Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (10): 27-32.doi: 10.6040/j.issn.1671-7554.0.2022.0152

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Long non-coding RNA NONHSAT247814.1 expression in 18 childhood with myocarditis and in vitro cellular experimental observation

FENG Xinxin1, HAN Bo1,2, ZHANG Li1, MA Mengjie1, CHEN Siyu2   

  1. 1. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong, China
  • Published:2022-09-30

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Abstract: Objective To explore the potential diagnostic marker of child myocarditis and to clarify the biological functions in human cardiomyocyte inflammation. Methods Long non-coding RNAs(LncRNAs)differentially expressed in the results of pediatric myocarditis gene chips were screened, the sample size was expanded for validation, and the target molecule was identified. The basic data and clinical information of 18 children with myocarditis(myocarditis group)and 18 healthy children(normal control group)were collected, real-time quantitative polymerase chain reaction(qRT-PCR)was used to test the expression of the target molecule, and then the receiver operating characteristic(ROC)curve was plotted to evaluate the value of the target molecule in the diagnosis of pediatric myocarditis. Lipopolysaccharide was utilized to stimulate AC16 cells and then extoblish the model of inflammatory injury, and the small interfering RNA was transfected into AC16 to inhibit the expression of the target molecule. Enzyme-linked immunosorbent assay(ELIAS)was used to test the protein concentration of inflammatory factors and myocardial injury markers in the cell supernatants, and CCK8 was used to detect cellular proliferation. Results NONHSAT247814.1 was identified as the target molecule. The relative expression of NONHSAT247814.1 was higher in the myocarditis group than in normal control group(P<0.001). The relative expression was higher in acute phase than in recovery phase, and positively correlated with NT-pro brain natriuretic peptide in the peripheral circulation(R2=0.40, P=0.005), but negatively correlated with left ventricular ejection fraction(R2=-0.53, P=0.0006). ROC for NONHSAT247814.1 in diagnosing myocarditis was(AUC=0.89, P<0.001, sensitivity=83.33% and specificity=88.89%). At the cellular level, reducing the expression of NONHSAT247814.1 attenuated the release of interleukin-1β, interleukin-6, troponin T and brain natriuretic peptide, while increased the cellular proliferation. Conclusion NONHSAT247814.1 has potential as a diognostic marker for myocarditis in children, inhibiting of NONHSAT247814.1 expression attenuates LPS-induced inflommation iujury in cardiomyocytes effects at the cellular level.

Key words: Long non-coding RNA, NONHSAT247814.1, Myocarditis, Diagnostic marker, Children

CLC Number: 

  • R725.4
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