Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (7): 34-39.doi: 10.6040/j.issn.1671-7554.0.2023.0272

• 基础医学 • Previous Articles    

Influencing factors of PC12 cell injury induced by high glucose and the protective effects of catalpol

LIU Fengqi*, GAO Feng*, XUE Caicai, QIAO Xiumei, WANG Jinhong   

  1. School of Pharmacy, Weifang Medical University, Weifang 261053, Shandong, China
  • Published:2023-07-04

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Abstract: Objective To investigate the damaging effects of high glucose(HG)on rat adrenal pheochromocytoma PC12 cells under different conditions, to analyze the influencing factors of establishing a model of cell injury induced by HG, and to explore the protective effects of catalpol. Methods PC12 cells were seeded with a density of 1×103, 1×104, 1×105 cells/mL. HG was added into the medium at 24 and 48 hours after seeding. The final concentrations of glucose were 30, 40, 50 and 75 mmol/L. PC12 cells damaged by HG were divided into HG30, HG40, HG50 and HG75 groups accordingly. Control cells were treated with equal volume of physiological saline. After HG acted for 24, 48, and 72 hours, respectively, the cell survival rate was measured with MTT. After the cell injury model was established, cells were divided into control group, model group, low catalpol dose group(1×10-5mol/L)and high catalpol dose group(1×10-4mol/L). The reactive oxygen species(ROS)content was detected with ELISA and fluorescent probe. The lactate dehydrogenase(LDH)content was measured with ELISA. Results Under different glucose conditions, compared with the control group, the cells in HG30 group had unchanged(P>0.05)or decreased survival rate(P<0.05), while the cells in HG40, HG50, and HG75 groups had decreased survival rate(P<0.05 or P<0.01). Compared with the control group, the model group had increased ROS and LDH contents(both P<0.01). Compared with model group, the low and high catalpol dose groups had increased survival rate(P<0.01), and decreased ROS and LDH contents(P<0.01). Conclusion Injury in PC12 cells induced by HG is influenced by factors such as cell planting density, glucose concentration and action duration. The HG induced PC12 cell injury model can be established stably by a final concentration of 50 mmol/L glucose acting on cells seeded with a density of 1×104 cells/mL for 24 hours. Catalpol plays a protective role on the model.

Key words: High glucose, Cell injury model, Influencing factors, PC12 cells, Reactive oxygen species

CLC Number: 

  • R574
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