Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (9): 19-28.doi: 10.6040/j.issn.1671-7554.0.2023.0243

• Preclinical Medicine • Previous Articles     Next Articles

Clinical value of serum exosomal LINC02163 as a marker for distant metastasis of colorectal cancer

DONG Yaqi1,2, WANG Xinhui1,2, ZHAO Yinghui1,2,3, WANG Chuanxin1,2   

  1. 1. Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, China;
    2. Shandong Provincial Key Laboratory of Innovation Technology in Laboratory Medicine, Jinan 250033, Shandong, China;
    3. Suzhou Research Institute of Shandong University, Suzhou 215123, Jiangsu, China
  • Received:2023-03-21 Published:2023-10-10

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Abstract: Objective To investigate the correlation between long non-coding RNA(lncRNA)LINC02163 and distant metastasis of colorectal cancer and its biological function, and to evaluate the potential of serum exosomal LINC02163 as a marker for distant metastasis. Methods The differential lncRNAs was screened by analyzing the transcriptome data of colorectal cancer tissues. The clinicopathological parameters and demographic parameters of patients were combined to analyze the expression characteristics of LINC02163. The correlation between LINC02163 and prognosis was investigated by in situ hybridization(ISH)in colorectal cancer microarray. The effects of LINC02163 on the distant metastasis of SW620 cells in vivo was investigated by establishing a tumor metastasis model in nude mice. Serum exosomes were identified with transmission electron microscopy, nanoparticle tracking analysis and Western blotting. The stability of serum exosomal LINC02163 was analyzed with RNase A incubation assay. The receiver operating characteristic(ROC)curve was drawn to evaluate the efficacy of serum exosomal LINC02163 in the identification of distant metastasis of colorectal cancer. Results The high expression of LINC02163 in colorectal cancer was screened by five colorectal cancer tissue transcriptome data. TCGA data analysis demonstrated that the expression of LINC02163 was associated with distant metastasis of colorectal cancer(P<0.05). ISH assay showed that high expression of LINC02163 was associated with poor prognosis(P<0.05). Tumor metastasis model in nude mice revealed that LINC02163 knockdown inhibited the distant metastasis of SW620 cells in vivo(P=0.05). Experiments involving the characterization of exosomes showed that serum exosomes were effectively extracted. RNase A incubation demonstrated the high stability of serum exosomal LINC02163. ROC analysis indicated that the area under the curve of serum exosomal LINC02163 was 0.864 for distant metastatic colorectal cancer. Conclusion LINC02163 is highly expressed in colorectal cancer and is associated with distant metastasis. Serum exosomal LINC02163 may become a marker of distant metastasis.

Key words: Colorectal cancer, Distant metastasis, Exosome, Long non-coding RNA, Marker

CLC Number: 

  • R735.3
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