Jing, et al. Pollution characteristics and health risk assessment of metals and metalloids in PM2.5 in two districts of Zhengzhou [J]. Journal of Shandong University(Health Science), 2021, 59(12): 70-77.
[7] Guo ZQ, Dong WY, Xu J, et al. T-helper type 1-T-helper type 2 shift and nasal remodeling after fine particulate matter exposure in a rat model of allergic rhinitis [J]. Am J Rhinol Allergy, 2017, 31(3): 148-155.
[8] Ogata H, Goto S, Sato K, et al. KEGG: Kyoto encyclopedia of genes and genomes [J]. Nucleic Acids Res, 1999, 27(1): 29-34.
[9] Gu WJ, Hou TH, Zhou HW, et al. Ferroptosis is involved in PM2.5-induced acute nasal epithelial injury via AMPK-mediated autophagy [J]. Int Immunopharmacol, 2023, 115: 109658. doi: 10.1016/j.intimp.2022.109658.
[10] Hong ZC, Guo ZQ, Zhang RX, et al. Airborne fine particulate matter induces oxidative stress and inflammation in human nasal epithelial cells [J]. Tohoku J Exp Med, 2016, 239(2): 117-125.
[11] Xian M, Ma SY, Wang KJ, et al. Particulate matter 2.5 causes deficiency in barrier integrity in human nasal epithelial cells [J]. Allergy Asthma Immunol Res, 2020, 12(1): 56-71.
[12] Xian M, Wang KJ, Lou HF, et al. Short-term haze exposure predisposes healthy volunteers to nasal inflammation [J]. Allergy Asthma Immunol Res, 2019, 11(5): 632-643.
[13] Chu H, Mazmanian SK. Innate immune recognition of the microbiota promotes host-microbial symbiosis [J]. Nat Immunol, 2013, 14(7): 668-675.
[14] Osterlund C, Grönlund H, Polovic N, et al. The non-proteolytic house dust mite allergen Der p 2 induce NF-kappaB and MAPK dependent activation of bronchial epithelial cells [J]. Clin Exp Allergy, 2009, 39(8): 1199-1208.
[15] Choi HJ, Park SY, Cho JH, et al. The TLR4-associated phospholipase D1 activation is crucial for Der f 2-induced IL-13 production [J]. Allergy, 2015, 70(12): 1569-1579.
[16] Sun Y, Zhou B, Wang CS, et al. Biofilm formation and Toll-like receptor 2, Toll-like receptor 4, and NF-kappaB expression in sinus tissues of patients with chronic rhinosinusitis [J]. Am J Rhinol Allergy, 2012, 26(2): 104-109.
[17] Zhang Q, Wang CS, Han DM, et al. Differential expression of Toll-like receptor pathway genes in chronic rhinosinusitis with or without nasal polyps [J]. Acta Otolaryngol, 2013, 133(2): 165-173.
[18] Shimizu S, Kouzaki H, Kato T, et al. HMGB1-TLR4 signaling contributes to the secretion of interleukin 6 and interleukin 8 by nasal epithelial cells [J]. Am J Rhinol Allergy, 2016, 30(3): 167-172.
[19] Dai PY, Shen D, Shen JK, et al. The roles of Nrf2 and autophagy in modulating inflammation mediated by TLR4 - NFκB in A549 cell exposed to layer house particulate matter 2.5(PM2.5)[J]. Chemosphere, 2019, 235: 1134-1145. doi: 10.1016/j.chemosphere.2019.07.002.
[20] Becker S, Dailey L, Soukup JM, et al. TLR-2 is involved in airway epithelial cell response to air pollution particles [J]. Toxicol Appl Pharmacol, 2005, 203(1): 45-52.
[21] Wang HT, Song LY, Ju WH, et al. The acute airway inflammation induced by PM2.5 exposure and the treatment of essential oils in Balb/c mice [J]. Sci Rep, 2017, 7: 44256. doi:10.1038/srep44256.
[22] Wang X, Zhao CY, Ji WJ, et al. Relationship of TLR2, TLR4 and tissue remodeling in chronic rhinosinusitis [J]. Int J Clin Exp Pathol, 2015, 8(2): 1199-1212.
[23] Taziki MH, Azarhoush R, Taziki MM, et al. Correlation between HMGB1 and TLR4 expression in sinonasal mucosa in patients with chronic rhinosinusitis [J]. Ear Nose Throat J, 2019, 98(10): 599-605.
[24] Qing H, Wang XD, Zhang N, et al. The effect of fine particulate matter on the inflammatory responses in human upper airway mucosa [J]. Am J Respir Crit Care Med, 2019, 200(10): 1315-1318.
[25] Qiu FF, Liang CL, Liu HZ, et al. Impacts of cigarette smoking on immune responsiveness: up and down or upside down? [J]. Oncotarget, 2017, 8(1): 268-284.
[26] Zhao C, Liao JP, Chu WL, et al. Involvement of TLR2 and TLR4 and Th1/Th2 shift in inflammatory responses induced by fine ambient particulate matter in mice [J]. Inhal Toxicol, 2012, 24(13): 918-927.
[27] Shoenfelt J, Mitkus RJ, Zeisler R, et al. Involvement of TLR2 and TLR4 in inflammatory immune responses induced by fine and coarse ambient air particulate matter [J]. J Leukoc Biol, 2009, 86(2): 303-312.
[1] ZHAI Yifan, WANG Zhaojun, BAI Shuoxin, LIN Shaoqian, WANG Fangyi, DU Shuang, WANG Zhiping. Effect of maternal PM10 and PM2.5 exposure during pregnancy on neonatal birth weight [J]. Journal of Shandong University (Health Sciences), 2021, 59(8): 99-106.
[2] XIAO Yang, TAO Yu, WANG Fangyi, LIANG Yuxiu, ZHANG Jin, JI Xiaokang, WANG Zhiping. Association between PM2.5 and PM10 exposure with gestational diabetes mellitus in certain areas of Shandong Province [J]. Journal of Shandong University (Health Sciences), 2021, 59(12): 101-109.
[3] WANG Liheng, PENG Xiumiao, ZHANG Yingjian, SHAN Bing, CAO Meng, CUI Liangliang. Concentration characteristics and chronic health risk assessment of metal elements in atmospheric PM2.5 in two districts of Jinan from 2016 to 2020 [J]. Journal of Shandong University (Health Sciences), 2021, 59(12): 63-69.
[4] SUN Chengyao, TANG Dajing, CHEN Fengge, ZHAO Chuan, GUAN Mingyang. Trend and health risk assessment of chemical components in atmospheric PM2.5 in Shijiazhuang City from 2016 to 2020 [J]. Journal of Shandong University (Health Sciences), 2021, 59(12): 78-86.
[5] CAO Meng, WANG Liheng, PENG Xiumiao, CUI Liangliang. Concentration characteristics and chronic health risk assessment of polycyclic aromatic hydrocarbons in atmospheric PM2.5 in two districts of Jinan from 2016 to 2020 [J]. Journal of Shandong University (Health Sciences), 2021, 59(12): 87-95.
[6] WU Weidong, AN Zhen, GUI Shuangjun, XU Jie, FAN Wei, SONG Jie. PM2.5 exposure promotes the genesis and development of atherosclerosis [J]. Journal of Shandong University (Health Sciences), 2018, 56(11): 18-26.
[7] LIU Xiaoli, LIU Fangying, MENG Chao, LI Ping, ZHANG Dianping, YIN Maorong, ZHAI Shenyong. Time series analysis on the relationship between air pollution and daily emergency room visits in Zibo City [J]. Journal of Shandong University (Health Sciences), 2018, 56(11): 42-47.
[8] KONG Fanling, ZHAO Lin, CUI Liangliang. PM2.5 and cardiovascular disease [J]. Journal of Shandong University (Health Sciences), 2018, 56(11): 12-17.
[9] SHI Xiaoming. Research advances on the epidemiological study on acute health effects of ambient fine particulate matter and related components [J]. Journal of Shandong University (Health Sciences), 2018, 56(11): 1-11.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1 -6 .
[2] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7 -14 .
[3] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24 -31 .
[4] XU Yuxiang, LIU Yudong, ZHANG Peng, DUAN Ruisheng. A retrospective analysis of risk factors of cerebral microbleeds in 101 patients with cerebral small vessel disease[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 67 -71 .
[5] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15 -23 .
[6] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38 -46 .
[7] XIAO Juan, XIAO Qiang, CONG Wei, LI Ting, DING Shouluan, ZHANG Yuan, SHAO Chunchun, WU Mei, LIU Jianing, JIA Hongying. Comparison of diagnostic efficacy of two kinds of thyroid imagine reporting and data systems[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 53 -59 .
[8] LYU Longfei, LI Lin, LI Shuhai, QI Lei, LU Ming, CHENG Chuanle, TIAN Hui. Application of laparoscopic fine needle catheter jejunostomy in minimally invasive McKeown resection of esophageal cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 77 -81 .
[9] ZHANG Juan, ZHANG Lujia, XIAO Wei, LI Shunping. Influencing factors of perceived stress and job retention in national standardized training for resident doctors[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 108 -114 .
[10] SHI Shuang, LI Juan, MI Qi, WANG Yunshan, DU Lutao, WANG Chuanxin. Construction and application of a miRNAs prognostic risk assessment model of gastric cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 47 -52 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd