Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (2): 25-30.doi: 10.6040/j.issn.1671-7554.0.2022.1324

• 基础医学 • Previous Articles     Next Articles

Effects of KLHL15 gene on the testis and liver of male mice

LIU Xiaohan, SHI Hui, ZHAO Zhenjun   

  1. Department of Biology, College of Life Science, Yantai University, Yantai 264005, Shandong, China
  • Published:2023-02-17

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Abstract: Objective To explore the effects of KLHL15 gene deletion on the structure and function of testis and liver in male mice. Methods The structure and function of testis and liver tissues of wild type(WT)and KLHL15 knockout type(KLHL15-/-)male mice were compared. The measurement indexes included: (1) Fertility: number of offspring, sperm vitality and sperm quality; (2) Anatomy: the mass and organ index of testis and liver were compared between WT and KLHL15-/- mice in similar growth conditions; (3) Histopathology: the effects of KLHL15 gene deletion on the structure of liver and testis were observed with HE staining; (4) Liver function: apoptosis of liver tissue was detected with Tunel; liver differential proteins were enriched and analyzed with TMT labeled quantitative proteomics. Results (1) Fertility: compared with WT mice, KLHL15-/- mice had decreased total sperm count, increased number of immobile sperm(IM), decreased average curve velocity(VCL)and average path velocity(VAP)(P=0.010, P=0.020). (2) Anatomy: compared with WT mice, KLHL15-/- mice had decreased mass of liver and testis(P<0.001, P=0.026), and decreased organ index(P<0.001); (3) Histopathology: the lumen of the convoluted seminiferous tubules of KLHL15-/- mice testis became larger, many spermatogenic cells were lost, the epithelial structure became thinner, and the number of sperm decreased; the liver cells were disorderly and the hepatic sinusoids were dilated; (4) Liver function: Tunel results showed more apoptotic cells in the liver of KLHL15-/- mice; enrichment analysis showed that deletion of KLHL15 gene affected the composition of mitochondria, various organelle membranes in the liver, reactions involved by peroxisomes and the metabolism of various amino acids. Conclusion Deletion of KLHL15 gene can destroy the structure of testis and reduce the sperm vitality and sperm quality of male mice, leading to reduced fertility. It can also cause structural damage to the liver of mice and affect the metabolic function of the liver.

Key words: KLHL15 gene, Knock out mice, Sperm quality, Liver, Testis

CLC Number: 

  • R588.1
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