Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (4): 43-50.doi: 10.6040/j.issn.1671-7554.0.2017.1010

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Total saponins of Panax ginseng promoting the anticancer activity of 5-Fluorouracil by mediating NK cell activity

MA Ranran1, HAN Chen2, WANG Zhaoxia2, WANG Zhaopeng2, GAO Yuxin2, ZHOU Shuping2, ZHANG Yueying2, LI Xiaobing2, LIU Hengyao1, WANG Hengxiao2   

  1. 1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China;
    2. Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the inhibitory effects of total saponins of Panax ginseng(TSPG)combined with 5-fluorouracil(5-Fu)on orthotopic transplantation tumors and the mechanism. Methods The orthotopic transplantation tumor models were established and randomly divided into 4 groups: model group, TSPG group, 5-Fu group and TSPG+5-Fu group. After 14 days, the livers were harvested and tumors were separated. The tumor weight, volume and tumor inhibition rate were determined. The pathological changes were observed with HE staining. The infiltration of NK cells in tumor tissues was observed with immunohistochemical method. The proportions of NK cells in the liver and spleen were assessed with flow cytometry. The mRNA expressions of perforin and granzyme B in tumor tissues were detected with real-time PCR. Results Compared with the model group, the other groups had significantly lower/smaller tumor volume and weight(P<0.05)and more obvious necrosis. The infiltration of NK cells increased in the TSPG group(P<0.05). The proportions of NK cells in the liver and spleen, and the mRNA expressions of perforin and Granzyme B increased in the TSPG group and TSPG+5-Fu group(P<0.05). Conclusion TSPG promotes the antitumor effects of 5-Fu, which may be explained by the increased number of NK cells and cytotoxity.

Key words: Total saponins of Panax ginseng, 5-fluorouracil, Orthotopic liver transplantation tumor, NK cell

CLC Number: 

  • R574
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